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A Tissue Culture Rapid Propagation Method of Eucalyptus grandis dh32-26

A technology of tissue culture rapid propagation and varieties, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of inability to effectively inherit the excellent characteristics of parents, tissue culture work has not been carried out effectively, and excellent characteristics need to be studied. , to achieve the effects of improving economic and social benefits, being easy to promote and use, and reducing the risk of tissue culture

Active Publication Date: 2016-08-17
广西壮族自治区国有东门林场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned patent uses the stem section of Eucalyptus urophylla aseptic seedlings as explants to induce embryogenic callus, and then obtains regenerated plants through the regeneration of somatic embryogenesis. The operation is relatively complicated and difficult to implement. The stems of the seedlings are explants, and the excellent characteristics of the female parent need to be studied. The commercial seedling cultivation using this technology alone cannot effectively inherit the excellent characteristics of the parents.
[0008] It can be seen that although there are many studies on the tissue culture technology of eucalyptus at present, there is still a lot of work to be explored for the purposeful propagation of the excellent traits of excellent trees, and the tissue culture and rapid propagation methods that can be effectively popularized and applied. Due to the DH32-26 It is a perennial species of Eucalyptus grandis, and there are few tissue-cultured seedlings in the market, and resources are in short supply, but the tissue culture of this species has not been effectively carried out. The tissue culture research on the new species of Eucalyptus grandis DH32-26 is conducted, and the rapid propagation of this species , to meet the market supply, it is of great significance to improve the ecological and economic benefits of this variety

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  • A Tissue Culture Rapid Propagation Method of Eucalyptus grandis dh32-26

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Effect test

Embodiment 1

[0032] The tissue culture rapid propagation method of the Eucalyptus macrocephalus DH32-26 variety, the specific steps are as follows:

[0033] ①Establishment of explants: Select 4-5 years old trees with strong growth, thick trunks, and no pests and diseases, and cut them down from a place 20-25cm above the ground. When sprouting, the bud strips were taken back as explant material. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0034] ② Disinfection of explants: step ① Soak the obtained explants in 75% alcohol for 30 seconds, rinse them with sterile water for 5 times, drain the water, put them in 0.1% mercuric chloride solution for disinfection for 9 minutes, and rins...

Embodiment 2

[0041] The tissue culture rapid propagation method of the Eucalyptus macrocephalus DH32-26 variety, the specific steps are as follows:

[0042] ①Establishment of explants: Select 4-5 years old trees with strong growth, thick trunks, and no pests and diseases, and cut them down from a place 20-25cm above the ground. When sprouting, the bud strips were taken back as explant material. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0043] ② Disinfection of explants: step ① Soak the obtained explants in 75% alcohol for 30 seconds, rinse them with sterile water for 4 times, drain the water, put them in 0.1% mercuric chloride solution for 9 minutes, and rinse them with steri...

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Abstract

The invention discloses a tissue culture and rapid propagation method of Eucalyptus urophylla DH32-26 variety, which is realized through the steps of explant establishment, explant disinfection, primary culture, subculture, rooting culture, seedling hardening and transplanting. , A special medium composition was selected for primary culture, subculture and rooting culture. Reasonable seedling hardening measures made Eucalyptus macrophylla grow fast after transplanting, with a high survival rate, and achieved the purpose of high reproduction coefficient and good reproduction effect. Simultaneously, the production cost of the method of the invention is low, and the obtained tissue culture seedlings have consistent genetic traits, which is an effective way for tissue culture and rapid propagation of eucalyptus, and has a wide application range.

Description

technical field [0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus macrocephalus DH32-26 variety. Background technique [0002] Eucalyptus grandis DH32-26 variety (E .urophylla × E . Grandis ）， It is Guangxi Dongmen Forest Farm, with Indonesian provenance Eucalyptus urophylla U16 (E ucalyptus urophylla ) as the female parent, and the Australian provenance Eucalyptus grandis G46 (E ucalyptus grandis ) is an excellent eucalyptus species obtained through vegetative propagation as a male parent hybrid dominant species. This species has fast growth and high yield, short rotation period (industrial fiber material); Straight, resistant to barrenness, and suitable for planting in large areas. Years of popularization tests have proved that the average tree height of the 5-year-old Eucalyptus grandis DH32-26 variety is 24.72m, the average diameter at breast height is 1...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 苏勇陈德文梁庭辉沈云莫继有庞贞武黄全东吴兵梁秀莉陈允椿韦炳俭付淼吴满芬俸荣娣邓冬丽龚卫新
Owner 广西壮族自治区国有东门林场
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