Shear type liquid core coupled surface plasma resonance imaging analyzer

Abstract

The invention discloses a shear type liquid core coupled surface plasma resonance imaging analyzer composed of a liquid core coupled surface plasma resonant unit, a surface plasma resonance imaging light excitation arm, a surface plasma resonance imaging light detection arm, a shear type two-arm linkage angle scanning mechanism, an electrical control system and an upper computer. When the shear type liquid core coupled surface plasma resonance imaging analyzer works, the position of a sensor chip is fixed, so that the light excitation angle can be scanned by only linking and rotating the surface plasma resonance imaging light excitation arm and the surface plasma resonance imaging light detection arm, an excited ray can be always vertical to an incident light window, and a reflected ray can be always vertical to an emitting light window, and furthermore, the defect of position offset of surface plasma resonance excitated light spots of the traditional instrument is overcome; and the shear type liquid core coupled surface plasma resonance imaging analyzer has the advantages such as no need of excited light angle correction, no need of binding oil, multi-interface reflection interference reduction and the like.

Description

technical field [0001] The invention belongs to the technical field of testing instruments, in particular to a surface plasmon resonance imaging analyzer. Background technique [0002] Surface plasmon resonance is a label-free, in situ and real-time interface analysis technique. The occurrence condition of surface plasmon resonance changes with the change of the refractive index of the sample on the sensing film-medium interface. Therefore, through surface plasmon resonance analysis, the refractive index and thickness information of the sample on the surface of the sensing film can be obtained, and the physical and chemical reactions on the surface of the sensing film can be monitored and analyzed. If the target molecule is bonded to the surface of the sensing film, Specific molecular interaction analysis can then be performed. Based on surface plasmon resonance technology, two types of analysis systems, surface plasmon resonance and surface plasmon resonance imaging, have...

AI Technical Summary

Problems solved by technology

[0004] 1. The shape of the prism is fixed, the excitation light cannot always be perpendicular to the incident surface of the prism, and the reflected light cannot always be perpendicular to the exit surface of the prism. When the light excitation angle changes, the position of the excitation spot will shift due to the light refraction effect , causing the light distribution to change, thus affecting the analysis effect; and it will also bring about the correction of the light excitation angle

[0005] 2. In order to reduce cost and facilitate use, the sensing film is mostly prepared on the substrate, and then pasted on the bottom surface of the prism with adhesive oil. The operation of adhesive oil is easy to generate air bubbles, which affects the imaging quality and analysis effect

[0006] 3. The incident surface of the excitation light and the prism, the reflected light and the exit surface of the prism cannot always be kept vertical, and the refractive index of the prism, adhesive oil and substrate is difficult to match ideally, which will bring reflection interference problems at multiple interfaces

[0007] All the above problems, in addition to the trouble of angle correction, will seriously affect the analysis quality of surface plasmon resonance and surface plasmon resonance imaging system

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