A method for inducing rooting and hardening of potato virus-free tissue-cultured seedlings

A technology for tissue culture seedlings and potatoes, which is applied in the directions of botanical equipment and methods, cultivation, soilless culture, etc., can solve the growing environmental conditions of tissue culture seedlings, such as poor regulation of temperature and humidity, nutrient solution concentration and pH, and large bacterial diseases. Due to the large area of ​​spread and the difficulty of disinfection, the effects of controlling the spread of bacterial diseases, strong adaptability and simple operation are achieved.

Active Publication Date: 2016-04-13
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The shortcoming of prior art is: 1, potato tissue culture seedling is still not robust enough after seedling hardening, especially root system is underdeveloped; 2, cost is high, and operation is more complicated; High risk; 4. It is difficult to thoroughly disinfect substrates such as perlite, vermiculite, humus, etc., and there is a high risk of disease after repeated use; 5. The growth environment conditions of tissue culture seedlings such as temperature and humidity, nutrient solution concentration and pH bad control

Method used

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Embodiment Construction

[0013] The present invention is further described below:

[0014] The present invention includes the following steps:

[0015] Step 1: According to calcium nitrate 0.5 g / L, ammonium nitrate 0.044 g / L, dipotassium hydrogen phosphate 0.14 g / L, potassium sulfate 0.026 g / L, magnesium sulfate 0.13 g / L, ferrous sulfate 0.013 g / L, sulfuric acid Copper 0.002 g / L, manganese sulfate 0.0043 g / L, boric acid 0.0047 g / L, zinc sulfate 0.0014 g / L, sodium molybdate 0.0003 g / L, and Chlorine 0.0063 g / L to configure the nutrient solution. The conductivity of the nutrient solution is 1.0ms / cm, and the pH is 5.6;

[0016] Step 2: Cut off the roots of the tissue culture seedlings, remove the middle and lower leaves, keep only the upper 2-3 leaves, and insert them into the seedling tray along the drainage holes of the seedling tray to ensure that the bottom of the seedlings is in the nutrient solution, and the plastic basket After all the seedling trays inside are full of cuttings, seal the plastic...

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Abstract

The invention discloses a root induction and seedling exercise method of tissue culture seedlings of virus-free potatoes. According to the method, the seedlings cultivated for 5-6 weeks are adopted, and the seedlings are flourishing in root system, stout in stalk, flourishing in stem leaf and high in adaptability after being transplanted, so that a good foundation is laid for the next production of foundation seeds of the virus-free potatoes. The method is easy in operation and high in efficiency, all seedling-raising disks and plastic baskets used in the process can be used repeatedly, cost is greatly lowered, spreading of bacterial diseases can be effectively controlled, and the growth environment of the tissue culture seedlings can be accurately adjusted and controlled.

Description

technical field [0001] The invention relates to a potato cultivation method, in particular to a method for inducing rooting and hardening of potato tissue culture seedlings. Background technique [0002] At present, the potato tissue culture seedlings are generally hardened on the seedbed in the Wimbledon room, and the roots are not removed. The method is to take the grown tissue culture seedlings out of the culture bottle, and some use the form of floating seedlings to cut the tissue culture seedlings. Float on the prepared nutrient solution on the foam board; some are cultivated by cuttings on the substrate, the substrate is one or a mixture of perlite, vermiculite, and humus, and the nutrient solution is sprayed to complete the seedlings in the hardening stage. Can be transplanted and planted. [0003] The shortcoming of the prior art is: 1, the potato tissue culture seedling is still not strong enough after the hardening, especially the root system is not developed; 2, ...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A01G31/00
Inventor包媛媛张新永郑璐
OwnerYUNNAN AGRICULTURAL UNIVERSITY