37results about How to "Best extraction process" patented technology

The method for extracting dioscin and diosgenin includes the following steps: pulverizing rhizome of Chinese yam plant by adding water, fermentation by using lipolytic enzyme, separating cellulose and lignin, recovering supernatant, suspension and starch, mixing recovered liquors and filtering residue, boiling and filtering, repeating for several times, filtering residue for stand-by, combining filtrates and concentrating, using ethyl alcohol to make reflux extraction, cooling and filtering, decompressing and concentrating filtrate, adsorbing, evaporating, ethyl alcohol extracting, adding ethyl ether, cooling to room temp. and separating out tetrasaccharide saponin, recovering ethyl alcohol and ethyl ether from supernatant, precipitating out saponin, drying, adding water in starch and filter residue, regulating pH, adding alpha-amylase.

The invention relates to the technical field of extraction of Chinese traditional medicines and particularly relates to a wild chrysanthemum extraction process and a measuring method for total flavonoids in wild chrysanthemums. The wild chrysanthemum extraction process sequentially comprises the following steps of: ethanol extraction, first concentration and filtration, resin adsorption, second concentration, spray drying and the like to obtain a wild chrysanthemum extract. The wild chrysanthemum extraction process and the measuring method for the total flavonoids in the wild chrysanthemums, provided by the invention, have the advantages that the ethanol heating reflux extraction is carried out on the wild chrysanthemums by using an orthogonal experiment method, the wild chrysanthemum extract is not reacted with chrysanthemum components, the total flavonoid component of the wild chrysanthemums can be dissolved to the maximum extent, and therefore, the optimal extraction process is obtained. The extraction ratio of the total flavonoids in the wild chrysanthemums is up to 6.23%, and the content of the total flavonoids is up to 9.5%. The wild chrysanthemums are low in raw material cost and suitable for large-scale industrial production, and therefore, the extraction development for the flavonoids in the chrysanthemums has higher practical significance. Macroporous resin has the characteristics of high adsorbing capacity and high desorption ratio is adopted.

The invention discloses a method for optimized extraction of total flavonoids of jasmine employing a response surface method. The optimal extraction technology is determined by design expert software according to a Box-Behnken design as follows: the pH value of a solvent is 5.5-6; the liquid-to-solid ratio is (15-20) to 1; the raw materials are dipped for 10-20 minutes; the extraction temperature is 85-92 DEG C; the ultrasonic extraction time is 20-30 minutes; and the natural chromatography time is 0.5-1 hour. The solvent dimethyl carbonate is an environment-friendly solvent integrating cleanness and safety. Diffusion and release of a jasmine active component can be accelerated by cavatition and secondary effect of an ultrasonic wave; the jasmine active component can be fully mixed with a solvent; complete extraction is facilitated; and the yield of the product reaches 98.6%. The method is short in technological process, easy to operate, short in extraction time, low in solvent loss, energy-saving, and environmentally friendly; the product is high in yield and low in cost; the dangerousness in production is greatly reduced; and the method has relatively good economic benefits, social benefits and ecological benefits.

The invention relates to application of a salvianolic acid A composition in preparing medicines for protecting ischemic brain tissue damage. The composition is prepared from 94-97% of salvianolic acid A, 0.1-1.5% of alkannic acid, 0.1-1.5% of rosmarinic acid, 0.1-1.5% of salvianolic acid B and 0.1-2.0% of salvianolic acid C.

The invention relates to a method for ultrasonically extracting total flavones from tussilago farfara. The method comprises the following steps: (1) processing tussilago farfara, namely drying tussilago farfara in a constant-temperature drying box with temperature of 90 DEG C for 12 hours, grinding and sieving a 20-mesh sieve; (2) extracting, namely mixing the tussilago farfara processed in the step (1) with an ethanol solution according to a solution-material ratio (mL / g) of (20:1) to (30:1), and ultrasonically extracting under the power being 350W-500W for 30-45 minutes to obtain a total flavones extracting solution, wherein the volume fraction of ethanol in the ethanol solution is 30%-70%. The ultrasonic power, the ultrasonic extraction time and the material-solution ratio are optimized through a response surface method to obtain an optimal extraction process for ultrasonically extracting the total flavones from the tussilago farfara; the actually measured extraction rate in the process is 6.57%; the extraction condition is accurate and reliable and the extraction rate is relatively high; the method has the advantages of convenience, simplicity, quickness, safety and the like, provides corresponding reference data for extraction, development and utilization of effective ingredients of the tussilago farfara, and has an actual useful value.

