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Method to regenerate bone tissue in experiments
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A technology of bone tissue and composition, which is applied in the field of treatment of different bone injuries, and can solve problems such as the regeneration time of long bone tissue
Active Publication Date: 2017-08-01
FELIX LTD
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A disadvantage of the described method as a prototype is also the relatively long bone tissue regeneration time
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Embodiment 1
[0008] Embodiment 1 (preparation of the solution claimed)
[0009] 1.80-2.06g / L 1-hydroxyethylene diphosphonic acid, 1.44-2.22g / L anhydrous calcium chloride, 0.30-0.40g / L gadolinium (III) nitrate hexahydrate, 0.038-0.076g / L of dysprosium(III) chloride hexahydrate and 950ml of distilled water were charged into a 1000ml volumetric flask. The pH of the resulting mixture is adjusted to 7.3-7.8 with any concentrated base solution (eg 10% aqueous sodium hydroxide solution). The resulting solution was adjusted to 1000ml with distilled water, heated to 30°C-100°C, maintained at this temperature for 1-48 hours, and then cooled to room temperature (20-25°C). The resulting solution is then used in the claimed method.
Embodiment 2
[0011] An aqueous solution of рН7.3 with the following composition (g / L) was prepared:
[0012] 1-Hydroxyethylene diphosphonic acid 1.80
[0013] Anhydrous calcium chloride 1.44
[0014] Gadolinium(III) nitrate hexahydrate 0.30
[0015] Dysprosium(III) chloride hexahydrate 0.038
[0016] Allow to reach 30°C, maintain at that temperature for 48 hours, then cool to room temperature. Under anesthesia, 2 ml of the prepared solution was then injected over a period of 2 min into the injured bone of a Nordic cat with a hindlimb fracture induced 1 hour before the experiment. After the injection, the broken bone site is immobilized in a cast or wrapped in a plastic bandage, and the injured leg part is left that way until movement is restored. The regeneration of injured bone tissue was observed with X-rays, using X-ray transparent synthetic Lohmann & Rauscher Cellacast Xtra bandages or special "windows" cut out around the injury site. Based on the behavior of the injured animals, ...
Embodiment 3
[0018] The general procedure of Example 2 was followed, but for injection, 2 ml of an aqueous solution of рН 7.5 with the following composition, g / L, was used:
[0019] 1-Hydroxyethylene diphosphonic acid 1.92
[0020] Anhydrous calcium chloride 1.88
[0021] Gadolinium(III) nitrate hexahydrate 0.35
[0022] Dysprosium(III) chloride hexahydrate 0.055
[0023] It was then heated to 60°C and maintained at this temperature for 6 hours. The bone tissue regeneration times for this case study are shown in Table 1.
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Abstract
The present invention relates to the treatment of various bone injuries, especially fractures and fissure fractures, and can be used in medical and veterinary therapy and surgery. In order to reduce the time required for bone tissue to regenerate at the site of injury or defect and to reduce the time to restore the normal physiological function of the injured bone, a method of regenerating bone tissue is used, which involves immobilizing bone fragments with plaster or a bandage made of a polymeric material, and applying An aqueous solution containing 1.80‑2.06 g / l 1-hydroxyethylidene diphosphonic acid, 1.44‑2.22 g / l anhydrous calcium chloride, 0.30‑0.40 g / l gadolinium nitrate hexahydrate is introduced into the fracture area (III) and 0.038-0.076 g / l dysprosium(III) chloride hexahydrate, and pH 7.3-7.8, wherein said solution is heated to 30°C-100°C before introduction into the fracture area, at said temperature for 1‑48 hours, then cooled to room temperature. As a result, the time required for bone tissue to regenerate at the site of injury was significantly reduced (20‑25%), irrespective of the type of animal in which the injury was present. The above solution for injection has very low toxicity, has good storage properties, and can be stored for a long time without loss of activity.
Description
technical field [0001] The present invention relates to the treatment of various bone injuries. In particular, the invention relates to fractures and fractures, and can be used in medical and veterinary treatment and surgery. Background technique [0002] A method of regenerating bone tissue is known in the art, in which the injured bone fragment is fixed with a plaster, and then an aqueous solution containing 1-hydroxyethylidene diphosphonic acid, calcium chloride and gadolinium (III) nitrate is introduced at room temperature Fracture area [1]. A disadvantage of the described method is the relatively long bone tissue regeneration time. [0003] The method comprising the cumulative features most closely related to the purpose of the present invention and the technical effect achieved is the method of regenerating bone tissue in experiments, which consists of immobilizing the injured bone fragments with plaster or plastic bandages, and subsequently at room temperature will ...
Claims
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Application Information
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