Preparation method for sargassum confusum C.Ag. phlorotannins

A technology of Haisongzi and ethanol solution, applied in the field of functional food or biomedicine, can solve problems such as unfavorable industrialized production, inability to popularize and use, and long experiment time.

Inactive Publication Date: 2016-03-30
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, several methods are not suitable for the drying of echinacea
Drying, air drying and microwave drying can easily cause the loss of polyphenols in sea spores; the drying speed of sea spunberries is slow due to the many microorganisms on the surface during the drying process; the freeze-drying has problems such as slow processing speed and high cost, and cannot Universal use
[0005] Microwave-assisted extraction and ultrasonic-assisted extraction can shorten the extraction time, the extraction rate is high, the operation is simple, the extraction structure is not easy to damage, but the cost is high and the energy consumption is large. The method commonly us

Method used

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  • Preparation method for sargassum confusum C.Ag. phlorotannins
  • Preparation method for sargassum confusum C.Ag. phlorotannins

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0019] Example 1.

[0020] 1. The extraction and content determination of the polyphenols of Kobresia japonica are carried out through the following steps.

[0021] 1. Sample preparation and pre-treatment: first use clean sea water to clean the impurities and sand on the surface of the collected fresh sea scorpion, soak in electrolyzed water to desalinate and reduce bacteria, then use 75% alcohol to scald and soak for 3-5S, and drain. . Spread the sea Songzi on a porcelain plate with a thickness of 2cm-4cm, and gently roll it with a roller for 3-5min. The sea Songzi should become wilted without juice. Set a cold air drying box, adjust the temperature to 20-30℃, wind speed 2-5m / s, humidity below 40%, dry with cold air until the moisture content is lower than 12-15%; adjust temperature to 5-10℃, wind speed 2-5m / s , The humidity is below 30%, and the cold air is dried until the moisture content is below 6%. Smash for later use.

[0022] 2. Weigh 0.2g of dried sea Songzi powder in a ...

Example Embodiment

[0038] Example 2.

[0039] A method for extracting polyphenols from Kobresia japonica, including the following steps:

[0040] Rinse the sea Songzi, soak in electrolyzed water to desalinate and reduce bacteria, and then use 75% alcohol to scald and soak for 4S, and drain. Spread the sea Songzi on a porcelain plate with a thickness of 3cm and gently roll it with a roller for 5 minutes. It is advisable that the sea Songzi becomes lush and does not produce juice. Set a cold air drying box, adjust the temperature to 25°C, wind speed 2m / s, humidity 40%, cold air drying to 12% moisture content; adjust the temperature to 10°C, wind speed 2m / s, humidity 30%, and cool air drying to 6% moisture content. The dried sea Songzi was crushed into coarse powder with a pulverizer, and then pulverized with a vibrating drug ultrafine pulverizer at -10°C for 12 minutes to obtain Hai Songzi powder. Take the above sea Songzi powder and add 20 times the volume fraction to 40% ethanol solution (that is, ...

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Abstract

The invention provides a preparation method for sargassum confusum C.Ag. phlorotannins. The preparation method comprises the steps: evenly mixing dried sargassum confusum C.Ag. and a 40 vol% ethanol solution with the amount 20 times that of the dried sargassum confusum C.Ag., carrying out water bath extraction for 46 min at the temperature of 30 DEG C, centrifuging the extract liquid for 10 min at the speed of 4000 r/min, repeating extraction for 2 times, and thus obtaining a phlorotannins extract liquid. The method for optimizing the sargassum confusum C.Ag. phlorotannins best extraction conditions by a response surface method is used, and 3 independent variables comprising the ethanol volume fraction, the material-to-liquid ratio and the extraction time are set; screening is performed by using a Box-Benhnken response surface design principle; experimental design and result analysis are performed by using Design expert software; the exaction technology of the sargassum confusum C.Ag. phlorotannins improves the extraction rate of the phlorotannins, and plays an important role in industrialized production of the phlorotannins.

Description

technical field [0001] The invention relates to the field of functional food or biomedicine, in particular to a preparation method of eruption polyphenols. Background technique [0002] Sargassum confusum C.Ag. is a plant that grows in warm temperate waters and is rich in coastal resources in my country. Jellyfish is rich in bioactive substances and has high health care and medicinal value. Artemisia polysaccharides have toxic and side effects on leukemia cell lines and can inhibit the proliferation of tumor cells. Fucoidan is also one of the main active ingredients. Modern studies have shown that fucoidan has various physiological functions such as anti-tumor, anti-virus, anti-thrombosis, anti-oxidation, blood lipid regulation, anti-radiation and inhibition of complement activation. Our country is rich in Echinacea resources, but due to the late start of the research on the comprehensive development of Echinacea, most of the research on its biological activity is concentr...

Claims

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Application Information

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IPC IPC(8): A61K36/03A23L33/105
CPCA23V2002/00A61K36/03A61K2236/13A61K2236/15A61K2236/17A61K2236/33A61K2236/333A61K2236/39A61K2236/53A23V2200/30A23V2250/2132
Inventor 刘智禹吴靖娜熊何健苏永昌蔡水淋许旻刘淑集苏捷潘南
Owner FISHERIES RES INST OF FUJIAN
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