The invention relates to optimization of a methyl nutritional type bacillus spore-production fermentation medium. The fermentation medium consists of the following components: 0.5-0.6% of corn meal, 0.08-0.2% of corn starch, 0.9-1.2% of bran, 0.18-0.54% of glucose, 0.05-0.15% of soybean cake powder, 0.16-0.2% of soybean peptone, 0.005-0.006% of KH2PO4, 0.006-0.008% of (NH4)2SO4, 0.004-0.005% of MnSO4, 0.0005-0.002% of CaCO3, 0.005-0.006% of MgSO4.7H2O, and the balance of water. The culture medium provided by the invention not only can meet the requirement of a shake flask fermentation experiment but also can successfully amplify the result of shake flask fermentation to a fermentation tank. Compared with the culture medium before optimization, the spore conversion rate and the spore yield of the formula of the culture medium are improved by 1-10 times, and the formula of the culture medium adopts low-price agriculture byproducts, so that the production cost is greatly reduced, and a foundation is laid for large-scale industrial production of wettable powder.

The invention discloses a method, using a response surface methodology, for optimizing ultrasonic extraction technology of free anthraquinone from cassia seeds. According to the method, cassia seeds are used as a raw material and ultrasonic technology is used to assist extraction of free anthraquinone. The volume fraction of ethanol, the ultrasonic extracting temperature and the ultrasonic extracting time are optimized by using the response surface methodology to obtain an optimum extraction technology for free anthraquinone. The extraction yield of free anthraquinone by using the technology is 28.32%. The extraction conditions are accurate and reliable, the extraction yield is high, and the extraction conditions have use value.

The invention provides a response surface methodology optimized ultrasonic-assisted extraction process for solidago decurrens polysaccharide. The process comprises the following steps: 1, carrying out heating reflux on solidago decurrens powder with 80% ethanol twice, degreasing, decolorizing, removing oligosaccharide and small molecular impurities, and finally volatilizing a solvent to obtain a treated sample; and on the basis of a single-factor test, investigating the influence of independent variables on the extraction yield by utilizing Box-Behnken Designn (BBD) center combination design and utilizing the PPs extraction rate Y (%) as a response value. According to the invention, solidago decurrens is used as a raw material, and the polysaccharide of the solidago decurrens is extracted by adopting an ultrasonic extraction method; and on the basis of a single-factor experiment, the ultrasonic extraction condition of the solidago decurrens polysaccharide is optimized by adopting a response surface method so as to determine the optimal extraction process condition of the solidago decurrens polysaccharide.

The present invention is one kind of medicine composition for treating female climacteric syndrome, endocrine and cardiac and cerebral vascular diseases and its preparation process. The medicine composition is prepared with nine kinds of Chinese medicinal materials, including conic gymnadenia tuber, silverweed cinquefoil root, mango stone, wolfberry fruit, fragrant rhododendron leaf, etc. Through water extracting, alcohol extracting, decompression concentrating, vacuum drying and other steps, the medicine composition is prepared into different pharmaceutically acceptable preparations. The medicine composition has obvious curative effect on the said diseases.

The invention discloses a Chinese herbal compound composition with a function of treating constipation and a preparation method thereof. The Chinese herbal compound composition is prepared from the following raw materials in parts by weight: 20-30 parts of atractylodes macrocephala, 15-20 parts of figwort root, 15-20 parts of dwarf lilyturf tuber, 5-10 parts of immature bitter orange, 10-15 parts of officinal magnolia bark, 5-10 parts of almonds, 10-15 parts of hemp seed and 10-15 parts of snake gourd seed. The Chinese herbal compound composition and the preparation method have the advantages that treatment based on syndrome differentiation is carried out according to the theory of the traditional Chinese medicine; scientific composition is carried out according to the pathogenesis of constipation; experimental study results prove that the Chinese herbal compound composition can excite smooth muscles of intestinal canals, increase the muscle tones, promote gastrointestinal motility and obviously increase the contraction amplitude, not only has the obvious functions of promoting small intestine movement and accelerating gastric emptying but also has the obvious functions of reducing the defecating time and increasing the defecating frequency. Therefore, the Chinese herbal compound composition provided by the invention is expected to be developed into a new-generation safe and effective new medicine for treating constipation.

