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Lilium oriental hybrid tissue culture seedling strengthening method

A technology of lily group and culture medium, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of easy re-infection of viruses, weak growth potential of tissue culture seedlings, slender and soft roots and leaves, etc., and achieves improved emergence. Quality, shortening of production cycle, effect of promoting bulking and weight gain

Active Publication Date: 2014-04-02
CHENGBANG ECO ENVIRONMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently in the factory production of lily bulbs, we often encounter prominent problems such as weak growth of lily tissue culture seedlings, slender and soft roots and leaves, slow bulb development, low survival rate during transplanting, and easy re-infection of viruses.

Method used

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  • Lilium oriental hybrid tissue culture seedling strengthening method
  • Lilium oriental hybrid tissue culture seedling strengthening method
  • Lilium oriental hybrid tissue culture seedling strengthening method

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Embodiment Construction

[0034] The present invention will be further explained below in combination with specific embodiments.

[0035] Brassinolide in the present invention was purchased from Shanghai Biochemical Reagent Factory, with a specification of 100 mg.

[0036] (1) Select the test-tube seedlings of the robust Oriental lily 'Sorbonne'.

[0037] (2) Some of the test-tube seedlings were inoculated in the basic propagation medium to propagate the initial seedlings. The basic propagation medium was: MS powder 4.43g / L+sucrose 60g / L+agar 6g / L, pH 5.6-5.7. The temperature of the culture room is 25±2°C, the light intensity is 1500 lux, and the light time is 12 hours / day.

[0038] (3) Select 0.5-0.8cm diameter sub-balls for the remaining part of the test-tube seedlings, gently cut off the roots, stems, and leaves with a scalpel, and then use the sub-balls as cue balls for subsequent experiments, and peel off the middle and outer scales (bulbs from 1st to 2nd floors from the outside to the inside) f...

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Abstract

The invention discloses a lilium oriental hybrid tissue culture seedling strengthening method which comprises the following steps: taking a squamous bulb of a lilium oriental hybrid aseptic seedling, and carrying out sprout induction culture on the squama in an induction culture medium; and after the sprouts are induced, inoculating the sprouts to a rooting and bulb-strengthening culture medium, and culturing to obtain a tissue culture seedling, wherein the rooting and bulb-strengthening culture medium comprises 4-6 g / L MS (Murashige-Skoog) powder, 60-70 g / L sucrose, 6-8 g / L agar and 5*10<-5>-5*10<-4> mmol / L brassinolide. The method can effectively promote the expansion of the squamous bulb of the tissue culture seedling, improves the quality of the squamous bulb, and is beneficial to obtaining the strong tissue culture seedling.

Description

technical field [0001] The invention belongs to the field of tissue culture, and in particular relates to a method for tissue-cultured seedlings of oriental lily. Background technique [0002] Lily (Lilium spp.) is known as the "king of flower bulbs" and is one of the top five fresh cut flowers in the world. In recent years, with the improvement of our country's national living standards, the consumption of lily has also increased year by year. Although my country is the main natural distribution center of wild lilies, the commercial cultivation of ornamental lilies started late, and the breeding and reproduction lagged behind seriously. For a long time, my country's cut flower lilies, especially the mainstream varieties of oriental hybrids 'Sorbonne' (L.oriental hybrids 'Sorbonne') have long relied on imports (mainly the Netherlands), and the cost of importing bulbs accounts for a very large part of the total production cost. High ratio. Therefore, localization of orienta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 吴昀夏宜平郭佳马怡迪张佳平杜方张琳
Owner CHENGBANG ECO ENVIRONMENT CO LTD
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