Preparation and application of star-shaped bioactive filler for immobilization of sulfate-reducing bacteria based on mesh carrier

A bioactive, sulfate technology, applied in the direction of immobilization on/in organic carriers, biological water/sewage treatment, anaerobic digestion treatment, etc. The buried body is easy to fall off and other problems, so as to improve the hydrophilicity, the biomass of high sulfate-reducing bacteria, and the overall stability.

Active Publication Date: 2016-01-13
湖南汨罗循环经济产业园区科技创新服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After experimental research and comparison, we found that the bioactive fillers prepared based on the traditional filler structure and embedding method were difficult to achieve the desired effect in the test application. and many other issues

Method used

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  • Preparation and application of star-shaped bioactive filler for immobilization of sulfate-reducing bacteria based on mesh carrier
  • Preparation and application of star-shaped bioactive filler for immobilization of sulfate-reducing bacteria based on mesh carrier
  • Preparation and application of star-shaped bioactive filler for immobilization of sulfate-reducing bacteria based on mesh carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of Mesh Support

[0033] Using high molecular polymers such as polypropylene as the main material, add 6wt% polyvinyl alcohol (PVA) to make a sheet-shaped mesh carrier with a length of 1.0cm and a width of 0.7cm. The shape of the mesh is square, the side length is 2mm, and the wire diameter is 1.5mm. Six pieces of the above-mentioned sheet-shaped carriers are melted and bonded to form a star-shaped mesh carrier with an included angle of 60 degrees.

[0034] 2. Preparation of Sulfate Reducing Bacteria Concentrate

[0035] The suspension of sulfate-reducing bacteria after amplification culture was centrifuged and concentrated to obtain 3.5×10 9 A concentrate of sulfate-reducing bacteria per mL.

[0036] 3. Preparation of embedding solution

[0037]Weigh 5kg of polyvinyl alcohol, add 25L of ultrapure water, heat to 85°C, and dissolve for 15min. Take out the dissolved polyvinyl alcohol solution and stir it evenly, then heat it for 5 minutes, stir it even...

Embodiment 2

[0045] 1. Preparation of Mesh Support

[0046] Using high molecular polymers such as polyethylene as the main material, add 5wt% polyvinyl alcohol (PVA) to make a sheet-shaped mesh carrier with a length of 1.0cm and a width of 0.7cm. The shape of the mesh is square, the side length is 2mm, and the wire diameter is 1.5mm. Six pieces of the above-mentioned sheet-shaped carriers are melted and bonded to form a star-shaped mesh carrier with an included angle of 60 degrees.

[0047] 2. Preparation of Sulfate Reducing Bacteria Concentrate

[0048] The suspension of sulfate-reducing bacteria after amplification culture was centrifuged and concentrated to obtain 7.5×10 9 A concentrate of sulfate-reducing bacteria per mL.

[0049] 3. Preparation of embedding solution

[0050] Weigh 5kg of polyvinyl alcohol, add 25L of ultrapure water, heat to 85°C, and dissolve for 15min. Take out the dissolved polyvinyl alcohol solution and stir it evenly, then heat it for 5 minutes, stir it even...

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Abstract

The invention discloses preparation and an application of a sulfate reducing bacteria immobilized star-shaped bioactive filler based on a reticulate carrier, and belongs to the technical field of water treatment. The star-shaped bioactive filler comprises two parts: an embedding body and the carrier; the carrier is a reticulate star-shaped carrier formed by taking polyethylene or polypropylene and the like as a main material, adding polyvinyl alcohol or other hydrophilic materials and carrying out hot melting or plate hot pressing; with a reticulate structure of the carrier, the embedding body can be allowed to penetrate through meshes to form a riveting fixing structure together with the carrier so as to increase the filler overall stability; an embedding liquid is formed by mixing a sulfate reducing bacteria concentrated solution and a polyvinyl alcohol solution; the reticulate carrier is evenly coated with the embedding liquid, and the embedding body is formed after boric acid secondary cross-linking immobilization of the embedding liquid and is combined with the reticulate carrier to obtain the sulfate reducing bacteria bioactive filler. The prepared bioactive filler not only can effectively solve the problems that sulfate reducing bacteria are poor in adhesion ability, poor in film-forming ability and the like, but also can improve the processing ability of a reactor and shorten the start-up time.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to the preparation and application of star-shaped bioactive fillers immobilized with sulfate-reducing bacteria based on a mesh carrier. Background technique [0002] In the process of using sulfate-reducing bacteria (Sulfate-Reducing Bacteria, SRB) to treat sulfate wastewater, the number, metabolic activity and ecological factors of functional microorganisms (mainly referring to sulfate-reducing bacteria) directly affect the operation and treatment efficiency of the reactor. . In the anaerobic treatment of sulfate wastewater, there are abundant carbon sources (COD originally in the wastewater or added for treatment) and suitable anaerobic conditions, many microorganisms with similar habitat conditions can gather, so how to maintain SRB The dominant position of the population, suitable ecological factors and substrate competitive advantages are the research focus of SRB me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08C02F3/34C02F3/00C02F3/28
Inventor 杨宏胡希佳陈伟管清坤王猛鄢琳王小乐吴城锋孟婷陶慕翔尚海源赵月兰王玉洁姚仁达
Owner 湖南汨罗循环经济产业园区科技创新服务中心
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