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Mesenchymal stem cell as well as establishing method and application of mesenchymal stem cell

A bone marrow mesenchymal, stem cell technology, applied in the field of genetic engineering, can solve problems such as lack of HIV-1 infection

Active Publication Date: 2014-12-17
GUILIN MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] However, if BMSCs are HIV-1-infected cells, their cell membranes lack the receptor molecules and HIV-1 co-receptor molecules required for HIV-1 infection.

Method used

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  • Mesenchymal stem cell as well as establishing method and application of mesenchymal stem cell
  • Mesenchymal stem cell as well as establishing method and application of mesenchymal stem cell
  • Mesenchymal stem cell as well as establishing method and application of mesenchymal stem cell

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Embodiment Construction

[0034] The principles and features of the present invention will be described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0035] The invention provides a bone marrow mesenchymal stem cell carrying HIV-1 receptors and HIV-1 co-receptors.

[0036] Preferably, the above-mentioned HIV-1 receptor is a CD4 molecule, and the above-mentioned HIV-1 co-receptor is a CCR5 molecule; the CD4 molecule is located in the above-mentioned bone marrow mesenchymal stem cell and its nucleotide sequence is shown in SEQ ID NO. CD4 gene expression, the CCR5 molecule is expressed by the CCR5 gene located in the bone marrow mesenchymal stem cells and the nucleotide sequence shown in SEQ ID NO.2.

[0037] When HIV-1 infects the above-mentioned BMSCs, the envelope protein gp120 on HIV-1 binds to the above-mentioned HIV-1 receptor molecule, so that the envelop...

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Abstract

The invention relates to a mesenchymal stem cell. The cell carries an HIV-1 (Human Immunodeficiency Virus-1) receptor and an HIV-1 coreceptor. When BMSCs (Bone Marrow Mesenchymal Stems) are infected by HIV-1, an envelope protein gp120 on HIV-1 is combined with the HIV-1 receptor to ensure that gp41 on HIV-1 is exposed, the exposed gp41 is combined with the HIV-1 coreceptor to mediate HIV-1 to enter the BMSCs, and the reverse transcription and replication conditions of HIV-1 in the BMSCs are researched, so that the pathogenesis and pathological characteristics of HIV-1 / AIDS (Acquired Immune Deficiency Syndrome) and HIV-1-resistant drugs are researched.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a bone marrow mesenchymal stem cell and its establishment method and application. Background technique [0002] Human immunodeficiency virus (Human Immunodeficiency Virus, hereinafter referred to as HIV) is a lentivirus that infects cells of the human immune system. , and eventually lead to AIDS. There is currently no effective therapy for this deadly infectious disease. [0003] HIV is divided into type 1 and type 2, and type 1 is the main strain prevalent in the world at present, represented by HIV-1. For HIV-1 infection research, there are two kinds of animal models and cell models. Animal models mainly include non-human primate models, chimeric mouse HIV-infected animal models, etc.; cell models mainly include some CD4-positive cells, such as T4 lymphocytes, mononuclear macrophages, and dendritic cells. These cells have CD4 molecules on their surface, which are HIV-1 re...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N15/867
Inventor 曾常春聂广诸葛福艳李丽君
Owner GUILIN MEDICAL UNIVERSITY
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