Chitosan-polypeptide complex for health product preparation and preparation method thereof

A polypeptide complex and chitosan technology, applied in the fields of application, food ingredients, food science, etc., can solve problems such as poor solubility, poor absorption, and difficulty in exerting efficacy, and achieve anti-side effects, promote blood vessel formation, and reduce free radicals the effect of

Active Publication Date: 2016-04-06
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its solubility is relatively poor under sexual conditions, and its absorption by the human body is poor, making it difficult to exert its efficacy

Method used

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  • Chitosan-polypeptide complex for health product preparation and preparation method thereof
  • Chitosan-polypeptide complex for health product preparation and preparation method thereof
  • Chitosan-polypeptide complex for health product preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of chlorinated chitosan-glutathione complex with a degree of substitution of 10%

[0034] (1) Weigh 0.5g of chlorinated chitosan, dissolve in 100ml of deionized water, and set aside.

[0035] (2) Dissolve 0.78g MAL-PEG-NHS in 10ml PBS (pH=8.0), add it dropwise to the solution prepared in step (1), and stir at room temperature for 2h.

[0036] (3) The product obtained in step (2) was ultrafiltered (Ultra-15, molecular weight cut-off 10KDa) with an ultrafiltration tube, and the product was dissolved in 100 ml of PBS (pH=7.0).

[0037] (4) Dissolve 0.093 g of glutathione in 10 ml of deionized water, and add it dropwise to the solution obtained in step (3).

[0038] (5) After stirring at room temperature for 24 hours, the mixed liquid was put into a dialysis bag for dialysis for 48 hours. Freeze-drying to obtain chlorinated chitosan-glutathione complex (CSCl-GSH2).

Embodiment 2

[0039] Example 2 Preparation of Chlorinated Chitosan-Glutathione Complex with a Degree of Substitution of 20%

[0040] (1) Weigh 0.5g of chlorinated chitosan, dissolve in 100ml of deionized water, and set aside.

[0041] (2) Dissolve 0.156g MAL-PEG-NHS in 10ml PBS (pH=8.0), add it dropwise to the solution prepared in step (1), and stir at room temperature for 2h.

[0042] (3) The product obtained in step (2) was ultrafiltered (Ultra-15, molecular weight cut-off 10KDa) with an ultrafiltration tube, and the product was dissolved in 100 ml of PBS (pH=7.0).

[0043] (4) Dissolve 0.186 g of glutathione in 10 ml of deionized water, and add it dropwise to the solution obtained in step (3).

[0044] (5) After stirring at room temperature for 24 hours, the mixed liquid was put into a dialysis bag for dialysis for 48 hours. Freeze-drying to obtain chlorinated chitosan-glutathione complex (CSCl-GSH4).

Embodiment 3

[0045] Example 3 Preparation of chlorinated chitosan-glutathione complex with a degree of substitution of 30%

[0046] (1) Weigh 0.5g of chlorinated chitosan, dissolve in 100ml of deionized water, and set aside.

[0047] (2) Dissolve 2.36g MAL-PEG-NHS in 10ml PBS (pH=8.0), add it dropwise to the solution prepared in step (1), and stir at room temperature for 2h.

[0048](3) The product obtained in step (2) was ultrafiltered with an ultrafiltration tube (Ultra-15, molecular weight cut-off 10KDa), and the product was dissolved in 100ml of PBS (pH=7.0).

[0049] (4) Weigh 0.278g of glutathione and dissolve it in 10ml of deionized water, and add it dropwise to the solution obtained in step (3).

[0050] (5) After stirring at room temperature for 24 hours, put the mixed liquid into a dialysis bag and dialyze for 48 hours. Chlorinated chitosan-glutathione complex (CSCl-GSH6) was obtained by freeze-drying.

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Abstract

The invention discloses a chitosan-polypeptide complex for preparing health products and a preparation method for the complex, and belongs to the technical field of health food. The chitosan-polypeptide compound is formed by linking a functional polypeptide such as glutathione or RoY with a chitosan macro-molecule via covalent bonds. Chlorinated chitosan-glutathione has good anti-oxidation characteristic, and chlorinated chitosan-RoY has the effects of promoting formation of blood vessels and improving blood circulation. The two complexes both have the effects of adjusting pH value of human body, changing acidic physique, enhancing endocrine adjustment and activating hormone, have the efficacy of removing heavy metals in bodies, removing pesticide toxin in bodies and removing excess nutrition in bodies, and can be used as a main composition of a health product.

Description

technical field [0001] The invention belongs to the technical field of health food, and in particular relates to a chitosan-polypeptide complex for the preparation of health food and a preparation method thereof. Background technique [0002] Oxidation is the biggest threat to skin aging and organ failure. Sun exposure, stress, and environmental pollution can flood the skin with free radicals, resulting in oxidative phenomena such as dull complexion and dehydration. All are the "culprits" of oxidation in the body. Supplementing antioxidant substances is beneficial to exercise the body to reduce the production of free radicals or accelerate their removal, so as to counteract the side effects of free radicals and delay the aging of organs. Chitosan has a large number of active groups such as amino and hydroxyl on its molecular chain, and has good biocompatibility and degradation performance. It has good antioxidant properties and has been widely accepted as the "sixth elemen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00A23L29/30A23L33/18
CPCA23L29/30A23L33/17A23V2002/00A23V2250/55A23V2250/511A23V2200/30
Inventor 王常勇李俊杰舒瑶郝彤
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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