A method for callus induction and proliferation of water lily mature embryos

Abstract

The invention discloses a method for inducing and multiplying callus of mature embryos of water lily. The specific steps include: material preparation, pretreatment and disinfection of mature embryos, induction of embryogenic callus and proliferation of callus. The invention uses the mature embryo of the water lily as the explant, the material is convenient to obtain, the pollution rate is low after the disinfection treatment, the callus induction rate is high, and the proliferation effect is good, and the invention can be used for genetic transformation, germplasm resource preservation and rapid propagation production, and regeneration of water lilies. System and transgenic research to establish a foundation.

Description

technical field

[0001] The invention relates to a plant tissue culture technology, in particular to a method for inducing and multiplying callus of mature embryos of water lily. Background technique

[0002] Nymphaea (Nymphaea) is a perennial floating-leaf aquatic herb of Nymphaeaceae (Nymphaeaceae), mainly distributed in temperate and tropical zones. In the garden waterscape (Liao Weiwei, 2016), the market demand is broad. At present, the vast majority of water lily varieties are produced by artificial hybridization and natural hybridization. The breeding cycle is long and the randomness is strong (Li Shujuan, 2019). Seedlings are produced by tissue culture, which has a fast production speed and can shorten the breeding cycle. , Effectively maintain the excellent traits of seedlings and preserve rare water lily germplasm resources. Therefore, rapid propagation through tissue culture is an effective way to meet application requirements as soon as possible. It is imminent to...

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