Method for assembling stirring type fermentation tank system into fluidized bed type enzyme reactor

Abstract

The invention provides a method for assembling a stirring type fermentation tank system into a fluidized bed type enzyme reactor, belonging to the technical field of bioreactor design and transformation. The method comprises the following steps: externally connecting a water bath system of the stirring type fermentation tank with a jacket water inlet and outlet of an interlayer chromatographic column, pressing a substrate solution into the chromatographic column with immobilized enzyme rapidly from a lower hole by using a peristaltic pump on the fermentation tank system, suspending the immobilized enzyme and sufficiently contacting the immobilized enzyme with the substrate solution. The stirring type fermentation tank system is assembled into the fluidized bed type enzyme reactor, an enzyme catalysis reaction in which that the temperature and the pH value are automatically controlled is achieved, the equipment investment is saved, and the field occupation is reduced.

Description

technical field [0001] The invention belongs to the technical field of bioreactor design and transformation in the field of bioengineering, and relates to a method for quickly assembling a stirring fermenter into a fluidized bed enzyme reactor. [0002] Background technique [0003] When enzymes, especially immobilized enzymes, catalyze reactions in vitro, they must be carried out in a certain reaction vessel in order to control various conditions and speeds of enzyme-catalyzed reactions. The enzyme reactor is the core device used to complete the enzymatic reaction. It provides a suitable place and the best reaction conditions for the enzymatic reaction. It is in the center of the enzymatic reaction process and is a bridge connecting raw materials and products. [0004] The characteristic of the fluidized bed reactor is that when the substrate solution passes through the immobilized enzyme column bed upwards from the bottom of the reactor at a sufficiently large flow rate, ...

AI Technical Summary

Problems solved by technology

[0005] There are many types of enzymes used in industrial enzyme-catalyzed reactions, and the forms of enzyme catalysts (immobilized enzymes, free enzymes, immobilized cells, free cells, etc.) There are differences, and all these factors require the design of enzyme reactors that meet the requirements of the catalytic process. However, the current commercial enzyme reactors are mainly stirred tank reactors, which are difficult to meet the requirements of the rapidly developing enzyme engineering technology.