Fusarium oxysporum and application thereof in resisting leaf spot disease of dendrobium officinale

A technology of Fusarium oxysporum and Dendrobium candidum, applied in the field of microorganisms, can solve the problems of disease occurrence, slow growth rate and low average yield of Dendrobium candidum, and achieve the effects of easy preservation, good development and application prospects, and easy industrial production

Active Publication Date: 2014-12-10
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Claims
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AI Technical Summary

Benefits of technology

The technical effects described in this patented text are that fuscicerasia rosenbergii (FJS) can be used for preventing diseases caused by dendrobe bacterial fungi or other harmful microorganisms such as phytophthora root rotor blight. This helps reduce the amount of pesticide needed while still maintaining their beneficial properties like healthy crops. Additionally, it provides promising ways to improve food security through its ability to resist insect damage from pests.

Problems solved by technology

This patented technical problem addressed in this patent relates to improving the efficiency of producing crops without harmful substances such as synthetic or organic pesticide residues used for pest management purposes while reducing environmental concerns associated with these compounds.

Method used

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  • Fusarium oxysporum and application thereof in resisting leaf spot disease of dendrobium officinale
  • Fusarium oxysporum and application thereof in resisting leaf spot disease of dendrobium officinale
  • Fusarium oxysporum and application thereof in resisting leaf spot disease of dendrobium officinale

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Embodiment 1

[0021] Analysis of the effect of Fusarium oxysporum JSNL002 on disease resistance of Dendrobium officinale.

[0022] (1) Using cottonseed shell fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and put it in a high-pressure condition of 121 ° C and 101.3 kPa Sterilize for 20 minutes. After cooling the culture medium, inoculate the mycorrhizal fungus JSNL002 under aseptic conditions, and then carry out artificial culture. The specific culture conditions are 28° C., 12 hours of light + 12 hours of darkness, pH 6-8, and cultured for 25 to 35 days. Process the cultured bacterial agent into 1cm 3 Blocks of large or small sizes are placed on the surface of the cultivation substrate of Dendrobium candidum seedlings. The inoculation amount of solid bacterial agent for each clump of Dendrobium candidum seedlings is 5g. After applying ...

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Abstract

The invention discloses fusarium oxysporum, which is classified and named as fusarium oxysporum, wherein strain No. is JSNL002, and is preserved in China General Microbiological Culture Collection Center on August 15, 2014 by the preservation No. of CGMCC No. 9566. The fusarium oxysporum JSNL002 disclosed by the invention has an obvious effect on such aspects as improving leaf spot disease resistance of dendrobium officinale; the fusarium oxysporum can be cultivated through artificial culture, and is rapid in breeding speed, convenient for large-scale production and good in development and application prospect.

Description

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Claims

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Application Information

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Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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