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Vaccine of encephalomyelitis of poultry

A technology for avian encephalomyelitis and avian brain, applied in the direction of antibody medical ingredients, medical preparations containing active ingredients, antiviral agents, etc., to achieve good commercial development prospects, effective immune protection, and genetically stable effects

Active Publication Date: 2014-04-09
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many countries in the world have used live vaccines to immunize most chicken flocks. Domestically, inactivated vaccines have been successfully developed, which can be used in areas where chicken production is started or the use of live vaccines is restricted. At present, there are no live vaccine products in China.

Method used

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  • Vaccine of encephalomyelitis of poultry
  • Vaccine of encephalomyelitis of poultry
  • Vaccine of encephalomyelitis of poultry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Breeding of avian encephalomyelitis virus strain

[0016] Take the brain, pancreas and duodenum of chicks with avian encephalomyelitis, grind them under sterile conditions, dilute them with sterilized normal saline at a ratio of 1:5, repeat freezing and thawing three times, centrifuge at 4000rpm for 30 minutes, and take the supernatant After filtering through the liquid filter, 10 1-day-old SPF chicks were inoculated in the brain, 0.1ml per chick. After symptoms such as ataxia and head and neck tremors appeared, blood was collected to kill them. Grind under sterile conditions, dilute 1:5 with sterilized saline, freeze-thaw 3 times, centrifuge at 4000rpm for 30 minutes, take the supernatant as the first-generation isolated virus (F1) and save it for later use. The F1 generation was inoculated into the brain of 1-day-old SPF chicks by the same method as above, and the second generation of isolated virus (F2) was prepared. This has been passed on for 4 gener...

Embodiment 2

[0027] Preparation and application of embodiment 2 poultry cerebrospinal live vaccine

[0028] 1 material

[0029] 1.1 Virus Seed The attenuated YBF02 strain isolated from CGMCC No. 8505 was selected as the avian encephalomyelitis virus used for vaccine production.

[0030] 1.2 Virus for inspection The virulent VR strain of avian encephalomyelitis virus for inspection is identified, preserved and supplied by the China Veterinary Drug Control Institute.

[0031] 1.3 Breeding eggs and test chickens

[0032] 1.3.1 SPF eggs were purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd.

[0033] 1.3.2 7-14-day-old SPF chickens SPF eggs were purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd., hatched by themselves after purchase, and reared in a negative pressure isolator after hatching.

[0034] 1.4 Other instruments and reagents for seedling production were provided by Qingdao Yibang Bioengineering Co., Ltd.

[0035] 2 methods

[0...

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Abstract

The invention provides vaccine of encephalomyelitis of poultry. The vaccine comprises antigen and protective agent, wherein the antigen comprises low virulent strain of encephalomyelitis of poultry with preservation number CGMCC No.8505. The vaccine prepared by the invention can effectively prevent encephalomyelitis of poultry; furthermore, the virus of the encephalomyelitis of poultry is taken as the antigen, is low virulent strain, is serially subcultured in a chicken body through horizontal transmission and infection, does not become strong again in toxicity, has stable heredity, and meets the standard that the attenuated vaccine of the encephalomyelitis of poultry does not become strong again in toxicity, so that the prepared vaccine can provide effective immune protection and has good commercialized development prospect.

Description

technical field [0001] The invention belongs to the technical field of poultry vaccine preparation, in particular to an avian encephalomyelitis vaccine. Background technique [0002] Avian encephalomyelitis (AE) is a viral infectious disease caused by avian encephalomyelitis virus (AEV), which mainly harms chicks under 4 weeks of age, and infects the central nervous system and causes non-suppurative encephalitis as the main pathological feature. Manifested by neurologic symptoms such as ataxia, head and neck tremors, and numbness of the hind digits, especially tremors in the head and neck. Hence, it used to be called "epidemic tremor". After chickens under 4 weeks of age are infected with AEV, the incidence rate is generally 20% to 60%, and the mortality rate is 25% on average, which can also exceed 50%. Adult chicken infection does not show neurological symptoms, but it can cause a transient decline in egg production. The decline range is 16% to 43%, and some are as high ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/125A61P31/14
Inventor 王红范根成杜元钊郭伟伟宋新宇胡潇
Owner YEBIO BIOENG OF QINGDAO
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