Method for preparing taurine by high-voltage pulsed electric field assisted enzymolysis of freshwater mussel meat

A high-voltage pulsed electric field, assisted enzymatic hydrolysis technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of instability, extraction method of taurine, low extraction rate, etc. Waste, green and efficient production process, improved extraction effect

Active Publication Date: 2020-09-18
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although there are methods for extracting taurine from meat products and seafood in the prior art, there is no special extraction method for extracting taurine from river mussels, and there is a technical gap. If the existing extraction technology is directly applied to In the process of extracting taurine from mussel meat, the extraction rate is low and unstable, so many parameters and steps in the extraction process need to be further innovated, optimized and improved

Method used

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  • Method for preparing taurine by high-voltage pulsed electric field assisted enzymolysis of freshwater mussel meat
  • Method for preparing taurine by high-voltage pulsed electric field assisted enzymolysis of freshwater mussel meat
  • Method for preparing taurine by high-voltage pulsed electric field assisted enzymolysis of freshwater mussel meat

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Experimental program
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Effect test

Embodiment 1

[0029] A high-voltage pulsed electric field-assisted enzymatic hydrolysis method for producing taurine, comprising the following steps:

[0030] (1) Raw material pretreatment:

[0031] Shell the clam meat to separate the viscera and meaty part, wash the meaty part, drain and homogenize it, and distribute it for equipment; (2) Wall breaking treatment:

[0032] Add distilled water to the pretreated mussel meat homogenate, the amount of distilled water added and the quality of the sample solution are added according to 1:1 (mL / g), and the mussel meat sample solution is obtained after intermittent treatment with a wall breaking machine for 2-3min. For distributing equipment;

[0033] (3) High-voltage pulsed electric field treatment:

[0034] Take 4g of mussel meat sample liquid, add 32mL of distilled water to the sample liquid for homogenization, adjust the flow rate parameter of the peristaltic pump to 7mL / min after homogenization, and then perform pulse electric field treatmen...

Embodiment 2

[0047] A high-voltage pulsed electric field-assisted enzymatic hydrolysis method for producing taurine, comprising the following steps:

[0048] (1) Raw material pretreatment:

[0049] Shell the clam meat to separate the viscera and meaty part, wash the meaty part, drain and homogenize it, and distribute it for equipment; (2) Wall breaking treatment:

[0050] Add distilled water to the pretreated mussel meat homogenate, the amount of distilled water added and the quality of the sample solution are added according to 1:1 (mL / g), and the mussel meat sample solution is obtained after intermittent treatment with a wall breaking machine for 2-3min. For distributing equipment;

[0051] (3) High-voltage pulsed electric field treatment:

[0052] Take 4g mussel meat sample liquid, add 32mL distilled water to the sample liquid for homogenization, adjust the flow rate parameter of the peristaltic pump to 7mL / min after homogenization, and then perform pulse electric field treatment, pul...

Embodiment 3

[0065] A high-voltage pulsed electric field-assisted enzymatic hydrolysis method for producing taurine, comprising the following steps:

[0066] (1) Raw material pretreatment:

[0067] Shell the clam meat to separate the viscera and meaty part, wash the meaty part, drain and homogenize it, and distribute it for equipment; (2) Wall breaking treatment:

[0068] Add distilled water to the pretreated mussel meat homogenate, the amount of distilled water added and the quality of the sample solution are added according to 1:1 (mL / g), and the mussel meat sample solution is obtained after intermittent treatment with a wall breaking machine for 2-3min. For distributing equipment;

[0069] (3) High-voltage pulsed electric field treatment:

[0070] Take 4g of mussel meat sample liquid, add 32mL of distilled water to the sample liquid for homogenization, adjust the flow rate parameter of the peristaltic pump to 7mL / min after homogenization, and then perform pulse electric field treatmen...

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Abstract

The invention relates to a method for preparing taurine by high-voltage pulsed electric field assisted enzymolysis of freshwater mussel meat, and aims to solve the problem of lack of a method for extracting taurine from freshwater mussel meat at present. The high-purity natural taurine product is effectively extracted from freshwater mussel meat through the steps of raw material pretreatment, wallbreaking treatment, high-voltage pulsed electric field treatment, enzymolysis, filtration, purification, crystallization and the like, the production process is environmentally friendly and efficient, the test operation is simple, the taurine extraction effect is improved, the problems of freshwater mussel byproduct resource waste, environment pollution and the like are solved, the taurine extraction amount is used as an index, the four factors of the electric field intensity, the pulse number, the enzyme addition amount and the enzymolysis time are optimized, and the taurine extraction amount of freshwater mussel meat is greatly increased.

Description

technical field [0001] The invention relates to a method for preparing taurine, in particular to a method for preparing taurine through enzymolysis of mussel meat assisted by a high-voltage pulse electric field. Background technique [0002] Taurine, whose scientific name is β-aminoethanesulfonic acid, is a sulfur-containing amino acid that exists in various tissues of animals in the form of free amino acids, and has physiological activity and various nutritional functions. At present, there are two production methods of taurine: chemical synthesis and natural extraction. Due to the existence of toxic substances in the raw materials and production process of chemical synthesis, people advocate the use of green and safe natural extraction to extract taurine. [0003] Although there are methods for extracting taurine from meat products and seafood in the prior art, there is no special extraction method for extracting taurine from river mussels, and there is a technical gap. If...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C303/44C07C309/14
CPCC07C303/44C07C309/14
Inventor 周亚军翟时敏詹妮陈艳张鸣镝郑磊李圣桡
Owner JILIN UNIV
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