Compound for treatment of cancer

A compound, cancer technology, applied in the field of medicine, can solve the problems of poor selectivity of combined chemotherapeutic drugs, enhance the pro-apoptotic necrosis effect, increase the anti-cancer effect of chemotherapy drugs, etc. The effect of increasing the killing, increasing the anti-cancer effect

Inactive Publication Date: 2014-06-18
HUAZHONG UNIV OF SCI & TECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0008] The task of the present invention is to provide a compound for treating cancer, which can increase the anticancer effect of chemotherapeutic drugs, enhance the effect of chemotherapeutic drugs on cancer cells and promote apoptosis and necrosis, and will not increase the effect of chemotherapeutic drugs on normal cells. In order to solve the above-mentioned problem of poor selectivity of combined chemotherapy drugs,

Method used

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  • Compound for treatment of cancer
  • Compound for treatment of cancer
  • Compound for treatment of cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Proliferation rate of HepG2 cells and LO2 cells in different calcium ion groups

[0026] 1. Culture LO2 cells and HepG2 cells with DMEM medium containing 10% calf serum, and place the cells in 5% CO after passage 2 cultured in a 37°C incubator.

[0027] 2. When the cells are in the logarithmic growth phase, add 500ul of trypsin (to detach the cells from the bottle) to each bottle, shake the bottle to make the trypsin fully contact with the cells, and after 2 minutes, add 500ul of calf serum to the bottle to stop The role of pancreatic enzymes.

[0028] 3. Add 3ml of medium to the bottle, blow off the cells from the bottle with a pipette, transfer the cell suspension into a centrifuge tube, and centrifuge at 600g for 5min.

[0029] 4. Pour off the supernatant, add 3ml of fresh medium, and pipette carefully to disperse the cells.

[0030] 5. Count to 5 x 10 per well 3 The cells were planted in a 96-well plate, and the culture medium (containing 10% calf seru...

Embodiment 2

[0035] Example 2 Effects of compound drug (weight ratio of calcium chloride to doxorubicin: 10000:1) on liver cancer cells HepG2 and normal liver cells LO2

[0036] (1) Effects of compound drug (weight ratio of calcium chloride and doxorubicin: 10000:1) on the proliferation of liver cancer cells HepG2 and normal liver cells LO2

[0037] 1. Culture LO2 cells and HepG2 cells with DMEM medium containing 10% calf serum, and place the cells in 5% CO after passage 2 cultured in a 37°C incubator.

[0038] 2. When the cells are in the logarithmic growth phase, add 500ul of trypsin (to detach the cells from the bottle) to each bottle, shake the bottle to make the trypsin fully contact with the cells, and after 2 minutes, add 500ul of calf serum to the bottle to stop The role of pancreatic enzymes.

[0039] 3. Add 3ml of medium to the bottle, blow off the cells from the bottle with a pipette, transfer the cell suspension into a centrifuge tube, and centrifuge at 600g for 5min.

[004...

Embodiment 3

[0092] Example 3 Effects of compound drugs (the weight ratio of calcium chloride to doxorubicin is 5000:1) on the proliferation of liver cancer cells HepG2 and normal liver cells LO2

[0093] In order to uniformly observe the curative effect of the drug after adjusting the ratio, the final concentration of ADM is still fixed at 0.1ug / ml in this embodiment, that is, the concentration of the compound drug is 0.5mg / mL. The experimental operation is the same as that of Example 2 (1).

[0094] The results are shown in Table 1: in LO2 cells, compared with adriamycin, the inhibitory effect on cell proliferation in the compound drug group had no significant change (P>0.05); but in HepG2 cells, compared with adriamycin, the cell proliferation in the compound drug group The inhibitory effect increased slightly (P<0.05).

[0095] Table 1 Extracellular calcium ions enhance the effect of doxorubicin on the proliferation rate of HepG2 cells and LO2 cells (n=3, %)

[0096]

[0097] ...

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Abstract

The invention provides a compound for treatment of cancer, the compound is prepared by mixing a calcium salt and chemotherapy drug doxorubicin, the weight ratio of calcium salt to doxorubicin is 5000:1-20000:1, preferably is 10000:1, and the calcium salt can be calcium chloride. The compound medicine can increase the anticancer effect of the chemotherapy drug, and increases the inhibitory and apoptosis-promoting necrosis effect of the chemotherapy drug on cancer cells, and does not increase killing and wounding of the chemotherapy drug on normal cells.

Description

technical field [0001] The invention belongs to the field of medicine and relates to medicines for treating cancer. Background technique [0002] Liver cancer has a high incidence, rapid progression, and high degree of malignancy [1] , is one of the most common malignant tumors in the world, and its incidence ranks fifth in the world [2，3] . At present in China, liver cancer is the second most common malignant tumor and the second leading cause of cancer death. There are about 360,000 new cancer patients every year, and about 350,000 patients die of liver cancer every year, which seriously threatens people's health. [4] . According to the statistics of the World Health Organization in 2000, there are about 560,000 new cases of liver cancer every year in the world, most of which are Asian patients, and the number of cases in my country accounts for more than 2 / 3 of the total number in Asia, which is a high incidence area of ​​liver cancer. If liver cancer patients are not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/14A61P35/00A61K31/704
Inventor 吴志刚谢虹黄雪雪刘延一
Owner HUAZHONG UNIV OF SCI & TECH
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