Method for quick pretreatment of raw materials of lentinan and ultrasonic assisted extraction of lentinan
An ultrasonic-assisted extraction and lentinan polysaccharide technology, which is applied in the extraction field, can solve the problems of irreversible damage to the polysaccharide structure, long air-drying treatment time, affecting production efficiency and the like, and achieves the effects of easy operation, low cost and high-efficiency extraction.
Inactive Publication Date: 2015-02-25
SICHUAN AGRI UNIV
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Problems solved by technology
However, the air-drying time is too long, which affects production efficiency; the drying temperature is too high, which will cause irreversible damage to the polysaccharide structure; the cost of freeze-drying is too high, and it is only suitable for high-end product processing
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[0044] 1. Experimental scheme and method
[0045] 1.1 Sample
[0046] Fresh shiitake mushrooms bought in local market
[0047] 1.2 Sample pretreatment
[0048] 1.2.1 Cleaning: Use a special plastic scraper to remove the mud stains on the surface of an appropriate amount of fresh shiitake mushrooms (if washed with water, the surface polysaccharides will be lost).
[0049] 1.2.2 Cut into pieces: Cut the cleaned fresh mushrooms into pieces, and divide each mushroom into four pieces.
[0050] 1.2.3 Microwave treatment: put the shiitake mushrooms in a suitable vessel, and put them in a microwave oven for 5 times at low heat, 2 minutes each time.
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Abstract
The invention relates to a method for quick pretreatment of raw materials of lentinan and ultrasonic assisted extraction of lentinan. The method comprises the following steps: (1) pretreatment, namely cleaning fresh mushrooms, dicing, putting the diced mushrooms into a microwave oven for low-fire batch treatment, next, feeding the sample into a vacuum fryer, drying, and grinding after oil removal; (2) degreasing; (3) water extraction, namely extracting the degreased dried mushroom powder under the conditions that the material-liquid ratio is 1:20, the ultrasonic operating power is 150W, the extraction temperature is 80 DEG C and the extraction time is 2.5 hours; (4) alcohol precipitation; (5) deproteinization; and (6) micromolecule removal, thereby finally obtaining the purified lentinan. According to the method for quick pretreatment of the raw materials of the lentinan and the ultrasonic assisted extraction of the lentinan, the pretreatment operation is simple and easy to industrially popularize, and the pretreated mushrooms have the water activity lower than 0.6 and can be preserved for a long time at a normal temperature; the extraction conditions are mild and do not cause damage to the structure and the configuration of the lentinan, the extraction rate is high and the cost is low.
Description
technical field [0001] The invention relates to an extraction method, in particular to an ultrasonic-assisted extraction of lentinan and a rapid pretreatment method for raw materials thereof. Background technique [0002] Lentinus edodes, the fruiting body of Pleurotus edodes, is the second largest edible fungus in the world and one of the endemic edible fungi in my country. It is known as the "king of mountain delicacies" among the people. It has high-protein, low-fat properties, so it is an ideal daily nutritional dietary supplement. Chinese medical scientists of all dynasties have famous expositions on shiitake mushrooms, such as: "replenishing qi to prevent hunger, treating wind and blood and benefiting stomach and food". With the continuous in-depth research of modern medicine, the medicinal value and active ingredients of shiitake mushrooms are constantly being discovered, among which the anti-tumor, anti-oxidation, anti-diabetic and other medicinal activities of lent...
Claims
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IPC IPC(8): C08B37/00
Inventor 刘韫滔韩国全胡欣洁刘书亮蒲彪
Owner SICHUAN AGRI UNIV
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