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Method for detecting genotoxic potential of heavy metal pollutants in soil

A technology of genotoxicity and heavy metals, applied in the field of genetic toxicology of environmental health, to achieve the effect of solving vulnerable

Inactive Publication Date: 2014-04-23
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, comet experiments on algae are rarely reported at home and abroad.

Method used

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  • Method for detecting genotoxic potential of heavy metal pollutants in soil
  • Method for detecting genotoxic potential of heavy metal pollutants in soil
  • Method for detecting genotoxic potential of heavy metal pollutants in soil

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Experimental program
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Embodiment 1

[0044] 1 Pretreatment of soil samples:

[0045] Soil samples were collected in Zijin Mountain as a clean soil control, and the sampling numbers in two places of a certain steel plant were No. 1 and No. 2 of a certain steel. Sampling The soil samples were collected according to the standard method of the National Environmental Protection Agency. The basic physical and chemical properties and heavy metal content of the soil samples are shown in Table 1.

[0046] Table 1 Basic physical and chemical properties and heavy metal content of soil samples

[0047]

[0048] 1) Values ​​are expressed as mean ± standard deviation (Mean ± S.D)

[0049] After air-drying, pass through a soil sieve with a diameter of 2 mm to remove plant residues, stones, etc., grind with an agate mortar, and pass through an 80-mesh sieve. After the soil is digested, warm the residue with 1ml of nitric acid to dissolve the residue, then transfer the solution to a 50ml volumetric flask, constant volume, sh...

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Abstract

The invention discloses a method for detecting genotoxic potential of heavy metal pollutants in soil. The method comprises the steps of 1, soil digestion; 2. preparation of microalgae cells; 3, glue spreading; 4. pyrolysis; 5. electrophoresis; 6. neutralization; 7. dyeing; and 8. DNA damage degree analysis and performing of ANOVA analysis by using statistic Origin 7.5 software. The comet image analysis is performed by adopting CASP software, the OTM (Olive Tail Moment) simultaneously reflects the DNA content and shape features in the comet in evaluation parameters and is a common index for quantifying the DNA damage degree. The method is an effective test method and can evaluate the genotoxic potential of heavy metals in the soil.

Description

technical field [0001] The invention belongs to the field of environmental health genetic toxicology, and in particular relates to a method for detecting the potential genetic toxicity of heavy metals in soil. Background technique [0002] Soil is an important natural resource for human survival and development and the basis for the existence of the entire terrestrial ecosystem, and it is also one of the important environmental elements. "Healthy" soils are important for sustainable agriculture and human survival. However, with the rapid development of my country's economy, the problem of soil pollution is becoming more and more serious. Serious soil pollution, especially heavy metal pollution, has become a common feature of many soils including urban soils. It is estimated that my country has 100,000 km 2 Soil is polluted, and highly toxic heavy metals such as As, Cd, Cr, Hg and Pb are seriously polluted in my country's soil pollution heavy metals. Studies have shown that...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/02C12R1/89
CPCG01N1/28G01N21/6486G01N27/447
Inventor 李梅崔益斌张刘俊嵇伏年李雅洁
Owner NANJING UNIV
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