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A kind of disinfection and sterilization method of dendrobium officinale capsule

A technology for disinfection and sterilization of Dendrobium candidum, applied in the biological field, can solve the problems of inability to sterilize Dendrobium candidum capsules, endanger human health, acute and chronic poisoning, etc., and achieve the effects of promoting early germination, no heavy metal residues, and reducing production costs

Active Publication Date: 2016-01-20
YUNNAN GAOSHAN BIOLOGICAL AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Conventional disinfection and sterilization methods mainly have the following defects: 1. It is impossible to sterilize the cracked Dendrobium officinale capsules
2. Mercuric chloride solution is highly toxic and can be absorbed by the human body through breathing and skin contact, which can easily cause acute and chronic poisoning and endanger human health
3. Mercury is a heavy metal, which is easy to cause heavy metal pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select healthy and full Dendrobium officinale capsules, remove the receptacles and residual petals; clean the selected capsules with washing liquid, dry them until the surface of the capsules is dry, soak them in 75% alcohol by weight for 30 seconds, and dry them. Standby; 4 hours before sowing, put the above-mentioned spare capsules into an airtight container, press 10g / m 3 Take the amount of sodium dichloroisocyanurate aerosol with a mass concentration of 2% and ignite it and put it into the airtight container for sterilization for 1 hour.

Embodiment 2

[0024] Select healthy and full Dendrobium officinale capsules, remove the receptacle and petal residues; clean the selected capsules with washing liquid, dry them until the surface of the capsules is dry, soak them in 75% alcohol by weight for 30 seconds, and dry them. Standby; 4 hours before sowing, put the above-mentioned spare capsules into an airtight container, press 8g / m 3 Take the amount of sodium dichloroisocyanurate aerosol with a mass concentration of 2% and ignite it and put it into the airtight container for sterilization for 1 hour.

Embodiment 3

[0026] Select healthy and full Dendrobium officinale capsules, remove the receptacle and petal residues; clean the selected capsules with washing liquid, dry them until the surface of the capsules is dry, soak them in 75% alcohol by weight for 30 seconds, and dry them. Standby; 4 hours before sowing, put the above-mentioned spare capsules into an airtight container, press 12g / m 3 Take the amount of sodium dichloroisocyanurate aerosol with a mass concentration of 2% and ignite it and put it into the airtight container for sterilization for 1 hour.

[0027] Using the capsules of Dendrobium candidum sterilized by the above-mentioned embodiments, after taking out the seeds of Dendrobium candidum for direct sowing, the seeds turn green in 5-6 days, and the germination rate reaches about 98%.

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Abstract

The invention relates to a method for sterilizing and disinfecting Dendrobium officinale capsules, belonging to the field of biotechnology. The method comprises the steps of firstly, selecting healthy and fully grown Dendrobium officinale capsules, removing tori and residual petals; secondly, cleaning the selected Dendrobium officinale capsules with scrubbing solution, airing until the surfaces of the capsules have no moisture, soaking with alcohol with weight percentage of 75%, wiping and setting aside; thirdly, placing the treated capsules into a closed vessel 4 hours before sowing, and igniting dichlord isocyanurice acid aerosol with mass concentration of 2% at 8-12g / m3 and placing into the closed vessel for sterilization. The method has the beneficial effects of having good sterilizing and disinfecting effect and no harm to health of human body and promoting Dendrobium officinale seed germination in advance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for disinfecting and sterilizing dendrobium officinale capsules. Background technique [0002] Under natural conditions, the fruit setting rate of Dendrobium officinale is extremely low, so artificial pollination is required to implement cross-pollination to increase the fruit setting rate, so as to meet the needs of industrial seedling cultivation. After pollination, the capsules can be harvested after the fruit turns from green to yellow and the seeds disperse. [0003] There are tens of thousands of seeds in a capsule of Dendrobium officinale. Due to the lack of endosperm in the seeds, the seeds of Dendrobium officinale cannot germinate in the natural environment. Therefore, industrial production of seedlings is carried out by artificial pollination combined with vegetative propagation. The seeds of Dendrobium officinale are sprinkled on suitable medium and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/08
Inventor 朱明旺
Owner YUNNAN GAOSHAN BIOLOGICAL AGRI
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