Yellow green leaf mutant gene YGL6 of rice, protein encoded by yellow green leaf mutant gene YGL6 and application of yellow green leaf mutant gene YGL6

A technology for mutating genes and yellow-green leaves, applied to the protein encoded by the gene and applied in the field of rice yellow-green leaf mutant gene YGL6, which can solve problems such as difficulties, poor traits, and reduced agronomic traits

Inactive Publication Date: 2015-03-11
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since most of the marker traits at the seedling stage are not good in themselves, they often lead to a significant decrease in other important agronomic traits. When using them, a cross-breeding process is required to overcome the genetic burden of bad traits and achieve the aggregation of excellent traits. This process is often It is very difficult and can only be achieved through a large number of long-term transfers

Method used

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  • Yellow green leaf mutant gene YGL6 of rice, protein encoded by yellow green leaf mutant gene YGL6 and application of yellow green leaf mutant gene YGL6
  • Yellow green leaf mutant gene YGL6 of rice, protein encoded by yellow green leaf mutant gene YGL6 and application of yellow green leaf mutant gene YGL6
  • Yellow green leaf mutant gene YGL6 of rice, protein encoded by yellow green leaf mutant gene YGL6 and application of yellow green leaf mutant gene YGL6

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1, Obtaining and Morphological Observation of Rice Yellow-green Leaf Mutant ygl6

[0015] A genetically stable rice yellow-green leaf mutant named ygl6 was obtained by using ethyl methanesulfonate (EMS) to mutate self-fertilizing Jinghui No. 10. The yellow-green leaf mutant ygl6 of rice has yellow-green leaves at the seedling stage, and turns into light green at the late tillering stage to the mature stage ( figure 1 ). After several generations of observation, this trait showed stable inheritance, and there was no significant difference in other main agronomic traits such as panicle length and grain number per panicle, and plant height, effective panicle and thousand-grain weight decreased significantly.

Embodiment 2

[0016] Embodiment 2, mutation ygl6 gene genetic analysis and localization

[0017] The ygl6 mutant was used as the male parent and the indica rice variety Xinong 1A (Xinong1A) was used as the female parent to obtain F1 plants with normal green leaves, and then 8149 F1 plants were obtained by selfing. 2 In the generation population, two phenotypes of mutant leaves and normal leaves were separated according to the traits of yellow-green leaves, and 1997 mutant plants were isolated, and the rest were normal plants. It indicated that the mutant trait was controlled by a pair of recessive single genes.

[0018] Preliminary positioning: 480 pairs of SSR primers evenly distributed on 12 rice chromosomes were selected to detect polymorphisms between parents ygl6 and Xinong 1A, and 98 pairs of SSR primers showed polymorphisms. These 98 pairs of primers were used for gene linkage analysis in the normal and mutant gene pools, and the SSR markers linked to the gene Zebra-15 were screened...

Embodiment 3

[0023] Embodiment 3, clone Os12g23180 gene

[0024] According to the rice Nipponbare gene Os12g23180 sequence registered in GenBank, use Vector NTI software to design mRNA-specific primers for amplifying the YGL6 mutant and wild-type Jinhui No. 10 Os12g23180 sequence: upstream primer YGL6F: 5'-atgtcgtcgccgaccgccg-3' (SEQ ID No .12); downstream primer YGL6R: 5'-atgtcgtcgccgaccgccg-3' (SEQ ID No.13).

[0025] Take 2 g of young leaves of wild-type Jinhui 10 and mutant ygl6 cultured in light for two weeks, put them into liquid nitrogen quickly and grind them into powder, and extract total RNA according to the instructions of the Trizol kit. The results of electrophoresis of total RNA of wild-type Jinhui 10 and mutant ygl6 showed that the main band was clear and complete, and the band brightness ratio of 28S and 18S was about 2:1, indicating that the concentration and purity of RNA met the experimental requirements and could be used for synthesis double-stranded cDNA. Then, using t...

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Abstract

The invention discloses a yellow green leaf mutant gene YGL6 of rice, a protein encoded by the yellow green leaf mutant gene YGL6 and application of the yellow green leaf mutant gene YGL6. The nucleotide sequence of the yellow green leaf mutant gene YGL6 is represented by SEQ ID No.14, and the amino acid sequence of the yellow green leaf mutant gene YGL6 is represented by SEQ ID No.15; compared with a wild type gene, the yellow green leaf mutant gene YGL6 has the advantages that the 11th basic group on the fourth exon is converted from T into A, and the coded amino acid at the fourth site is varied from leucine into a TAG terminator; a rice YGL6 mutant with the mutant gene is yellow green in leaves in the seedling stage and turns into light green from the later tillering stage to the maturation stage; by hybridization, the property is a hidden property, so that new species can be selected and the purity of seeds can be authenticated by the property, and great significance in genetic breeding of rice is achieved.

Description

technical field [0001] The invention belongs to the field of genetics, and specifically relates to rice yellow-green leaf mutation gene YGL6, and also relates to the protein encoded by the gene and its application. Background technique [0002] Rice (Orvza sativa L.) is the most important food crop in the world. In particular, the cultivation of hybrid rice has significantly increased the yield of rice, from 200-300 jin per mu in the past to 800 jin per mu now. Together with the second green revolution, it has almost solved the global food crisis. Great contribution to world food security. However, seed purity is an important factor restricting hybrid rice from playing a greater role. In recent years, the losses caused to farmers due to impure hybrid rice seeds have become more and more serious. This issue has attracted the attention of the government and experts. Seed production of two-line hybrid rice has suffered major losses in recent years. In 1999, due to low temp...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N15/53C12N9/04C12N15/82A01H5/00
Inventor施军琼赵芳明何光华桑贤春凌英华王楠杨正林
OwnerSOUTHWEST UNIVERSITY