QuEChERS extraction method for poisonous substance extraction and application of QuEChERS extraction method

An extraction method and technology for toxic substances, which are applied in the fields of QuEChERS extraction, rapid screening of food poisoning, and extraction of unknown toxic substances in food poisoning samples, can solve problems of different properties, and achieve the effect of being beneficial to timely treatment and criminal investigation work.

Inactive Publication Date: 2014-05-28
BEIJING CENT FOR DISEASE PREVENTION & CONTROL
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Problems solved by technology

[0004] At present, this method is mainly used for the analysis of pesticide residues in fruits and vegetables, and a small part of it is also used in the analysis of quinolones, sulfonamides, benzimidazoles, and avermectins i...

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  • QuEChERS extraction method for poisonous substance extraction and application of QuEChERS extraction method
  • QuEChERS extraction method for poisonous substance extraction and application of QuEChERS extraction method
  • QuEChERS extraction method for poisonous substance extraction and application of QuEChERS extraction method

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Embodiment 1

[0021] The recovery test of embodiment 1 five kinds of different toxic substances in nine kinds of matrices

[0022] Take five parts of the nine samples of milk, liver, pork, eggs, fruits, vegetables, gastric juice, blood, and urine, and add toxic substances orbifloxacin, lomefloxacin, Flufloxacin, ofloxacin, dafloxacin, the above samples are extracted with the QuEChERS extraction method of the present invention respectively, and are detected and analyzed by liquid chromatography-time-of-flight mass spectrometry, and the recovery rate is shown in the following table 2 , in which milk, liver, pork, and eggs are all commercially available samples; fruit is a mixture of apples, pears, oranges, grapes, and dragon fruit; vegetables are a mixture of cabbage, rapeseed, radish, and artemisia stalks; Proportion is: 2g sodium chloride, 3.2g pepsin, 7mL36.5% hydrochloric acid is added water to 1000mL; figure 1Shown, in addition to the urine matrix, recovery of orbifloxacin, lomefloxacin...

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Abstract

The invention relates to a QuEChERS extraction method for poisonous substance extraction, which is especially suitable for extracting unknown poisonous substances in food poisoning samples. The scheme is that the extraction method comprises the following steps: at first, extracting homogenized or mixed samples with acidiferous acetonitrile or Na2 EDTA to obtain extract liquor; adding NaC1 and Na2SO4 in the extract liquor, and mixing to obtain mixed liquor; carrying out supersonic centrifuge on the mixed liquor to obtain supernate, enabling the supernate to be subjected to C18 and anhydrous sodium sulfate purification to obtain an extracting solution, and then detecting and analyzing the extracting solution. The extraction method and application are suitable for various different matrixes: pork, pork liver, milk, eggs, fruits, vegetables, gastric juice, blood and urine, are high in recovery rate and quick and reliable to test, are used for quickly screening the actual poisoning samples, and facilitate the timely treatment and criminal investigation on poisoned personnel.

Description

technical field [0001] The invention relates to a sample pretreatment method and its application, in particular to a QuEChERS extraction method for extracting toxic substances, which is especially suitable for the extraction of unknown poisons in food poisoning samples, and realizes rapid screening of food poisoning. Background technique [0002] QuEChERS (abbreviation for Quick, Easy, Cheap, Effective, Rugged and Safe) method is a rapid pretreatment method developed in recent years, which can conveniently, economically and quickly extract target compounds from food matrices. The most original QuEChERS method has no buffer salt, only MgSO4 and NaCl. Currently, the internationally recommended QuEChERS methods are: the EU EN15662 method and the usage in AOAC. The EN15662 and AOAC official 2007.01 methods have improved the original method. In the extraction step Buffer salt is added, which can expand the range of target compounds. These two methods are mainly aimed at fruits an...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 邵兵郭巧珍高馥蝶张晶李敏
Owner BEIJING CENT FOR DISEASE PREVENTION & CONTROL
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