Treponema pallidum specific antibody quantitative detection kit and preparation method thereof
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A treponema pallidum, quantitative detection technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity, low positive rate of inspection, inability to provide objective indicators of disease treatment effect and prognosis, etc., to achieve accurate results, Simple operation effect
Active Publication Date: 2016-11-16
ZHONGSHAN HOSPITAL XIAMEN UNIV +1
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[0003] Treponema pallidum cannot be cultured in vitro, and the positive rate of direct etiological dark-field microscopy is not high. Serological tests include cardiolipin antibody and specific antibody detection. The false positive rate of cardiolipin antibody is high and the sensitivity is low.
The syphilis-specific antibody detection methods include fluorescent treponema pallidum antibody absorption test (FTA-ABS), Western-blotting (Western-blot), etc., which have high specificity, but cannot perform quantitative detection, and cannot judge the growth and decline of antibodies in patients. Regular, can not provide an objective indicator of the treatment effect and prognosis of the disease
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Embodiment 1
[0033] see figure 1 , said Treponema pallidum-specific antibody quantitative detection kit embodiment is provided with outer packaging box 1, alkaline phosphatase-labeled monoclonal antibody bottle 2, luminescent substrate bottle 3, Treponema pallidum-specific antibody 1000mIU / mL calibrator bottle 4 , 500mIU / mL calibrator bottle 5, 200mIU / mL calibrator bottle 6, 50mIU / mL calibrator bottle 7, 10mIU / mL calibrator bottle 8, 0mIU / mL calibrator bottle 9, washing liquid bottle 10, recombinant antigen coating Microplate 11; alkaline phosphatase-labeled monoclonal antibody bottle 2, luminescent substrate bottle 3, Treponema pallidum-specific antibody 1000mIU / mL calibrator bottle 4, 500mIU / mL calibrator bottle 5, 200mIU / mL calibrator bottle 6 , 50mIU / mL calibrator bottle 7, 10mIU / mL calibrator bottle 8, 0mIU / mL calibrator bottle 9, washing liquid bottle 10, recombinant antigen-coated microwell plate 11 in the outer packaging box 1; alkaline phosphatase label Monoclonal antibody bottle...
Embodiment 2
[0056] The Treponema pallidum-specific antibody quantitative detection kit is used to detect the Treponema pallidum-specific antibody in clinical samples of patients as follows:
[0057] 1. Specimen processing: Serum: 5mL of venous blood, placed in a 37°C water bath for 30 minutes, centrifuged at 3000g for 10 minutes, and the supernatant was used as a test sample for later use.
[0058] 2. Adding samples: add 100 μL of samples and standard products 1-6 to the reaction plate, and use it as a blank control at the same time;
[0059] 3. Add enzyme-labeled monoclonal antibody: 100 μL of alkaline phosphatase-labeled monoclonal antibody, incubate at 37°C for 30 minutes;
[0060] 4. Washing: After reacting at 37°C for 30 minutes, the measured syphilis-specific antibody competes with the alkaline phosphatase-labeled monoclonal antibody and the syphilis-specific recombinant antigen coated on the microwell plate, and the unbound free components are separated by washing ;
[0061] 5. A...
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Abstract
The invention discloses a kit for quantitative detection of specific antibodies of treponema pallidum and a preparation method thereof and relates to the treponema pallidum. The kit is provided with an external packaging box, an alkaline-phosphatase-labeled monoclonal antibody bottle, a luminous substrate bottle, 6 types of calibrator bottles containing the specific antibodies of the treponema pallidum with different concentrations, a washing liquid bottle and a recombinant-antigen-coated microporous plate, wherein the alkaline-phosphatase-labeled monoclonal antibody bottle, the luminous substrate bottle, the 6 types of calibrator bottles containing the specific antibodies of the treponema pallidum with different concentrations, the washing liquid bottle and the recombinant-antigen-coated microporous plate are arranged in the external packaging box. The preparation method comprises the following steps: preparing specific recombinant antigens of the treponema pallidum, the recombinant-antigen-coated microporous plate, natural membrane protein monoclonal antibodies, alkaline phosphatase labels of the monoclonal antibodies, a luminous substrate, a washing liquid and calibrators successively, and finally assembling into the kit for quantitative detection of the specific antibodies of the treponema pallidum. The kit can be used for quantitative detection of the specificity of the syphilis in clinical specimens and can be used as an objective index of the treating effect and the prognosis of the syphilis.
Description
technical field [0001] The invention relates to Treponema pallidum, in particular to a specific antibody quantitative detection kit for Treponema pallidum and a preparation method thereof. Background technique [0002] Syphilis is a sexually transmitted disease caused by Treponema pallidum. The incidence of syphilis has been increasing year by year in China in recent years. The prevention and control of syphilis has been listed as one of the main tasks of public health services in my country. [0003] Treponema pallidum cannot be cultured in vitro, and the positive rate of direct etiological dark-field microscopy is not high. Serological tests include cardiolipin antibody and specific antibody detection. The false positive rate of cardiolipin antibody is high and the sensitivity is low. The syphilis-specific antibody detection methods include fluorescent treponema pallidum antibody absorption test (FTA-ABS), Western-blotting (Western-blot), etc., which have high specificity,...
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