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Large-scale creation method of wheat allos translocation line

A translocation line, wheat technology, applied in biochemical equipment and methods, botanical equipment and methods, microbial determination/inspection, etc., can solve the problem of cumbersome steps, time-consuming and laborious, and not suitable for large-scale identification of exogenous genetic material. and other problems, to achieve the effect of improving utilization efficiency, enhancing accuracy, and improving advanced performance.

Active Publication Date: 2014-05-21
渤海粮仓南皮种业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the indirect method GISH can amplify the hybridization signal cascade and enhance the sensitivity, it is not suitable for the large-scale identification of exogenous genetic material in wheat chromosome engineering breeding due to the cumbersome steps and time-consuming and laborious steps.

Method used

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  • Large-scale creation method of wheat allos translocation line
  • Large-scale creation method of wheat allos translocation line
  • Large-scale creation method of wheat allos translocation line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1, large-scale creation of heterologous translocation lines in wheat

[0057] 1. Large-scale creation of translocation systems

[0058] Will F 1 The offspring materials were used as exogenous genome donors to obtain a large number of abundant chromosome structural variations of Echinopsis elongatum through pollen radiation, and then these structural variations were introduced into the background of "Xiaoyan 81" through conventional hybridization and continuous backcrossing. Large-scale creation of heterogeneous new germplasm of wheat.

[0059] 1. Cross "Changwu 131" (female parent; 42 wheat chromosomes) with "Xiaoyan 68" (male parent; 42 wheat chromosomes and 14 exogenous chromosomes) to obtain seeds (seeds have 42 wheat chromosome and 7 exogenous chromosomes).

[0060] 2. Cultivate the seeds obtained in step 1 into plants (F 1 Substitute plant), take F 1 For generation plants, the wheat ears of the plants that are about to bloom in large quantities are cut...

Embodiment 2

[0109] Example 2, large-scale creation of wheat heterologous translocation lines

[0110] 1. Large-scale creation of translocation systems

[0111] 1. Cross "Shi 4185" (as the female parent; containing 42 wheat chromosomes) with "Xiaoyan 784" (as the male parent; containing 42 wheat chromosomes and 14 foreign chromosomes) to obtain seeds (the seeds have 42 wheat chromosomes chromosome and 7 foreign chromosomes).

[0112] 2. Cultivate the seeds obtained in step 1 into plants (F 1 Substitute plant), take F 1 For generation plants, the wheat ears of the plants that are about to bloom in large quantities are cut, and the structural variation of chromosomes is induced by 60Co-γ ray radiation (the total radiation absorbed dose is 15Gy, and the dose rate is 4.7Gy / min), and fresh wheat ears are collected immediately after irradiation. For pollen, pollinate the ears of "Xiaoyan 81" that had been emasculated 3-4 days ago, and isolate them by bagging, and the obtained seeds are M 0 g...

Embodiment 3

[0128] Example 3, large-scale creation of wheat heterologous translocation lines

[0129] 1. Large-scale creation of translocation systems

[0130] 1. Cross "Jing 411" (as the female parent; containing 42 wheat chromosomes) and "Xiaoyan 7430" (as the male parent; containing 42 wheat chromosomes and 14 exogenous chromosomes) to obtain seeds (the seeds have 42 wheat chromosomes chromosome and 7 foreign chromosomes).

[0131] 2. Cultivate the seeds obtained in step 1 into plants (F 1 Substitute plant), take F 1 For generation plants, the wheat ears of the plants that are about to bloom in large quantities are cut, and the structural variation of chromosomes is induced by 60Co-γ ray radiation (the total radiation absorbed dose is 15Gy, and the dose rate is 4.7Gy / min), and fresh wheat ears are collected immediately after irradiation. For pollen, pollinate the ears of "Xiaoyan 81" that had been emasculated 3-4 days ago, and isolate them by bagging, and the obtained seeds are M 0...

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Abstract

The invention discloses a large-scale creation method of a wheat allos translocation line. The method comprises the following steps: (1) hybridizing common wheat and partial allodidiploid to obtain seeds; (2) cultivating the seeds into F1-generation plants; (3) radiating the pollen of the F1-generation plants with 60Co-gamma rays, and pollinating the castrated common wheat to obtain seeds; (4) performing in-situ hybridization of the seeds and classifying into a translocation line, an alien addition line containing a few exogenous chromosomes and an alien addition line containing multiple exogenous chromosomes; performing the steps (3) and (4) on the alien addition line containing a few exogenous chromosomes instead of the F1-generation plants, and repeating in turn; and performing the steps (1), (2), (3) and (4) on the alien addition line containing multiple exogenous chromosomes instead of the partial allodidiploid, and repeating in turn. The large-scale creation of the wheat allos translocation line improves the utilization efficiency of intermediate materials, enhances the creation accuracy of the translocation line and is advanced, innovative and practical.

Description

technical field [0001] The invention relates to a method for large-scale creation of heterologous translocation lines of wheat. Background technique [0002] The heterologous species of wheat contain many excellent genes related to high yield, high quality, stress resistance and other traits. The development and utilization of these genetic resources and the introduction of heterologous DNA into wheat cultivars to enrich the germplasm resources of wheat have always been one of the important topics in wheat genetics and breeding, and it is also the only way for future wheat improvement. The transfer of exogenous DNA to the genetic background of wheat through chromosome engineering has opened up a new situation in wheat genetics and breeding. The basic procedure for creating usable germplasm materials in breeding is: interspecies hybridization → F1, allo-amphidiploid or partial allo-amphidiploid → allo-additional line → allo-substitution line → translocation line. Among them...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H1/06C12Q1/68
Inventor 郑琪李宏伟李滨李振声
Owner 渤海粮仓南皮种业有限公司
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