Extraction condition optimization method and extraction method of tripterygium multi-index active ingredients

A technology of active ingredients and extraction conditions, which is applied in the field of extraction conditions optimization and extraction of multi-index active ingredients of Tripterygium To achieve the effect of simple extraction, fewer experiments, saving resources and energy consumption

Inactive Publication Date: 2015-03-18
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

However, in the existing Tripterygium wilfordii extraction process, generally only a single index component is selected, and the overall composition of the extract cannot be measured; there are also some reports on the extraction of a class of components such as total lactones and total alkaloids, but the extraction target lacks pertinence, There are many impurities in the extracted medicinal liquid; generally, experimental optimization design is rarely used, so it is impossible to determine whether the extraction process is optimal and scientific and reasonable

Method used

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  • Extraction condition optimization method and extraction method of tripterygium multi-index active ingredients
  • Extraction condition optimization method and extraction method of tripterygium multi-index active ingredients
  • Extraction condition optimization method and extraction method of tripterygium multi-index active ingredients

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Embodiment 1

[0045] A method for optimizing extraction conditions of tripterygium wilfordii multi-index active ingredients

[0046] 1. Extraction experiment

[0047] Weigh the root powder of tripterygium wilfordii with a balance, extract with a mixed solution of one or more components in methanol, ethyl acetate, and acetonitrile, and use a liquid-to-solid ratio of 1:1 to 80:1 between the extraction solution and the powder, Ultrasonic extraction at 50-400W power for 5-60 minutes; solid-liquid separation to obtain an extract containing multiple-index active ingredients of Tripterygium wilfordii. After the extract was filtered through the membrane, it was used for liquid chromatography tandem mass spectrometry detection (R.J.Zhuo, H.B.Xiao et al. Rapid simultaneous quantitative determination of terpenoids in Tripterygium wilfordii Hook F by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry . Analytical Methods, 2013, 5, 2046–2052). Utilizin...

Embodiment 2

[0078] Take the powder of Tripterygium wilfordii medicinal root, add ethyl acetate methanol solution with ethyl acetate volume concentration of 75% according to the liquid-to-material ratio of 32, ultrasonically extract for 30 minutes under 250W ultrasonic power, and separate solid and liquid to obtain Tripterygium wilfordii multi-index Extract of the active ingredient. After the extract was filtered through the membrane, it was used for detection by liquid chromatography tandem mass spectrometry. The calculated OD value is 6.509, and the extraction rate reaches 97.31%.

Embodiment 3

[0080] Take the powder of Tripterygium wilfordii medicinal root, add ethyl acetate methanol solution with a volume concentration of 75% ethyl acetate according to the liquid-to-material ratio of 40, ultrasonically extract for 30 minutes under 250W ultrasonic power, and separate solid and liquid to obtain Tripterygium wilfordii multi-index Extract of the active ingredient. After the extract was filtered through the membrane, it was used for detection by liquid chromatography tandem mass spectrometry. The calculated OD value is 6.513, and the extraction rate reaches 97.37%.

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Abstract

The invention relates to an extraction condition optimization method and an extraction method of tripterygium multi-index active ingredients. The extraction condition optimization method is characterized in that against optimal extraction of the tripterygium multi-index active ingredients, an ultrasonic extraction process of the tripterygium active ingredients in the prior art is integrated, through single-factor experimental investigation, three factors, namely liquid-to-solid ratio, solvent concentration and ultrasonic power, which have relatively great effects on extraction overall desirability value (OD value) of the tripterygium multi-index active ingredients, are selected, design expert software is utilized, and a star point design-response surface optimization method is applied for optimization, so that optimal extraction process parameters are obtained. The methods provided by the invention are simple, the experimental precision is high, the optimal values are calculated by model fitting, resources and energy consumption are saved, and a guide can be provided for industrialized extraction and production.

Description

technical field [0001] The invention relates to a method for optimizing the extraction conditions of the multi-index active components of Tripterygium wilfordii and an extraction method for the active components of Tripterygium wilfordii. Background technique [0002] Tripterygium wilfordii has a long history of clinical use in traditional Chinese medicine and is mainly used to treat various immune and inflammatory diseases, including rheumatoid arthritis and systemic lupus erythematosus. The active ingredients of Tripterygium wilfordii have good anti-tumor, immunosuppressive, anti-inflammatory, anti-fertility, insecticidal and other activities. Triptolide (i.e. triptolide) is the most researched and active ingredient in Triptolide, which has good anti-tumor, immunosuppressive, anti-inflammatory, anti-fertility, insecticidal and other activities. Triptolide ketone and triptolide are similar in structure and activity. Tripterygium wilfordii has strong anti-tumor and good im...

Claims

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Application Information

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IPC IPC(8): C07J75/00C07J73/00C07J63/00
CPCC07J63/008C07J73/003C07J75/00Y02P20/10
Inventor 肖红斌卓荣杰张晓哲王莉高明哲
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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