Determination method for residual pesticides in food of animal origin

Abstract

The invention belongs to the technical field of food detection, and discloses a method for detecting residual pesticides in an animal derived food. The method comprises the following steps: using pesticide standard substances to prepare single standard solutions, preparing a mixed standard solution according to determination needs, analyzing and determining the mixed standard solution in an online gel permeation chromatograph-gas phase mass spectrograph, and drawing a standard curve; taking the animal derived food, homogenating, carrying out mixed dissolution on the obtained substance in acetonitrile and n-hexane, and carrying out ultrasonic extraction; allowing the obtained extract liquid to go through an extraction column, collecting the obtained purified solution and the obtained eluate, concentrating the purified solution and the eluate, and filtering to a sample solution to be detected; and injecting the sample solution to be detected into the online gel permeation chromatograph-gas phase mass spectrograph, selecting a standard working solution according to the content of pesticides in the sample solution to be detected, carrying out isopyknic mixed sample injection determination on the standard working solution and the sample solution to be detected, carrying out qualitative analysis according to retention time contrast, and carrying out quantitative analysis through an external standard technology. The detection method has the advantages of fast separation of interfering substances, reduction of the detection limit, accurate detection result and wide application range.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a method for detecting residual pesticides in animal-derived foods. Background technique [0002] On August 1, 2014, Hong Kong officially implemented the "Regulation Scheme on Pesticide Residues in Food", which is a new measure adopted by Hong Kong to strengthen the management of pesticide residues in food. The regulatory scheme involves a total of 360 pesticides, 7086 maximum residue limits (MRL) / re-residue limits (EMRL), 1735 group maximum residue limits (MRL) / re-residue limits (EMRL), no maximum Residue limit (MRL) / re-residue limit (EMRL) pesticides are all implemented "set value". The limits of 18 common pesticides in food of animal origin are shown in Table 1: [0003] Table 1. Limit requirements for 18 pesticides in Hong Kong’s regulatory scheme [0004] [0005] [0006] The implementation of the "Residual Pesticides in Food Regulatory Pla...

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Problems solved by technology

[0006] The implementation of the "Residual Pesticides in Food Regulatory Plan" will definitely increase the residue testing items of imported and exported food, and at the same time, it will highlight that the testing standards in the Mainland are not perfect, which will greatly increase the workload of government supervision and monitoring departments. This leads to longer inspection and quarantine cycles, slower customs clearance, and increased export costs

The high risk increases the operating cost of the enterprise, and the high cost also loses the price advantage of the original product export, which will eventually lead to the failure of a large amount of mainland food and agricultural products to be supplied to Hong Kong normally, and even affect the sufficient supply of food in the Hong Kong market. to the stability of the whole society

At present, there is no relevant inspection standard for the detection of methaphene residues in animal-derived foods in China. The existing methods for detecting residual pesticides generally use GC, GC-MS, GC-MSMS, ultra-high performance liquid chromatography- Tandem mass spectrometry, HPLC test methods and off-line thin-layer chromatography, etc., have the limitations of slow detection speed, inaccurate detection results, or small adaptability. The method of determination is of great significance to strengthen food safety

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