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A method of assembling a stirred fermenter system into a fluidized bed enzyme reactor

A technology for enzyme reactors and fermenters, applied in the design and transformation of bioreactors, and in the field of bioengineering, can solve problems such as difficult adaptation, large differences in substrate physical and chemical properties, differences in enzyme catalytic kinetic properties and enzyme stability, etc.

Active Publication Date: 2016-01-13
LAIZHOU SENSIR BREWING FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many types of enzymes used in industrial enzyme-catalyzed reactions, and the forms of enzyme catalysts (immobilized enzymes, free enzymes, immobilized cells, free cells, etc.) There are differences, and all these factors require the design of enzyme reactors that meet the requirements of the catalytic process. However, the current commercial enzyme reactors are mainly stirred tank reactors, which are difficult to meet the requirements of the rapidly developing enzyme engineering technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Agitated biological fermenter is assembled as fluidized bed enzyme reactor to produce 6-aminopenicillanic acid

[0016] Connect the water inlet and outlet of the constant temperature water bath circulation device of the stirring fermenter to the inlet and outlet of the jacket of the chromatographic column with a silicone hose, and the effective length and width of the chromatographic column are 40cm and 8cm respectively. Add immobilized penicillin acylase (107U / g) from the upper port of the chromatography column in a static way, and rinse with pH 8.0 buffer to balance. The height of the immobilized enzyme after pre-equilibration is 1% of the actual height of the chromatography column / 4. Insert the pH electrode of the stirred fermenter into the upper port of the chromatographic column, and at the same time connect the external hoses of the enzyme-catalyzed substrate solution feed bottle, acid bottle and alkali bottle to the peristaltic pump of the stirred ...

Embodiment 2

[0017] Example 2: Assembling a stirred biofermentation tank as a fluidized bed enzyme reactor to produce pea protein polypeptides

[0018] Connect the water inlet and outlet of the constant temperature water bath circulation device of the stirring fermenter to the jacket inlet and outlet of the chromatographic column with a silicone hose, and the effective use length and width of the chromatographic column are 85cm and 10cm respectively. Immobilize Bacillus subtilisin (352U / g) and rinse with pH7.5 buffer to equilibrate. The height of the immobilized enzyme after pre-equilibration is 1 / 5 of the actual height of the chromatography column. Insert the pH electrode of the stirred fermenter into the upper port of the chromatographic column, and at the same time connect the external hoses of the enzyme-catalyzed substrate solution feed bottle, acid bottle and alkali bottle to the peristaltic pump of the stirred fermenter, in which the acid-base supplement The hose outlet of the feed ...

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Abstract

The invention provides a method for assembling a stirring biological fermentation tank into a fluidized bed enzyme reactor, belonging to the technical field of bioreactor design and transformation. Connect the water bath system of the stirring biological fermentation tank to the jacketed water inlet and outlet with an interlayer chromatography column, and use the peristaltic pump on the fermentation tank system to quickly press the substrate solution from the lower port into the chromatography column with immobilized enzymes , the immobilized enzyme was suspended and fully contacted with the substrate solution. The invention utilizes a stirring fermenter equipment system to assemble a fluidized bed enzyme reactor, realizes an enzyme-catalyzed reaction with automatic temperature and pH control, saves equipment funds and reduces site occupation.

Description

technical field [0001] The invention belongs to the technical field of bioreactor design and transformation in the field of bioengineering, and relates to a method for quickly assembling a stirring fermenter into a fluidized bed enzyme reactor. Background technique [0002] When enzymes, especially immobilized enzymes, catalyze reactions in vitro, they must be carried out in a certain reaction vessel in order to control various conditions and speeds of enzyme-catalyzed reactions. The enzyme reactor is the core device used to complete the enzymatic reaction. It provides a suitable place and optimal reaction conditions for the enzyme-catalyzed reaction. It is at the center of the enzyme-catalyzed reaction process and is a bridge connecting raw materials and products. [0003] The characteristic of the fluidized bed reactor is that when the substrate solution passes through the immobilized enzyme column bed upwards from the bottom of the reactor at a sufficiently large flow rat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/40C12M1/36C12M1/12C12M1/02
CPCC12M21/18C12M25/20C12M29/00C12M41/22C12M41/26
Inventor 程仕伟孙虎山梁建光李维卫
Owner LAIZHOU SENSIR BREWING FOOD