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Rotavirus-adenovirus-occult blood combined-measuring kit

A technology of rotavirus and adenovirus, which is applied in the direction of biological testing, material inspection products, measuring devices, etc., can solve the problems that rotavirus has not yet been discovered, and achieve the goal of being suitable for large-scale promotion and application, with a long course of disease and avoiding inaccurate test results. accurate effect

Inactive Publication Date: 2014-08-06
无锡博慧斯生物医药科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are colloidal gold test strips for rotavirus, adenovirus and occult blood, or colloidal gold test strips for joint detection of rotavirus and adenovirus, which greatly facilitates clinical diagnosis; but no rotavirus, adenovirus and occult blood have been found Colloidal gold test strip products or reports of the three indicators joint inspection

Method used

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  • Rotavirus-adenovirus-occult blood combined-measuring kit
  • Rotavirus-adenovirus-occult blood combined-measuring kit
  • Rotavirus-adenovirus-occult blood combined-measuring kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Colloidal gold labeling

[0044] Antibody (anti-human rotavirus antibody) colloidal gold labeling method for rotavirus detection is as follows: use potassium carbonate solution to adjust the pH value of 50ml colloidal gold solution to 7.7; Add the anti-rotavirus antibody to be labeled into the above colloidal gold solution, mix well, and let it stand at room temperature for 30 minutes; slowly add 12.5ml of blocking solution to the above colloidal gold solution, mix well, and let it stand at room temperature for 30 minutes; Clear, leave loose precipitate; Dissolve the precipitate in each tube with 25ml washing solution, centrifuge at 6000g4℃ for 1h, remove the supernatant, leave loose precipitate; dissolve the precipitate in each tube with 1.2ml washing solution, transfer to a 1.5ml centrifuge tube, centrifuge at 4500g4℃ for 1h , remove the supernatant; all pellets were resuspended with 1ml colloidal gold antibody resuspension solution and stored at 4°C.

[00...

Embodiment 2

[0046] Spray point of embodiment 2 colloidal gold pad

[0047] Antibody (anti-human rotavirus antibody) colloidal gold pads for rotavirus diagnosis are sprayed as follows: Colloidal gold antibody solution dilution: Dilute the colloidal gold antibody conjugate prepared above by 4 times with colloidal gold antibody resuspension ;Set the dot film instrument, turn on the power supply of the dot film instrument, set the spray point program, the spray point volume is 8ul / cm; the No. 1 pipeline is the spray point channel; the film dot instrument initialization: put the No. 1 pipeline in the colloidal gold resistance In the body weight suspension solution, select the initialization program and initialize 6 cycles; Spray point: place the gold standard pad on the spotting device at a fixed position, press the "GO" key on the control panel to start spraying, remove it after pointing, Check the well-sprayed gold label pad. The sprayed colloidal gold antibody strips are uniform, continuous...

Embodiment 3

[0049] The preparation of embodiment 3 chromatographic membranes

[0050] The antibody (anti-human rotavirus antibody) chromatographic membrane preparation method for rotavirus diagnosis is as follows: take 500ug of mouse anti-human rotavirus antibody, add it to a 5ml graduated centrifuge tube, dilute the antibody to 1ml, and mark the container T sign. Take 25ul of goat anti-mouse IgG antibody, add it to a 5ml graduated centrifuge tube, dilute the antibody to 1ml, and mark the container with a C mark. Set the film spotting instrument, turn on the power supply of the film spotting instrument, set the spray point program, and the spray point volume is 1ul / cm; the No. 1 pipeline is the detection channel with spray points, and the No. 2 pipeline is the control channel with spray point points; the film spotting instrument Initialization: put the No. 1 pipe in the detection zone solution, put the No. 2 pipe in the control zone solution, select the initialization program, and initia...

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Abstract

The invention relates to a rotavirus-adenovirus-occult blood combined-measuring kit. The rotavirus-adenovirus-occult blood combined-measuring kit comprises a rotavirus-adenovirus-occult blood immunochromatographic assay test strips, or comprises a rotavirus immunochromatographic assay test strip, an adenovirus immunochromatographic assay test strip and a occult blood immunochromatographic assay test strip, or comprises a rotavirus immunochromatographic assay test strip and an adenovirus-occult blood immunochromatographic assay test strip, or comprises a rotavirus-adenovirus immunochromatographic assay test strip and an occult blood immunochromatographic assay test strip, or comprises a rotavirus-occult blood immunochromatographic assay test strip and an adenovirus immunochromatographic assay test strip. Preferably, the immunochromatographic assay test strip binding pad and chromatography film are respectively coated with corresponding immunolabelling antibodies and detection antibodies. Through one sampling process, the rotavirus-adenovirus-occult blood combined-measuring kit can simultaneously detect rotaviruses, adenoviruses and occult blood, can be used simply, can provide a combined-measuring result as a forceful basis for next-step accurate treatment and is suitable for large-scale popularization application.

Description

technical field [0001] The invention relates to the technical field of detection kits, in particular to the technical field of joint detection kits, in particular to a joint detection kit for rotavirus, adenovirus and occult blood. Background technique [0002] Infectious diarrheal disease is a group of intestinal infectious diseases that are widely distributed and prevalent all over the world. It has a high incidence and rapid transmission, and is especially harmful to children's health. Among them, bacterial diarrhea and viruses (mainly including rotavirus and adenovirus) infectious diarrhea. [0003] For infectious diarrhea in children, it is generally necessary to make a differential diagnosis of the pathogen in clinical practice in order to make appropriate treatment. Commonly used detection methods at present, such as stool routine, culture and immunological detection; stool routine generally includes indicators such as stool color, shape, fecal cells, occult blood, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/72G01N33/68G01N33/558
CPCG01N33/56983G01N33/558G01N2333/075G01N2333/14
Inventor 韦彦余时振华华一民赵静贾梦玥
Owner 无锡博慧斯生物医药科技有限公司
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