Chicken sperm diluting liquid and preparation method thereof
A technology of diluent and semen, applied in the field of diluent, can solve the problem of no diluent formula, and achieve the effect of prolonging sperm survival time, reducing stress and improving sperm motility
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[0013] Example 1
[0014] A chicken semen diluent, using sterile distilled water as the solvent, and each 1000ml diluent is composed of the following components: 1.0g magnesium chloride hexahydrate, 1.15g potassium citrate, 9.5g sodium acetate trihydrate, 12.5g fructose, sodium glutamate 15.2g, 3g multivitamins, 0.0024mg of cerium chloride, and the rest as a solvent, the chicken semen diluent has a pH of 6.8 to 7.0 and an osmotic pressure of 375-380mmol / L / kg.
[0015] To prepare the above chicken semen diluent, under aseptic conditions, weigh the required amount of magnesium chloride hexahydrate, potassium citrate, sodium acetate trihydrate, fructose, sodium glutamate, and multivitamins to a small amount of sterilized distilled water. Dissolve the bacteria in a beaker, add cerium chloride, then add sterile distilled water to make the volume constant, shake evenly to obtain the chicken semen dilution, and place it in aliquots and keep it at 3°C for later use.
[0016] The method fo...
Example Embodiment
[0017] Example 2
[0018] A chicken semen diluent, using sterile distilled water as the solvent, and each 1000ml diluent is composed of the following components: 1.0g magnesium chloride hexahydrate, 1.15g potassium citrate, 9.5g sodium acetate trihydrate, 12.5g fructose, sodium glutamate 15.2 g, 3 g of multivitamins, 0.0036 mg of cerium chloride, and the rest as a solvent, the pH of the chicken semen diluent is 6.8-7.0, and the osmotic pressure is 375-380 mmol / L / kg.
[0019] To prepare the above chicken semen diluent, under aseptic conditions, weigh the required amount of magnesium chloride hexahydrate, potassium citrate, sodium acetate trihydrate, fructose, sodium glutamate, and multivitamins to a small amount of sterilized distilled water. Dissolve the bacteria in a beaker, add cerium chloride, then add sterile distilled water to make the volume constant, shake evenly to obtain the chicken semen dilution, and place it in aliquots and keep it at 3°C for later use.
[0020] The me...
Example Embodiment
[0021] Example 3
[0022] A chicken semen diluent, using sterile distilled water as the solvent, and each 1000ml diluent is composed of the following components: 1.0g magnesium chloride hexahydrate, 1.15g potassium citrate, 9.5g sodium acetate trihydrate, 12.5g fructose, sodium glutamate 15.2 g, 3 g of multivitamins, 0.0246 mg of cerium chloride, and the rest as solvents, the chicken semen diluent has a pH of 6.8 to 7.0, and an osmotic pressure of 375-380 mmol / L / kg.
[0023] To prepare the above chicken semen diluent, under aseptic conditions, weigh the required amount of magnesium chloride hexahydrate, potassium citrate, sodium acetate trihydrate, fructose, sodium glutamate, and multivitamins to a small amount of sterilized distilled water. Dissolve the bacteria in a beaker, add cerium chloride, then add sterile distilled water to make the volume constant, shake evenly to obtain the chicken semen dilution, and place it in aliquots and keep it at 3°C for later use.
[0024] The me...
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