Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

QuEChERS extraction method for poisonous substance extraction and application of QuEChERS extraction method

An extraction method and technology for toxic substances, which are applied in the fields of QuEChERS extraction, rapid screening of food poisoning, and extraction of unknown toxic substances in food poisoning samples, can solve problems of different properties, and achieve the effect of being beneficial to timely treatment and criminal investigation work.

Inactive Publication Date: 2014-05-28
BEIJING CENT FOR DISEASE PREVENTION & CONTROL
View PDF4 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, this method is mainly used for the analysis of pesticide residues in fruits and vegetables, and a small part of it is also used in the analysis of quinolones, sulfonamides, benzimidazoles, and avermectins in animal food matrices such as milk, muscle, eggs, and liver. Veterinary drug residue detection, but due to the variety and nature of veterinary drugs, it brings certain challenges to the analysis and detection of multiple residues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • QuEChERS extraction method for poisonous substance extraction and application of QuEChERS extraction method
  • QuEChERS extraction method for poisonous substance extraction and application of QuEChERS extraction method
  • QuEChERS extraction method for poisonous substance extraction and application of QuEChERS extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The recovery test of embodiment 1 five kinds of different toxic substances in nine kinds of matrices

[0022] Take five parts of the nine samples of milk, liver, pork, eggs, fruits, vegetables, gastric juice, blood, and urine, and add toxic substances orbifloxacin, lomefloxacin, Flufloxacin, ofloxacin, dafloxacin, the above samples are extracted with the QuEChERS extraction method of the present invention respectively, and are detected and analyzed by liquid chromatography-time-of-flight mass spectrometry, and the recovery rate is shown in the following table 2 , in which milk, liver, pork, and eggs are all commercially available samples; fruit is a mixture of apples, pears, oranges, grapes, and dragon fruit; vegetables are a mixture of cabbage, rapeseed, radish, and artemisia stalks; Proportion is: 2g sodium chloride, 3.2g pepsin, 7mL36.5% hydrochloric acid is added water to 1000mL; figure 1Shown, in addition to the urine matrix, recovery of orbifloxacin, lomefloxacin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a QuEChERS extraction method for poisonous substance extraction, which is especially suitable for extracting unknown poisonous substances in food poisoning samples. The scheme is that the extraction method comprises the following steps: at first, extracting homogenized or mixed samples with acidiferous acetonitrile or Na2 EDTA to obtain extract liquor; adding NaC1 and Na2SO4 in the extract liquor, and mixing to obtain mixed liquor; carrying out supersonic centrifuge on the mixed liquor to obtain supernate, enabling the supernate to be subjected to C18 and anhydrous sodium sulfate purification to obtain an extracting solution, and then detecting and analyzing the extracting solution. The extraction method and application are suitable for various different matrixes: pork, pork liver, milk, eggs, fruits, vegetables, gastric juice, blood and urine, are high in recovery rate and quick and reliable to test, are used for quickly screening the actual poisoning samples, and facilitate the timely treatment and criminal investigation on poisoned personnel.

Description

technical field [0001] The invention relates to a sample pretreatment method and its application, in particular to a QuEChERS extraction method for extracting toxic substances, which is especially suitable for the extraction of unknown poisons in food poisoning samples, and realizes rapid screening of food poisoning. Background technique [0002] QuEChERS (abbreviation for Quick, Easy, Cheap, Effective, Rugged and Safe) method is a rapid pretreatment method developed in recent years, which can conveniently, economically and quickly extract target compounds from food matrices. The most original QuEChERS method has no buffer salt, only MgSO4 and NaCl. Currently, the internationally recommended QuEChERS methods are: the EU EN15662 method and the usage in AOAC. The EN15662 and AOAC official 2007.01 methods have improved the original method. In the extraction step Buffer salt is added, which can expand the range of target compounds. These two methods are mainly aimed at fruits an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/28
Inventor 邵兵郭巧珍高馥蝶张晶李敏
Owner BEIJING CENT FOR DISEASE PREVENTION & CONTROL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com