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Method for regenerating bone tissue under experimental conditions

A bone tissue and experimental technology, applied in the direction of tissue regeneration, bone disease, drug combination, etc., can solve the problem of long bone tissue regeneration time and so on

Active Publication Date: 2015-03-11
FELIX LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A disadvantage of the described method as a prototype is also the relatively long bone tissue regeneration time

Method used

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  • Method for regenerating bone tissue under experimental conditions
  • Method for regenerating bone tissue under experimental conditions
  • Method for regenerating bone tissue under experimental conditions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1 (preparation of the solution claimed)

[0009] 1.80-2.06g / L 1-hydroxyethylene diphosphonic acid, 1.44-2.22g / L anhydrous calcium chloride, 0.30-0.40g / L gadolinium (III) nitrate hexahydrate, 0.038-0.076g / L of dysprosium(III) chloride hexahydrate and 950ml of distilled water were charged into a 1000ml volumetric flask. The pH of the resulting mixture is adjusted to 7.3-7.8 with any concentrated base solution (eg 10% aqueous sodium hydroxide solution). The resulting solution was adjusted to 1000ml with distilled water, heated to 30°C-100°C, maintained at this temperature for 1-48 hours, and then cooled to room temperature (20-25°C). The resulting solution is then used in the claimed method.

Embodiment 2

[0011] An aqueous solution of рН7.3 with the following composition (g / L) was prepared:

[0012] 1-Hydroxyethylene diphosphonic acid 1.80

[0013] Anhydrous calcium chloride 1.44

[0014] Gadolinium(III) nitrate hexahydrate 0.30

[0015] Dysprosium(III) chloride hexahydrate 0.038

[0016] Allow to reach 30°C, maintain at that temperature for 48 hours, then cool to room temperature. Under anesthesia, 2 ml of the prepared solution was then injected over a period of 2 min into the injured bone of a Nordic cat with a hindlimb fracture induced 1 hour before the experiment. After the injection, the broken bone site is immobilized in a cast or wrapped in a plastic bandage, and the injured leg part is left that way until movement is restored. The regeneration of injured bone tissue was observed with X-rays, using X-ray transparent synthetic Lohmann & Rauscher Cellacast Xtra bandages or special "windows" cut out around the injury site. Based on the behavior of the injured animals, ...

Embodiment 3

[0018] The general procedure of Example 2 was followed, but for injection, 2 ml of an aqueous solution of рН 7.5 with the following composition, g / L, was used:

[0019] 1-Hydroxyethylene diphosphonic acid 1.92

[0020] Anhydrous calcium chloride 1.88

[0021] Gadolinium(III) nitrate hexahydrate 0.35

[0022] Dysprosium(III) chloride hexahydrate 0.055

[0023] It was then heated to 60°C and maintained at this temperature for 6 hours. The bone tissue regeneration times for this case study are shown in Table 1.

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PUM

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Abstract

The invention relates to the treatment of different bone injuries, particularly fractures and fissure fractures, and can be used in medical and veterinary therapy and surgery. In order to reduce the time taken for bone tissue to regenerate at the site of damage or a defect and to reduce the time taken for the normal physiological functioning of an injured bone to be restored, a method is used for regenerating bone tissue by fixing fragments of the damaged bone with a plaster cast or a bandage made of a polymer material and introducing into the fracture zone an aqueous solution containing 1-hydroxyethylidene diphosphonic acid in an amount of (1.80-2.06) g / l, anhydrous calcium chloride in an amount of (1.44-2.22) g / l, gadolinium (III) nitrate hexahydrate in an amount of (0.30-0.40) g / l and dysprosium (III) chloride hexahydrate in an amount of (0.038-0.076) g / l, with a pH of (7.3-7.8), wherein, prior to being introduced into the fracture zone, the above solution is brought to a temperature of (30-100)°C, is held at this temperature for (1-48) hours and is then cooled to room temperature. As a result, there is a significant (20-25%) reduction in the time taken for bone tissue to regenerate at the injury site, irrespective of the type of animal which has sustained the injury. The above-mentioned solution for injection has low toxicity and good storage properties and can be kept for an extended period of time without loss of activity.

Description

technical field [0001] The present invention relates to the treatment of various bone injuries. In particular, the invention relates to fractures and fractures, and can be used in medical and veterinary treatment and surgery. Background technique [0002] A method of regenerating bone tissue is known in the art, in which the injured bone fragment is fixed with a plaster, and then an aqueous solution containing 1-hydroxyethylidene diphosphonic acid, calcium chloride and gadolinium (III) nitrate is introduced at room temperature Fracture area [1]. A disadvantage of the described method is the relatively long bone tissue regeneration time. [0003] The method comprising the cumulative features most closely related to the purpose of the present invention and the technical effect achieved is the method of regenerating bone tissue in experiments, which consists of immobilizing the injured bone fragments with plaster or plastic bandages, and subsequently at room temperature will ...

Claims

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Application Information

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IPC IPC(8): A61K31/185A61K33/00A61P19/08A61K33/24
CPCA61K31/663A61K33/14A61K33/24A61L24/0015A61L24/02A61L2430/02A61K9/0019A61K47/02A61K9/08A61P19/00A61P19/08A61K2300/00
Inventor F·V·杰维亚托夫
Owner FELIX LTD