The invention discloses a method of improving an antioxidant activity of walnut cake protein. The method of improving the antioxidant activity of the walnut cake protein includes the following steps of: 1, testing influences of enzyme types on a walnut cake protein yield; 2, testing influences of enzyme addition amounts on the walnut cake protein yield; 3, testing influences of material-liquid ratios on the walnut cake protein yield; 4, testing influences of grain sizes on the walnut cake protein yield; 5, testing influences of extraction time on the walnut cake protein yield; 6, measuring thecontent of the protein in an extraction solution; 7, conducting enzyme-assistant walnut cake protein extracting response surface experiments; and 8, analyzing the influences of the enzyme types on the walnut cake protein yield. By adopting the method of improving the antioxidant activity of the walnut cake protein, the problems are solved that current methods cannot be applied to the fields of food and drugs, during experiments of the current methods, temperature control is involved, the single-factor cost is high, a safety factor is low, the antioxidant activity of the prepared walnut cake protein is extremely low, and through a buffer solution, a high protein yield cannot be reached.

The invention relates to an immune potentiator based on hirsutella sinensis and a preparation method thereof. The potentiator is white dried powder and mainly composed of saccharides, amino acids andderivatives thereof and nucleosides compounds, and the potentiator is provided with an immune enhancing effect, can improve the immunity of the body and has a broad market. The culturing of the hirsutella sinensis is optimized by methods of adding metabolism induction materials and the like to allow the immunity active substances in a cultured substance to be increased; the optimal extracting technology of the immunity active substances in the obtained hirsutella sinensis is obtained through extracting and the optimization of a spectrum separation preparation technology. The immunity active substances are mainly obtained from a hirsutella sinensis solution, and bi-produc thirsutella sinensis filaments can replace related health protection products of wild cordyceps sinensis processing, anddual-purpose is achieve. According to the method, the immune potentiator is produced through indoor fermentation, the industrial production of the immune potentiator can be conducted all year round,the production is not influenced by severe environmental factors of plateau low temperature of the wild cordyceps and the like, and the ecological environment is not damaged.

The invention relates to a traditional Chinese medicinal composition for relieving physical fatigue. The traditional Chinese medicinal composition for relieving the physical fatigue is prepared from the following bulk pharmaceutical chemicals: cornua cervi pantotrichum, ginseng, eucommia bark, morinda officinalis, barbary wolfberry fruit, cinnamon, cape jasmine and fructus amomi. Part of medicinal materials are smashed in a certain ratio, the rest of the medicinal materials are extracted, concentrated, dried and smashed, and auxiliary materials are added, so that the traditional Chinese medicinal composition for relieving the physical fatigue is obtained.

The invention discloses a monascus mycelium resource comprehensive utilization method. According to the monascus mycelium resource comprehensive utilization method, ultrasonic assistant extraction is adopted to extract monascus pigment in monascus mycelium, optimum extraction conditions of monascus pigment are determined, extraction rate can be increased to 94.01%; acid and alkali treatment is adopted, optimum extraction conditions of crude protein are determined, and extraction rate is increased to 64.3%; direct alkali adding is adopted, optimum extraction conditions of ergosterol are determined, extraction rate is increased to 4.16%; and alkali adding extraction is adopted, optimum extraction conditions of chitosan are determined, and chitosan yield is 2.45%. The monascus mycelium resource comprehensive utilization method is capable of maximizing the utilization of resources, and achieving relatively high economic, social, and ecological benefits.

The invention discloses a method for extracting taurine from an octopus liver. The method for extracting the taurine from the octopus liver comprises the steps that methods of pretreatment, self-digestion, extraction, deacidification, de-basic protein, decoloration concentration, ion exchange adsorption, eluant collection and ethyl alcohol and recrystallization addition are carried out on the octopus liver to obtain a taurine pure product. According to the method for extracting the taurine from the octopus liver, the taurine extraction rate is used as an index, the taurine is extracted from the octopus liver by adopting a water-boiling method, the influence of four factors of feed liquid ratio, extraction temperature, extraction time and self-digestion time on the taurine extraction rate is studied, and an optimum extraction process is optimized through orthogonal experiment. The preparation process has no pollution, the process is simple and easy to operate, the cost is lower, resin is reproducible, the obtained product purity is high, and good practical significance and application prospects are achieved.

