A strain of Fusarium solani and its application in Dendrobium candidum against root rot

A technology of Fusarium solani and Dendrobium candidum, applied in the field of microorganisms, can solve the problems of low average yield and slow growth rate of Dendrobium candidum, achieve the effects of improving root rot resistance, good development and application prospects, and improving disease resistance

Active Publication Date: 2019-09-20
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the market demand of Dendrobium officinale mainly depends on artificial cultivation, but artificial cultivation of Dendrobium officinale has slow growth rate, low average yield and serious disease occurrence

Method used

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  • A strain of Fusarium solani and its application in Dendrobium candidum against root rot
  • A strain of Fusarium solani and its application in Dendrobium candidum against root rot

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A Dendrobium officinale root rot-resistant strain JSNL007-1 was isolated and screened from the plants in the planting area of ​​Dendrobium candidum in Yingtan City, Jiangxi Province. The strain was cultured on a PDA plate, and the hyphae were loose and fluffy; Off-white, milky yellow on the back, such as figure 1 shown.

[0024] The full sequence of the ITS region of the strain JSNL007-1 was amplified and sequenced, and the complete sequence of the rDNA ITS sequence obtained by PCR amplification, as shown in SEQ ID No 1, was found by comparison on the genebank, and the results showed that the strain JSNL007-1 The homology with the Fusarium solani strain (Fusarium solani) is the closest, and the homology with the strains Fusarium solaniQK1409120101 and Fusarium solani CDR3P2F2 both reached 99%. Combined with the morphological and physiological and biochemical characteristics, the strain JSNL007-1 was initially identified as Fusarium solani Bacteria (Fusarium solani), na...

Embodiment 2

[0026] Microbial agents produced by Fusarium solani (Fusarium solani) JSNL007-1:

[0027] (1) Using cottonseed shell fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and put it in a high-pressure condition of 121 ° C and 101.3 kPa Sterilize under high temperature for 20 minutes, inoculate the mycorrhizal fungus JSNL007-1 under aseptic conditions after the culture medium is cooled, and then carry out artificial cultivation;

[0028] (2) The specific culture conditions for artificial culture are 20-30 days at 27-30°C, 8 hours of light and 16 hours of darkness per day, and pH6.0.

[0029] (3) Cut the solid microbial bacterial agent cultivated in step (2) into 1cm 3 sized chunks, set aside.

Embodiment 3

[0031] Analysis of the effect of microbial agent produced by Fusarium solani (Fusarium solani) JSNL007-1 on the disease resistance of Dendrobium officinale root rot.

[0032] (1) The solid microbial bacterial agent cultivated in Example 2 is placed on the surface of the cultivation substrate of Dendrobium officinale seedlings, and the inoculation amount of the solid microbial agent of each clump of Dendrobium candidum seedlings is 5-6g. After applying the bacterial agent, the relative water content of the substrate is maintained Above 60% for at least 10 days to ensure that the hyphae survive in the matrix.

[0033] (2) Cultivate the root rot pathogenic bacteria on the PDA medium by ultraviolet light and dark treatment, make the pathogenic spore suspension, it is diluted to 2 * 10 5 a / mL;

[0034] (3) Manage the Dendrobium officinale plants of step (1) under the same conditions, select the fifth leaf on the upper part of the test plants, artificially create wounds with acupun...

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Abstract

The invention discloses a strain of Fusarium solani and its application in resisting root rot of Dendrobium officinale. The strain JSNL007‑1, identified as Fusarium solani, has been deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC NO.13686, and the preservation date is March 10, 2017. Fusarium solani (Fusarium solani) JSNL007-1 of the present invention has remarkable effect on disease resistance of Dendrobium officinale, especially for improving the ability of Dendrobium officinale to resist root rot, and has a certain effect on reducing the use of chemical pesticides. important means of safety. The microbial bacterial agent produced by the strain JSNL007‑1 can significantly improve the resistance to root rot of Dendrobium candidum, has simple culture conditions, is easy to preserve, and is easy to industrialized production, and has good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Fusarium solani and its application in resisting root rot of dendrobium officinale. Background technique [0002] Dendrobium is the second largest genus of Orchidaceae, a perennial herb with more than 1,500 species worldwide. The 2010 edition of "Chinese Pharmacopoeia" divides the recorded Dendrobium into two categories, namely Dendrobium officinale and Dendrobium officinale, the latter Dendrobium officinale (D. It is an extremely precious Chinese medicinal material, known as "gold in medicine". [0003] Due to excessive mining and damage to the ecological environment, Dendrobium has been listed as one of the endangered and protected medicinal species in China. At present, the market demand of Dendrobium officinale mainly depends on artificial cultivation, but artificial cultivation of Dendrobium officinale has slow growth rate, low average yield ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/77
CPCC12N1/14C12N1/145C12R2001/77
Inventor 徐超杨鹤同汪善锋席刚俊郑凯史俊
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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