Method for Quantitative Detection of Banana Fusarium wilt Race 4 from Soil

Abstract

The invention discloses a quantitative detection method for FOC (Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyd. & Hans.) race 4 from the soil. The method comprises the following steps: pretreating a sample to be detected and extracting DNA of the sample to be detected; preparing specific primers; conducting fluorescent nucleic acid isothermal amplification detection technique RealAmp reaction; constructing a RealAmp standard curve; and determining the result. The real-time fluorescent nucleic acid isothermal amplification detection technology provided by the invention is a novel nucleic acid detection technology combining a new generation of isothermal nucleic acid amplification technology with the real-time fluorescent detection technology, and has the advantages of high sensitivity, high specificity, low pollution and stable reaction. RealAmp can quantitatively detect pathogens; because of the characteristics of LAMP, Bst DNA polymerase has chain exchange capacity and good tolerance of inhibiting substances contained in the soil DNA extracts; the method carries out detection work on the soil, avoids cover opening and influence by contaminants and conducts on-site soil sample detection; the method for analysis and determination of reaction products is very simple; therefore, the method is suitable for wide application.

Description

technical field [0001] The invention belongs to the field of banana fusarium wilt detection, in particular to a method for quantitatively detecting race 4 of banana fusarium wilt from soil. Background technique [0002] Banana is a plant of the genus Musa of the Musaceae family, and is an important economic crop and food crop in tropical and subtropical regions. Banana Fusarium Wilt is a devastating disease of the banana industry, which seriously restricts the healthy development of my country's banana industry. [0003] The pathogen of banana wilt is FusariumoxysporumSchlevht.f.sp.cubense(E.F.Smith)Snyd.&Hans.(Foc), formerly known as FusariumcubenseE.F.Smith, belongs to half-knowledge fungi, Tumoraceae, Fusarium genus, Chinese The name is Fusarium oxysporum Cuban specialization. The fungus is divided into 3 races. In my country, there are mainly race 1 (race1, R1) and race 4 (race4, R4). Race No. 1 has a long history and mainly infects bananas, etc. The harm of race No....

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Problems solved by technology

[0014] The purpose of the embodiments of the present invention is to provide a method for quantitatively detecting the No. 4 race of Fusarium wilt of banana from soil, aiming to solve the problems of low sensitivity, low specificity, serious pollution, and poor reaction stability of the previous first-generation LAMP detection method. question

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