Anti-leakage electrophoresis titration electrode solution flow exhaust device and using method thereof

Abstract

The invention discloses an exhaust device for anti-leakage electrophoresis titration electrode liquid flow and a use method thereof. The exhaust device is used for measuring total concentration of protein and mainly comprises an anode circulation chamber, a cathode circulation chamber, an electrophoresis tube, a circulation chamber base, three-way valves, infusion pumps, an anode flushing pump, a cathode flushing pump, hoses, an anode liquid storage bottle, a cathode liquid storage bottle, waste liquid recovery bottles and a camera, wherein the anode liquid storage bottle and the cathode liquid storage bottle are communicated with the anode circulation chamber and the cathode circulation chamber through the hoses respectively, and by means of the flow channel design of the anode circulation chamber and the cathode circulation chamber, bubbles in junction positions of the liquid and gel can be removed conveniently and effectively. According to the device and the method, good flowing passage and leakage control of the electrophoresis liquid can be guaranteed, the bubbles between the electrophoresis liquid and the gel can be removed rapidly and reliably, the smooth circuit is guaranteed, and a simple, safe and reliable experimental environment is provided for the electrophoresis titration experiment.

Description

technical field [0001] The invention relates to the technical fields of analytical chemistry technology, biotechnology, translational medicine, etc., in particular to an anti-leakage electrophoretic titration electrode liquid flow exhaust device and a use method thereof. Background technique [0002] Detecting the total protein content in food is of great significance for measuring the nutritional value of food, determining the price of commodities, and evaluating the quality of food materials (Moore, J.C., DeVries, J.W., Lipp, M., Griffiths, J.C., Abernethy, D.R., 2010, Comper. Rev. Food Sci. Food Saf., 9, 330-357.). And protein content analysis has important applications in clinical diagnosis, such as serum and urine protein quantitative analysis (Kangxixiong, "Clinical Electrophoresis", People's Health Publishing House, 2006). [0003] The existing mainstream protein content detection method is mainly the Kjeldahl method, which has become one of the important standard me...

AI Technical Summary

Problems solved by technology

The device mainly uses a four-way glass tube to realize the communication between the cathode and anode electrode liquid and the colloid in the glass tube. The electrophoresis tube and the four-way glass tube in the device are connected by a rubber tube, which is cumbersome in the actual operation process. , and bubbles are likely to exist in the contact part of the colloid in the glass tube and the electrode solution, and it is difficult to remove them

The presence of air bubbles leads to abnormal electric field distribution, resulting in a decrease in the stability and repeatability of electrophoretic titration

The commonly used method needs to pierce the rubber tube with a syringe needle to suck out the air bubbles, which is destructive and cumbersome to operate

In addition, there are many leakage problems of the electrode solution

The first is that the punctured rubber tube causes the electrode solution to leak

The second is the leakage of the electrode solution when the electrophoresis tube is removed from the hose after the electrophoretic titration

Leakage of acidic and alkaline electrode solutions not only poses serious safety hazards to experimental operators, but also leaks acidic and alkaline electrode solutions that will corrode instruments and equipment

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