Preparation method and application of RNA interfering medicine for treating pulmonary hypertension syndrome and efficacy validation method thereof

A technology for RNA interference and verification methods, which is applied in biochemical equipment and methods, microbial determination/examination, gene therapy, etc., to achieve good therapeutic effects and good pluripotency.

Inactive Publication Date: 2015-03-04
JIANGXI AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, there is no related research on the treatment of broiler AS by blocking the HIF-1α gene.

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  • Preparation method and application of RNA interfering medicine for treating pulmonary hypertension syndrome and efficacy validation method thereof

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Embodiment Construction

[0022] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0023] see figure 1 , a method for preparing an RNA interference drug for treating broiler ascites syndrome, first constructing a lentiviral expression vector-HIF-1α-shRNAi, and then packaging the HIF-1α-shRNAi recombinant plasmid into a medicament, that is, for the treatment of broiler ascites syndrome RNA interference drugs for disease.

[0024] Among them, the steps of constructing the lentiviral expression vector-HIF-1α-shRNAi are: designing and synthesizing primers according to the HIF-1α gene oligonucleotide sequence reported in Genbank, denaturing the positive and antisense strands of the oligonucleotide sequence, Double-stranded HIF-1α-RNAi was formed after renaturation. Using DNA recombination technology, the HIF-1α-RNAi double strand was directional subcloned into the PU6 promoter to obtain the lentiviral expression vector pGCSIL-GFP-HI...

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Abstract

The invention discloses a preparation method and application of an RNA interfering medicine for treating a pulmonary hypertension syndrome and an efficacy validation method thereof. The preparation method comprises the following steps: firstly, establishing a slow virus expression vector, namely HIF-1alpha-shRNAi; secondly, packaging HIF-1alpha-shRNAi recombinant plasmids into a preparation; thirdly, injecting the plasmids into bodies of broilers, silencing an HIF-1alpha gene, inhibiting expressions of a vascular endothelial growth factor (VEGF), endothelin 1 (ET 1) and an insulin-like growth factor II (IGF - II) of a downstream gene and the like, controlling a pulmonary vascular remodeling (PVR) phenomenon, reducing damage of a cardiopulmonary function and ascites formation and finally achieving the clinical treatment to the pulmonary hypertension syndrome. The preparation method has the advantages of specificity, high efficiency and multifunctionality and the like on an aspect of treatment.

Description

technical field [0001] The invention relates to the technical field of gene therapy, in particular to a method for developing an RNA interference drug for treating broiler ascites syndrome. Background technique [0002] Broiler ascites syndrome (AS), also known as broiler pulmonary hypertension syndrome (Pulmonary Hypertension Syndrome, PHS), is caused by various factors causing broiler relative hypoxia or broiler growth too fast, lung volume and body weight increase In this case, because oxygen cannot meet the normal needs of the body, it will show pathological phenomena such as blood viscosity, increased blood volume, hepatomegaly, pulmonary stasis, edema, and pulmonary hypertension. Hypertrophy and dilation of the right ventricle are the main features. It is regarded as a nutritional and metabolic disease, and it is also recognized as one of the diseases that seriously endanger the broiler farming industry. The disease occurs frequently in broiler chickens, and is prone ...

Claims

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Application Information

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IPC IPC(8): A61K48/00C12Q1/68G01N33/68C12Q1/02A61P11/00A61P9/00A61P7/10
Inventor 刘平胡国良郭小权曹华斌张彩英刘佩侯小露黄云飞
Owner JIANGXI AGRICULTURAL UNIVERSITY
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