The invention provides a preparation method for sargassum confusum C.Ag. phlorotannins. The preparation method comprises the steps: evenly mixing dried sargassum confusum C.Ag. and a 40 vol% ethanol solution with the amount 20 times that of the dried sargassum confusum C.Ag., carrying out water bath extraction for 46 min at the temperature of 30 DEG C, centrifuging the extract liquid for 10 min at the speed of 4000 r / min, repeating extraction for 2 times, and thus obtaining a phlorotannins extract liquid. The method for optimizing the sargassum confusum C.Ag. phlorotannins best extraction conditions by a response surface method is used, and 3 independent variables comprising the ethanol volume fraction, the material-to-liquid ratio and the extraction time are set; screening is performed by using a Box-Benhnken response surface design principle; experimental design and result analysis are performed by using Design expert software; the exaction technology of the sargassum confusum C.Ag. phlorotannins improves the extraction rate of the phlorotannins, and plays an important role in industrialized production of the phlorotannins.

The invention discloses a kidney tonifying and life lengthening tablet preparation technology. A formula of the kidney tonifying and life lengthening tablet is prepared from the following raw materials in parts by weight: 120 to 130 parts of radix ginseng rubra, 4.0 to 5.0 parts of pearl powder, 310 to 320 parts of lucid ganoderma, 625 to 635 parts of prepared radix polygoni multiflori, 625 to 635 parts of fructus lycii, 940 to 950 parts of herba epimedii, 310 to 320 parts of radix salvia miltiorrhizae, 120 to 130 parts of liquorice root and 310 to 320 parts of rhizoma polygonati. The kidney tonifying and life lengthening tablet can be prepared through the steps of alcohol extracting the radix ginseng rubra and the herba epimedii, one step granulating through a fluidized bed, extracting materials, concentrating, mixing and drying. According to the kidney tonifying and life lengthening tablet preparation technology, effective ingredients of the radix ginseng rubra and the herba epimedii in the formula are fully utilized. The kidney tonifying and life lengthening tablet is small in loss of effective ingredients and stable in quality.

The invention relates to a salvianolic acid A capsule comprising by weight 5%-60% of a salvianolic acid A composition, 94.9%-39.5% of a filler and 0.1%-0.5% of a lubricant. The salvianolic acid A composition comprises salvianolic acid A more than 93% and less than 100%, and other 4 ingredients including 0.1%-2% of alkannic acid, 0.1%-2% of rosmarinic acid, 0.1%-2% of salvianolic acid B, 0.1%-2% of salvianolic acid C. The filler is one or more selected from starch, dextrin, sucrose, mannitol, microcrystalline cellulose, pregelatinized starch and calcium sulfate. The lubricant is one or more selected from magnesium stearate, talcum powder, superfine silica powder and polyethylene glycol. The invention also discloses application of the salvianolic acid A capsule to preparation of medicine for preventing and / or treating ischemic cerebrovascular disease.

The invention discloses a traditional Chinese medicine compound composition capable of treating infantile constipation and a preparation method thereof. The traditional Chinese medicine compound composition is prepared from 6-15 parts of immature bitter orange, 6-15 parts of magnolia officinalis, 6-15 parts of snakegourd root, 6-15 parts of fructus forsythiae, 6-15 parts of medicated leaven, 6-15parts of fried cassia seed, 3-5 parts of rhubarb, and 3-9 parts of chicken's gizzard-membrane. The traditional Chinese medicine compound composition provided by the invention provides treatment according to differentiation of symptoms and signs on the basis of the theory of traditional Chinese medicine and has a scientific prescription according to the pathogenesis of infantile constipation; and experimental research results prove that the traditional Chinese medicine compound composition provided by the invention has scientific and reasonable proportion of each component, can obviously improve the activity of pepsin in rats, has a promoting effect on a propulsion function of small intestines in mice, and can reduce serum SS of the mice, and increase MTL and GAS, thereby regulating gastrointestinal hormones and promoting gastrointestinal peristalsis. Moreover, clinical experimental results show that the traditional Chinese medicine compound composition has a very good therapeutic effect on infantile constipation, and has good safety and no toxic or side effects.

The invention relates to a method for preparing taurine by high-voltage pulsed electric field assisted enzymolysis of freshwater mussel meat, and aims to solve the problem of lack of a method for extracting taurine from freshwater mussel meat at present. The high-purity natural taurine product is effectively extracted from freshwater mussel meat through the steps of raw material pretreatment, wallbreaking treatment, high-voltage pulsed electric field treatment, enzymolysis, filtration, purification, crystallization and the like, the production process is environmentally friendly and efficient, the test operation is simple, the taurine extraction effect is improved, the problems of freshwater mussel byproduct resource waste, environment pollution and the like are solved, the taurine extraction amount is used as an index, the four factors of the electric field intensity, the pulse number, the enzyme addition amount and the enzymolysis time are optimized, and the taurine extraction amount of freshwater mussel meat is greatly increased.

