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A detection model of zebrafish embryo alcoholic liver and its construction method and application

A zebrafish embryo and detection model technology, applied in the field of biomedical research, can solve the problems of lack of curative drugs, complex etiology of alcoholic liver disease, etc., and achieve the effect of simple observation indicators, easy observation, and reduced yolk absorption

Active Publication Date: 2017-01-18
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the complex etiology of alcoholic liver disease, although various types of drugs have been used clinically for treatment, there is still a lack of effective drugs

Method used

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  • A detection model of zebrafish embryo alcoholic liver and its construction method and application
  • A detection model of zebrafish embryo alcoholic liver and its construction method and application
  • A detection model of zebrafish embryo alcoholic liver and its construction method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The construction method of the zebrafish embryonic alcoholic liver high-throughput detection model described in this embodiment specifically includes the following steps:

[0056] (1) The night before embryo collection, 10 pairs of transgenic adult zebrafish whose adult liver specifically expresses green fluorescence were paired in a mating box with a male-to-female ratio of 1:1, isolated with a partition, and placed in a fish room without light. The next morning, when the lights in the zebrafish fish room were on, remove the partition, let the paired zebrafish mate naturally under light-driven conditions, start timing from the spawning, and collect the eggs within 30 minutes to reduce the risk of fish eggs in the female fish. Due to differences in late embryo development caused by their own differences, after the spawning collection is completed, the paired zebrafish are put back into the breeding system in time, and can be used for spawning again after a 2-week repair ...

Embodiment 2

[0066] The construction method of the zebrafish embryo alcoholic liver high-throughput detection model described in this embodiment specifically includes the following steps:

[0067] (1) The night before the embryos were collected, 10 pairs of transgenic adult zebrafish whose liver specifically expressed green fluorescence were paired in a mating box with a male-to-female ratio of 1:1, separated with a partition, and placed in a fish room without light. When the lights in the zebrafish fish room are on the next morning, remove the partition, let the paired zebrafish mate naturally under light-driven conditions, start timing from the spawning, and collect the eggs within 30 minutes to reduce the risk of the fish eggs themselves in the female fish. Due to differences in late embryo development caused by differences, after the spawning collection is completed, the paired zebrafish are put back into the breeding system in time, and can be used for spawning again after a 2-week rep...

Embodiment 3

[0077] The construction method of the zebrafish embryo alcoholic liver high-throughput detection model described in this embodiment specifically includes the following steps:

[0078] (1) The night before embryo collection, 10 pairs of transgenic adult zebrafish whose adult liver specifically expresses green fluorescence were paired in a mating box with a male-to-female ratio of 1:1, isolated with a partition, and placed in a fish room without light. The next morning, when the lights in the zebrafish fish room were on, remove the partition, let the paired zebrafish mate naturally under light-driven conditions, start timing from the spawning, and collect the eggs within 30 minutes to reduce the risk of fish eggs in the female fish. Due to differences in late embryo development caused by their own differences, after the spawning collection is completed, the paired zebrafish are put back into the breeding system in time, and can be used for spawning again after a 2-week repair per...

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Abstract

The invention belongs to the field of biological medicine research, and particularly relates to a zebrafish embryo alcoholic liver detecting model and a construction method and application of the zebrafish embryo alcoholic liver detecting model. The transgenic zebrafish with liver specific expression fluorescent proteins is adopted by the method to construct a model of the alcoholic liver disease; the zebrafish embryo alcoholic liver detecting model is constructed by analyzing the information of swelling liver appearance, reduction of embryo yolk absorption amount, fluorescent reading and the like of the zebrafish. The model has the characteristics of simple observation index, easiness in observation, capability of being qualitative and quantitative and the like, and is especially suitable for being applied to high throughput screening of medicaments for preventing and treating the alcoholic liver.

Description

technical field [0001] The invention belongs to the field of biomedical research, and in particular relates to a high-throughput detection model of zebrafish embryo alcoholic liver and its construction method and application. Background technique [0002] Alcoholic liver disease (ALD) is divided into alcoholic fatty liver, alcoholic hepatitis, alcoholic liver fibrosis and cirrhosis in histopathology, and has become one of the main liver diseases affecting people's health. It is estimated that 90% of long-term excessive drinking can develop fatty liver, 30%-37% can develop alcoholic liver fibrosis and liver cirrhosis, and 5-15% of patients can still develop alcoholic liver fibrosis and liver cirrhosis even if they quit drinking. cirrhosis of the liver. In my country, patients with alcoholic liver disease have been drinking for a long time and have a large daily drinking volume. According to reports, among the 902 patients with alcoholic liver disease investigated, the avera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 陈志亮罗志毅殷婷婷徐毅敏郑玉清
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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