A kind of SNP molecular marker primer of pear fruit sepal state main effect qtl locus and its application

Abstract

The invention discloses SNP (single-nucleotide polymorphism) molecular marker primers for pear fruit sepal state major QTL (quantitative trait locus) site and application thereof. According to the SNP marker Pybd06_026, the forward primer CALYX-F is disclosed as SEQ ID NO.1, and the reverse primer CALYX-R is disclosed as SEQ ID NO.2. The specific primers can be used for performing favorable typing on the sepal state on the basis of a high-resolution dissolution curve, i.e. detecting the difference between the polymorphism expression sepal states on the QTL site qCalyx. The molecular marker primers for pear fruit sepal state major QTL can be used for molecular marker assisted selective breeding of pear fruit sepal state, and have important theoretical and practical instruction meanings for accelerating the pear variety genetic improvement progress and enhancing the breeding selection efficiency.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and provides a SNP molecular marker primer for the main effect QTL site of pear fruit sepal state and application thereof. Background technique [0002] my country is one of the most important origins of Pyrus L. in the world, with rich genetic diversity. The cultivars of pears are widely distributed, and they are cultivated except in Hainan Province and Taiwan Province. Pear fruit has high nutritional value, sweet and juicy, and is deeply loved by consumers. Sepals refer to the outermost round of the flower, the pieces that make up the calyx, generally leaf-shaped, and have the function of protecting the flower buds during the flowering period. The sepals usually fall off after the flowers open, but there are also some varieties of pear fruit whose sepals still exist until the fruit matures, which is called persistent calyx. According to whether the sepals of pear fruit are persisten...

AI Technical Summary

Problems solved by technology

[0004] At present, the research on QTL mapping of quantitative traits of pear is still in its infancy. Existing research is mainly aimed at some diseases or growth traits, QTL mapping of pear fruit sepal status and detection of polymorphisms at QTL loci expressing differences in sepal status. The development and application of SNP molecular markers has not been reported

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