The invention discloses a traditional Chinese medicinal compound composition for treating neurogenic bladder disorders. The composition is prepared from the following materials: 10 to 30 parts of coix seeds, 10 to 30 parts of scrophularia ningpoensis, 10 to 30 parts of astragalus membranaceus, 10 to 30 parts of atractylodes macrocephala, 10 to 30 parts of cornus officinalis, 10 to 30 parts of mantis egg case, 10 to 30 parts of rhizoma acori graminei, 2 to 10 parts of manis pentadactyla, 5 to 15 parts of alisma plantago-aquatica, 5 to 25 parts of semen plantaginis, 10 to 30 parts of rhizoma dioscoreae colletii, 10 to 30 parts of dried pyrrosia lingua leaves, 10 to 30 parts of tetrapanax papyriferus, 10 to 30 parts of lysimachia christinae hance, 10 to 30 parts of radix stephaniae tetrandrae, 10 to 30 parts of dried laminaria sporophytes, 10 to 25 parts of dried pyrrosia lingua leaves and 10 to 30 parts of gardenia jasminoides. According to the invention, the raw material compatibility prescription is selected based on traditional Chinese medical theory, and the compatibility of all components is scientific and reasonable; experimental results show that the traditional Chinese medicinal compound composition can effectively improve urinary tract symptoms and urodynamic related indicators, effectively restore the lower urinary tract function and reduce the bladder residual urine volume, and has a good curative effect on neurogenic bladder disorders.

The invention discloses a method for extracting dietary fibre of Auricularia nigricans by using an alkaline process, belongs to the field of extraction of plant dietary fibre, and aims at providing a method for extracting the dietary fibre of the Auricularia nigricans by using the alkaline process and acquiring a best extraction technology. The method specifically comprises the following steps of pre-processing the Auricularia nigricans; adding a sodium hydroxide solution into the pre-processed Auricularia nigricans until the material liquid ratio is (1:20) to (1:280); and performing extracting on the processed Auricularia nigricans under extracting temperature of 60 DEG C to 100 DEG C to obtain Auricularia nigricans slag powder.

The invention relates to arachis hypogaea L. nerve-soothing oral liquid and a preparing method and application thereof. The preparing method of the arachis hypogaea L. nerve-soothing oral liquid comprises the following steps of taking ramulus et folium arachis hypogaea L., adding 10-fold water to conduct soaking for 2-4 h, conducting decocting twice, and adding 10-fold water for the second time, wherein decocting is conducted for 1 h every time; filtering decoction liquid, merging filtrates to be concentrated to the relative density of 1.15-1.20, chilling, adding ethyl alcohol to make the alcohol content reach 60%, uniformly stirring the mixture, standing for 24 h, filtering to obtain liquid supernatant, recycling ethyl alcohol in the filtrate till the filtrate has no smell of alcohol, adding water and uniformly stirring the filtrate, standing for 24 h, taking the liquid supernatant to be filtered, adding distillate, saccharose and sodium benzoate to be dissolved, adjusting the relative density to 1.07-1.10, conducting cold storage and standing for 48 h, filtering, adding water to the filtrate, boiling under the sealed condition, and filling and sterilizing to obtain the arachis hypogaea L. nerve-soothing oral liquid. By means of the arachis hypogaea L. nerve-soothing oral liquid and the preparing method and application thereof, active components of ramulus et folium arachis hypogaea L.for treating anhypnia are sifted and serve as indexes, and the optimum preparing technology of the arachis hypogaea L. nerve-soothing oral liquid preparation is sifted out.

The invention discloses a mechanochemical extraction method for preparing a naphthopyranone crude extract from berchemia lineata, which comprises the following steps: drying, crushing and sieving a berchemia lineata raw material, putting the obtained powder into a ball mill for ball milling, mixing the ball-milled powder with an extraction solvent, then putting the mixture into a water bath at a temperature between 20 and 50 DEG C for ultrasonic extraction for 15 to 60 minutes, then cooling to room temperature, filtering, and obtaining a yellow brown naphthopyranone crude extract after the solvent is removed through rotating evaporation of filtrate; dissolving the crude extract with methanol, standing, collecting supernatant, sieving by using a PTFE membrane of 0.22 micrometer; and analyzing by using an UPLC method, the yield of the crude extract is obtained by using the content of the main component of the naphthopyranone crude extract which is naphthopyranone-6-O-alpha-L-rhamnosyl-(1-6)-O-beta-D glucopyranoside. According to the invention, the method is low in cost; the process for preparing and extracting the crude extract of the naphthopyranone is rapid and efficient; and technical basis can be provided for the comprehensive utilization of the medicinal resources of the berchemia lineata.

