A strain of Fusarium solani and its application in the control of Dendrobium officinale

A technology for Fusarium rot and Dendrobium candidum, which is applied in the field of microorganisms, can solve the problems of easy injury of Dendrobium candidum, great destructiveness, rapid onset, etc., achieves good development and application prospects, improves disease resistance, and has remarkable effects.

Active Publication Date: 2019-09-20
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in bed grooves with too large planting density, thunderstorm weather from high temperature to humidity is more prone to disease, especially under the condition of acidic substrate (PH value 3-5), the disease is the most serious. sex big
At present, the control of Dendrobium officinale is through chemical pesticides, which are easy to damage Dendrobium officinale

Method used

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  • A strain of Fusarium solani and its application in the control of Dendrobium officinale
  • A strain of Fusarium solani and its application in the control of Dendrobium officinale

Examples

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Effect test

Embodiment 1

[0023] A dendrobium candidum resistant strain JSNL007-2 was isolated and screened from the plants in the planting area of ​​Dendrobium candidum in Jurong City, Jiangsu Province. It was cultured on a PDA plate. On the surface of the substrate; the opposite side is white to light yellow, with slight pigmentation, such as figure 1 shown.

[0024] The full sequence of the ITS region of the strain JSNL007-2 was amplified and sequenced, and the full sequence of the rDNA ITS sequence obtained by PCR amplification, as shown in SEQ ID No 1, was found by comparison on the genebank, and the results showed that the strain JSNL007-2 The homology with the Fusarium solani strain (Fusarium solani) is the closest, and the homology with the strains Fusarium solani LYF019 and Fusarium solani CDR3P2F2 both reached 99%. Combined with the morphological and physiological and biochemical characteristics, the strain JSNL007-2 was initially identified as Fusarium solani Fusarium solani (Fusarium solan...

Embodiment 2

[0026] Microbial agent produced by Fusarium solani (Fusarium solani) JSNL007-2:

[0027] (1) Using cottonseed shell fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and put it in a high-pressure condition of 121 ° C and 101.3 kPa Sterilize under low temperature for 20-25 minutes, inoculate the mycorrhizal fungus JSNL007-2 under aseptic conditions after the culture medium is cooled, and then carry out artificial cultivation;

[0028] (2) Specific culture conditions for artificial culture at 25-30°C, 8 hours of light and 16 hours of darkness per day, and a pH of 6.0-6.5 for 5 to 8 days.

[0029] (3) Cut the solid microbial agent cultured in step (2) into 1-1.5cm 3 sized chunks, set aside.

Embodiment 3

[0031] Analysis of the effect of the microbial agent produced by Fusarium solani (Fusarium solani) JSNL007-2 on the disease resistance of Dendrobium officinale.

[0032] (1) artificially cultivate on the PDA medium, obtain the sclerotia of the white silkworm pathogen;

[0033] (2) The solid microbial bacterial agent cultivated in Example 2, and the sclerotium of the white silk disease pathogen cultivated in step (1), are inoculated on the surface of the cultivation substrate of Dendrobium candidum seedlings simultaneously, wherein, the JSNL007 of every clump of Dendrobium candidum seedlings -2 The inoculum amount of the solid bacterial agent is 5-8g, and the number of sclerotias of the pathogen of white silkworm is 10;

[0034] (3) After applying the bacterial agent, manage the Dendrobium officinale plants in step (2) under the same conditions, and maintain the relative water content of the substrate at least 6-10 days above 60%, so as to ensure that the mycelium survives in t...

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Abstract

The invention discloses a strain of Fusarium solani and its application in the prevention and treatment of Dendrobium candidum officinale. The strain JSNL007‑2, identified as Fusarium solani, has been preserved in China's Microbiological Culture Preservation Management Committee General Microbiology Center, the deposit number is CGMCC NO.13687, and the deposit date is March 10, 2017. Fusarium solani (Fusarium solani) JSNL007-2 of the present invention has remarkable effect on disease resistance of Dendrobium officinale, especially for improving the ability of Dendrobium officinale to resist white silk disease, has a certain effect on reducing the use of chemical pesticides, and is a guarantee for edible important means of safety. The microbial bacterial agent produced by the strain JSNL007‑2 can significantly improve the resistance of Dendrobium officinale to bacterial blight, has simple culture conditions, is easy to preserve, and is easy to industrialized production, and has good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Fusarium solani and its application in preventing and treating dendrobium officinale white silk disease. Background technique [0002] Dendrobium (Dendrobium) is a perennial herbaceous plant in the family Orchidaceae, and there are more than 1,500 species in the world. The 2010 edition of "Chinese Pharmacopoeia" divides the recorded Dendrobium into two categories, namely Dendrobium officinale and Dendrobium candidum. The latter Dendrobium officinale (D. officinale Kimuraet Migo) has good nourishing and health benefits in addition to good medicinal effects. Effect. Dendrobium officinale has small leaf area, low photosynthetic intensity, and strict requirements on the growth environment. It often grows very slowly on large tree trunks and between stone walls and crevices. Due to excessive mining and damage to the ecological environment, Dendrobium h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/77A01N63/30
CPCC12N1/20A01N63/30C12R2001/77C12N1/145C12N1/14
Inventor 徐超张红岩杨鹤同席刚俊郑凯史俊
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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