A kind of Fusarium oxysporum and its application in Dendrobium officinale against leaf spot

A technology for Fusarium oxysporum and Dendrobium officinale, which is applied in the field of microorganisms, can solve the problems of low average yield, slow growth rate of Dendrobium officinale, occurrence of diseases, etc., and achieves the effects of easy preservation, good development and application prospects, and simple culture conditions.

Active Publication Date: 2016-08-17
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium officinale mainly depends on artificial cultivation, but artificial cultivation of Dendrobium officinale has slow growth rate, low average yield, and serious disease occurrence

Method used

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  • A kind of Fusarium oxysporum and its application in Dendrobium officinale against leaf spot
  • A kind of Fusarium oxysporum and its application in Dendrobium officinale against leaf spot
  • A kind of Fusarium oxysporum and its application in Dendrobium officinale against leaf spot

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Embodiment 1

[0021] Analysis of the effect of Fusarium oxysporum JSNL002 on disease resistance of Dendrobium officinale.

[0022] (1) Using cottonseed shell fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and put it in a high-pressure condition of 121 ° C and 101.3 kPa Sterilize for 20 minutes. After cooling the culture medium, inoculate the mycorrhizal fungus JSNL002 under aseptic conditions, and then carry out artificial culture. The specific culture conditions are 28° C., 12 hours of light + 12 hours of darkness, pH 6-8, and cultured for 25 to 35 days. Process the cultured bacterial agent into 1cm 3 Blocks of large or small sizes are placed on the surface of the cultivation substrate of Dendrobium candidum seedlings. The inoculation amount of solid bacterial agent for each clump of Dendrobium candidum seedlings is 5g. After applying ...

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Abstract

The invention discloses a kind of Fusarium oxysporum, which is classified as Fusarium oxysporum (Fusarium oxysporum), and the strain number is JSNL002. CGMCC No.9566, the deposit date is August 15, 2014. The Fusarium oxysporum JSNL002 of the present invention has remarkable effects in improving the growth and resistance to leaf spot of Dendrobium candidum, can be artificially cultivated, has a fast reproduction speed, is convenient for large-scale production, and has good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Fusarium oxysporum and its application in disease resistance of dendrobium officinale. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of the genus Dendrobium orchidaceae. Studies have shown that from seed germination to healthy growth of seedlings, there is no need for the participation of symbiotic fungi. As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium officinale mainly depends on artificial cultivation, but artificial cultivation of Dendrobium officinale has slow growth rate, low average yield and serious disease occurrence. At present, disease control still mainly relies on pesticides. From the perspective of protecting the ecological environment and food safety, Dendrobium candidum, as a precious traditional Chinese medicine, should reduce the applicatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/77
Inventor 徐超陈露杨鹤同赵桂华郑凯席刚俊史俊张红岩何任红
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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