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Azo dye orange II degrading bacterium and inocula produced from same

A technology of azo dyes and degrading bacteria, applied in the biological field, can solve problems such as development of relatively mature technology, pollution, and harm to human beings, and achieve the effects of protecting human health, convenient use, and low production costs

Active Publication Date: 2014-12-10
嘉兴华吉环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Azo dye golden orange II products are soluble in water, the pollution of the environment by azo dye golden orange II has seriously endangered the health of human beings, the comprehensive treatment of its environment, the development of degrading bacteria of azo dye golden orange II in the environment There is no relatively mature technology

Method used

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  • Azo dye orange II degrading bacterium and inocula produced from same
  • Azo dye orange II degrading bacterium and inocula produced from same
  • Azo dye orange II degrading bacterium and inocula produced from same

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Embodiment Construction

[0020] The present invention will be further analyzed below in conjunction with specific embodiments and accompanying drawings.

[0021] The azo dye golden orange II degrading bacteria, whose strain is Microbacterium oxydans strain L-11, was preserved in the General Microorganism Center CGMCC of China Committee for the Collection of Microbial Cultures on May 8, 2014, address: Chaoyang District, Beijing No. 3, No. 1 Yard, Beichen West Road, preservation number CGMCC NO.9130.

[0022] 1. Isolation and identification of strains

[0023] Take 10g of contaminated soil, put it in 100ml of sterile water, and shake it for 5min to get the soil bacteria suspension; take 5ml of the soil bacteria suspension, add it to 100ml of inorganic salt medium [NH 4 NO 3 : 407mg / L; KH 2 PO 4 : 98mg / L; K 2 HPO 4 : 33mg / L; NaCl: 30mg / L; MgSO 4 : 200mg / L; pH7.0], add Golden Orange II as a carbon source, place it at 30°C, and culture it on a shaker at 150r / min to obtain a rich solution, which is c...

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Abstract

The invention discloses an azo dye orange II degrading bacterium and an inocula produced from the same. The bacterial strain is bacterial strain L-11 of microbacterium oxydans, is preserved in China General Microbiological Culture Collection Center since 8th, May, 2014, the preservation address is No. 3, No. 1 yard, Beichen West Road, Chaoyang District, Beijing City, and the preservation number is CGMCC NO.9130. The main biological characteristics comprise that the bacterium colony is circle and is not transparent, the bacterium colony is white after being cultured for 1-3 days and is yellow after being continuously cultured, the liquid culture color is yellow, the optimum culture temperature is 28-30 DEG C, and the optimum pH is 7.2. The bacterium belongs to actinomyces. The degrading bacterium L-11 is capable of enabling the azo dye orange II residual quantity to be reduced by 80% or more. The azo dye orange II degrading bacterium inocula has the advantages of low production cost, usage convenience and good removing effect, and is applicable to pollution treatment of water and soil.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to an azo dye golden orange II degrading bacterium and a bacterial agent produced therefrom, which degrades the dye by using microorganisms and is suitable for the treatment of environmental pollution. Background technique [0002] Due to the variety of microorganisms and various metabolic types, they have great advantages over physical and chemical methods in the treatment of dye wastewater, especially for the treatment of azo dyes by microorganisms. Under aerobic conditions, bacteria cannot metabolize azo dyes well; under anaerobic conditions, many microbial species can break electrophilic azo bonds, and azo dyes are degraded into two colorless aromatic dyes by azo reductase amine. These aromatic amines cannot be further degraded under anaerobic conditions, and are toxic to animals and carcinogenic. However, aromatic amines are effective substrates for aerobic bi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
Inventor 刘秀艳焦亮郭军
Owner 嘉兴华吉环保科技有限公司
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