A method for the detection of microcystin-lr with palladium-gold alloy nano-cage immunochromatographic test strips

A technology of immunochromatographic test paper and microcystin, which is applied in the field of immunoassay chemistry, can solve the problem of unsuitable preservation of gold nanoparticle sol, and achieve the effect of stabilizing the conjugate
CN103995122BActive Publication Date: 2015-08-26YUNNAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
YUNNAN NORMAL UNIV
Publication Date
2015-08-26

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Abstract

The invention relates to a method for detecting microcystin-LR through a palladium-gold alloy nanoscale cage immune chromatography test strip, and belongs to the technical field of immunity chromatography chemistry. The method comprises the following steps: preparing a Pd-Au bimetal nanoscale cage with a hollow structure by taking Pd nano-particles as seed crystals; spraying a monoclonal antibody for labeling the microcystin-LR to a combined pad; spraying BSA (Bovine Serum Albumin) coupling microcystin-LR on a detection line; spraying goat anti-rat IgG on a control line to prepare the immune chromatography test strip for fast detecting the microcystin-LR in a water body; and carrying out quantitative analysis on the microcystin-LR contained in a test sample by a competition immune chromatography and by reading the grey value of gold nanoscale cage-microcystin antibody mixture remained on the detection line. According to the method, because the adhesion of sulfhydryl and amido in a protein molecule of a Pd-Au alloy is higher than the binding force of a single-metal gold nanoscale cage by virtue of the synergistic action of two metal elements in the alloy, a microcystin antibody is easier to label by that Pd-Au alloy nanoscale cage than by the gold nanoscale cage, and the formed Pd-Au alloy nanoscale cage-microcystin antibody mixture is more stable.
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Description

technical field

[0001] The invention belongs to the technical field of immunoanalysis chemistry. Background technique

[0002] For freshwater lakes with serious eutrophication, the temperature rise every summer will cause blue-green algae to bloom and cause serious pollution. In the large family of cyanobacteria, some species can secrete strong toxins [see Shi Hongxing, Qu Jiuhui, Wang Aimin, etc., Preliminary Research on Blue-green Algae and Algal Toxins in Guanting Reservoir, Chemical Journal of Chinese Universities, 2005, 26(9): 1650-1652], and microcystins (microcystins, MCs) are typical representatives. Among them, MCs-LR containing leucine and arginine at positions 2 and 4 is the most likely and most toxic cyclic heptapeptide hepatotoxin known, and is a cancer-promoting factor in freshwater algae [see Yu S.Z. , Chen G., Zhi X.L., et al.Primary liver cancer: natural toxins and prevention in China(J).Toxicol Sci,1998,23(Suppl2):143-148; and see Yu Shunzhang, Zhao Ning, ...

Claims

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