The invention discloses an optimal extraction process of polyphenol in tinder fungus. The optimal extraction process comprises the following steps: single-factor experiment, response surface design, determination of optimal extraction conditions of polyphenol in the tinder fungus, and determination of crude extract polyphenol content,. wherein the single-factor experiment comprises drawing of a standard curve, influence of ethanol concentration on extraction of tinder fungus polyphenol, influence of material-liquid ratio on extraction of the polyphenol in the tinder fungus, influence of ultrasonic time on extraction of the polyphenol in the tinder fungus, influence of ultrasonic temperature on extraction of the polyphenol in the tinder fungus, influence of extraction times on extraction ofthe polyphenol in the tinder fungus, and multiple experimental principle steps. According to the optimal extraction process of the polyphenol in the tinder fungus, the influence of the ethanol concentration, the material-liquid ratio, the ultrasonic time, the ultrasonic temperature and the extraction frequency on extraction of the polyphenol in the tinder fungus is determined by designing a single-factor experiment, and the optimal extraction process is obtained by designing a response surface. Reference is provided for development and utilization of the polyphenol in the tinder fungus.

The invention relates to a traditional Chinese medicinal composition for relieving physical fatigue. The traditional Chinese medicinal composition for relieving the physical fatigue is prepared from the following bulk pharmaceutical chemicals: cornua cervi pantotrichum, ginseng, eucommia bark, morinda officinalis, barbary wolfberry fruit, cinnamon, cape jasmine and fructus amomi. Part of medicinal materials are smashed in a certain ratio, the rest of the medicinal materials are extracted, concentrated, dried and smashed, and auxiliary materials are added, so that the traditional Chinese medicinal composition for relieving the physical fatigue is obtained.

The invention relates to an extraction condition optimization method and an extraction method of tripterygium multi-index active ingredients. The extraction condition optimization method is characterized in that against optimal extraction of the tripterygium multi-index active ingredients, an ultrasonic extraction process of the tripterygium active ingredients in the prior art is integrated, through single-factor experimental investigation, three factors, namely liquid-to-solid ratio, solvent concentration and ultrasonic power, which have relatively great effects on extraction overall desirability value (OD value) of the tripterygium multi-index active ingredients, are selected, design expert software is utilized, and a star point design-response surface optimization method is applied for optimization, so that optimal extraction process parameters are obtained. The methods provided by the invention are simple, the experimental precision is high, the optimal values are calculated by model fitting, resources and energy consumption are saved, and a guide can be provided for industrialized extraction and production.

The invention discloses a method for extracting melanin of morchella esculenta through cellulase and ultrasonic wave synergism. The method comprises the following steps: (1) crushing morchella esculenta sporocarp, dissolving the crushed morchella esculenta sporocarp in water, and adding cellulase for enzymolysis; (2) carrying out ultrasonic treatment on the system after enzymolysis; (3) adding alkali into the system subjected to ultrasonic treatment for alkali extraction, centrifuging after the alkali extraction is finished, and collecting supernate to obtain a melanin extracting solution; adding acid to carry out acid precipitation, cleaning the collected precipitate, and drying to obtain a melanin crude product; (4) dissolving the melanin crude product in water, adding protease for hydrolysis to remove protein, and then removing grease; and adding an acid for acid precipitation, cleaning the collected precipitate, and drying to obtain the morchella esculenta melanin. The optimal extraction process of the melanin of the morchella esculenta is obtained through a single factor test, a PB test and a response surface method, and the optimal deproteinization method is screened by taking the melanin yield and the protein content as indexes.

The invention discloses a method for determining content of hesperidin in dried tangerine or orange peel by virtue of an ASE-HPLC (accelerated solvent extraction-high performance liquid chromatography) process and belongs to the field of chemical detection. The method is short in extraction time and high in detection accuracy. The method disclosed by the invention adopts an ASE process for extracting dried tangerine or orange peel powder, and collecting methanol extract liquor; and determining the content of hesperidin in the methanol extract liquor by adopting an HPLC process. The method disclosed by the invention can replace a pharmacopeia method for extracting the hesperidin in the dried tangerine or orange peel and determining the content of hesperidin.