Semi-nested RT-PCR detection method of canine astrovirus

A technology of RT-PCR and detection method, which is applied in the field of half-set RT-PCR detection of canine astrovirus, can solve the problems of specificity and sensitivity limitations, achieve specificity and sensitivity improvement, shorten the detection cycle, Overcoming the effect of long detection cycle
CN104178581AInactive Publication Date: 2014-12-03SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2014-12-03
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention provides a semi-nested RT-PCR detection method of canine astrovirus. The method comprises the following steps: 1, designing a primer with the conservative fragment of canine astrovirus gene as a target; 2, processing a detection sample, and extracting total RNA; 3, reversely transcribing the total RNA into cDNA; and 4, detecting the canine astrovirus in the sample through the semi-nested RT-PCR method. The method has the characteristics of high sensitivity, good specificity, simplicity, fastness and the like, and is suitable for the pathogenic diagnosis and the epidemiological studies of the canine astrovirus.
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Description

[0001] This application is a divisional application of the following applications: filing date: December 23, 2011; application number: 201110440713.6; invention title: "half-set RT-PCR detection method for canine astrovirus". technical field

[0002] The invention relates to biotechnology, in particular to a half-set RT-PCR detection method for canine astrovirus. Background technique

[0003] Astroviruses are non-enveloped, single-stranded positive-sense RNA viruses with a five- or six-pointed star shape and belong to the family Astroviridae. Astrovirus was first discovered in 1975 by Madeley and Cosgrove in the stool of children with gastroenteritis detected by electron microscopy. Canine Astrovirus was first discovered in 1980 by Williams when he observed diarrheal dog feces with an electron microscope. Astroviruses can cause symptoms such as vomiting, diarrhea, and fever in humans, dogs, cats, and water leopards, especially in infants, young animals, the elderly, and tho...

Claims

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