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Chicken sperm diluting liquid and preparation method thereof

A technology of diluent and semen, applied in the field of diluent, can solve the problem of no diluent formula, and achieve the effect of prolonging sperm survival time, reducing stress and improving sperm motility

Active Publication Date: 2015-03-11
台山市赛科农业技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the research on diluents of chicken semen is gradually increasing, there is still no ideal diluent formula

Method used

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  • Chicken sperm diluting liquid and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0014] A diluent of chicken semen, using sterilized distilled water as a solvent, and every 1000ml of diluent consists of the following components: 1.0g of magnesium chloride hexahydrate, 1.15g of potassium citrate, 9.5g of sodium acetate trihydrate, 12.5g of fructose, and sodium glutamate 15.2g, 3g of multivitamins, 0.0024mg of cerium chloride, and the rest are solvents, the pH value of the chicken semen dilution is 6.8-7.0, and the osmotic pressure is 375-380mmol / L / kg.

[0015] The method for preparing the above-mentioned diluent of chicken semen is aseptically taking the required amount of magnesium chloride hexahydrate, potassium citrate, sodium acetate trihydrate, fructose, sodium glutamate, and multivitamin until it contains a small amount of sterilized distilled water. Dissolve in a bacterial beaker, add cerium chloride, then add sterilized distilled water to constant volume, shake well to obtain the chicken semen dilution, and divide and store at 3°C ​​for future use. ...

Embodiment 2

[0018] A diluent of chicken semen, using sterilized distilled water as a solvent, and every 1000ml of diluent consists of the following components: 1.0g of magnesium chloride hexahydrate, 1.15g of potassium citrate, 9.5g of sodium acetate trihydrate, 12.5g of fructose, and sodium glutamate 15.2g, 3g of multivitamins, 0.0036mg of cerium chloride, and the rest are solvents, the pH value of the chicken semen dilution is 6.8-7.0, and the osmotic pressure is 375-380mmol / L / kg.

[0019] The method for preparing the above-mentioned diluent of chicken semen is aseptically taking the required amount of magnesium chloride hexahydrate, potassium citrate, sodium acetate trihydrate, fructose, sodium glutamate, and multivitamin until it contains a small amount of sterilized distilled water. Dissolve in a bacterial beaker, add cerium chloride, then add sterilized distilled water to constant volume, shake well to obtain the chicken semen dilution, and divide and store at 3°C ​​for future use. ...

Embodiment 3

[0022] A diluent of chicken semen, using sterilized distilled water as a solvent, and every 1000ml of diluent consists of the following components: 1.0g of magnesium chloride hexahydrate, 1.15g of potassium citrate, 9.5g of sodium acetate trihydrate, 12.5g of fructose, and sodium glutamate 15.2g, 3g of multivitamins, 0.0246mg of cerium chloride, and the rest are solvents, the pH value of the chicken semen dilution is 6.8-7.0, and the osmotic pressure is 375-380mmol / L / kg.

[0023] The method for preparing the above-mentioned diluent of chicken semen is aseptically taking the required amount of magnesium chloride hexahydrate, potassium citrate, sodium acetate trihydrate, fructose, sodium glutamate, and multivitamin until it contains a small amount of sterilized distilled water. Dissolve in a bacterial beaker, add cerium chloride, then add sterilized distilled water to constant volume, shake well to obtain the chicken semen dilution, and divide and store at 3°C ​​for future use. ...

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Abstract

The invention discloses a chicken sperm diluting liquid. The sterile steamed water is taken as the solvent, and each 1000 mL of the diluting liquid comprises the following components by weight: 1.0 gram of magnesium chloride hexahydrate, 1.15 grams of potassium citrate, 9.5 grams of sodium acetate trihydrate, 12.5 grams of fructose, 15.2 grams of sodium glutamate, 3 grams of composite vitamin, 0.0024 to 0.0246 mg of cerium chloride, and the balance being solvent. The pH value of the chicken sperm diluting liquid is 6.8 to 7.0, and the penetration pressure is 375-380 mmol / L / kg. Compared to the conventional chicken sperm diluting liquids, the provided chicken sperm diluting liquid can improve the sperm activity, effectively prolong the sperm survival time, prominently increase the utilizing rate of breeding cocks, and reduce the phenomenon of stress reactions and oviduct inflammation of hens during the artificial insemination process.

Description

technical field [0001] The invention relates to a diluent, in particular to a chicken semen diluent and a preparation method thereof. Background technique [0002] With the popularization and development of cage chickens, the artificial insemination of chickens has been widely promoted. Chicken artificial insemination technology is not difficult, easy to master, simple equipment, less investment. Artificial insemination of chickens can reduce the amount of breeding roosters. During natural mating, the ratio of male to female chickens is about 10-15. When artificial insemination is used, if insemination is done once a week, 1 rooster can be matched with 100 chickens. Even more hens. In production practice, in order to ensure a high fertilization rate of breeding eggs, insemination is generally used every 3-5 days. According to this calculation, the male-to-female ratio of chickens during artificial insemination is mostly about 1:50. Compared with the natural mating method...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张英芹
Owner 台山市赛科农业技术有限公司
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