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Method for extracting Morchella esculenta melanin through cellulase and ultrasonic wave synergism

A technology for extracting morel melanin and its extraction method, which is applied in the field of melanin extraction and cellulase ultrasonic collaborative extraction of melanin from morel melanin, which can solve the problems of heterogeneity, low efficiency, and difficulty in melanin

Active Publication Date: 2022-03-22
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the course of the test, it takes a long time and the efficiency is low to use the traditional alkali-dissolving and acid-precipitating method.
In addition, because melanin is easy to combine with proteins, oils and polysaccharides, as well as its heterogeneity and inhomogeneity, it is difficult to purify melanin

Method used

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  • Method for extracting Morchella esculenta melanin through cellulase and ultrasonic wave synergism
  • Method for extracting Morchella esculenta melanin through cellulase and ultrasonic wave synergism
  • Method for extracting Morchella esculenta melanin through cellulase and ultrasonic wave synergism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 explored the effects of different protein removal methods on extraction yield and protein content; Examples 2-7 explored different NaOH solution concentrations, different cellulase additions, different cellulase enzymatic hydrolysis times, and different Effects of cellulase enzymatic hydrolysis temperature, solid-liquid ratio, and ultrasonic time on melanin yield (single factor); each treatment of the above single factor experiment was repeated three times, and the test results were analyzed by variance with SPSS 17.0 software, and P<0.05 indicated Significant difference. Embodiment 8 is on the basis of single factor test, carries out Plackett-Burman test design to 6 single factors, takes yield as judgement index, has screened out the most significant influence factor that influences the yield of melanin of Morchella melanogaster; According to The optimal result of the Plackett-Burman experimental design was used as the center point, and the yield was the resp...

Embodiment 2

[0081] Example 2, Cellulase Ultrasonic Cooperative Extraction of Morchella melanin

[0082] Follow the steps below to extract the melanin from Morchella edulis:

[0083] (1) Accurately weigh 5.00g of Morchella versicolor fruit body powder and dissolve it in 20mL of distilled water, add cellulase for enzymolysis, the amount of cellulase added is 8mg / g, the enzymolysis temperature is 40°C, and the enzymolysis time is 100min;

[0084] (2) After the enzymolysis is completed, the system after the enzymolysis is subjected to ultrasonic treatment, the ultrasonic power is 200W, and the time is 80min;

[0085] (3) After the ultrasound is finished, add NaOH solution to the system after ultrasound to prepare 150mL NaOH solutions with concentrations of 0.5, 1.0, 1.5, 2.0, and 2.5mol / L (the ratio of solid to liquid is 1g: 30mL), and bathe in water at 60°C for 2h Carry out alkaline extraction. After the alkaline extraction, centrifuge at 10000r / min for 5min, collect the supernatant to ob...

Embodiment 3

[0088] Example 3, Cellulase Ultrasonic Synergistic Extraction of Melanin from Morchella melanum

[0089] Follow the steps below to extract the melanin from Morchella edulis:

[0090] (1) Accurately weigh 5.00g of Morchella versicolor fruit body powder and dissolve it in 20mL of distilled water, and add fiber according to the cellulase dosage of 8mg / g, 12mg / g, 16mg / g, 20mg / g, and 24mg / g respectively enzymatic hydrolysis with cellulase, the enzymatic hydrolysis temperature of cellulase is 40°C, and the enzymatic hydrolysis time is 100min;

[0091] (2) After the enzymolysis is completed, the system after the enzymolysis is subjected to ultrasonic treatment, the ultrasonic power is 200W, and the ultrasonic time is 80min;

[0092] (3) After the ultrasonication, NaOH solution was added to the ultrasonicated system to make 150mL NaOH solution with a concentration of 1.5mol / L (the ratio of solid to liquid was 1:30), and alkaline extraction was carried out in a 60°C water bath for 2h....

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Abstract

The invention discloses a method for extracting melanin of morchella esculenta through cellulase and ultrasonic wave synergism. The method comprises the following steps: (1) crushing morchella esculenta sporocarp, dissolving the crushed morchella esculenta sporocarp in water, and adding cellulase for enzymolysis; (2) carrying out ultrasonic treatment on the system after enzymolysis; (3) adding alkali into the system subjected to ultrasonic treatment for alkali extraction, centrifuging after the alkali extraction is finished, and collecting supernate to obtain a melanin extracting solution; adding acid to carry out acid precipitation, cleaning the collected precipitate, and drying to obtain a melanin crude product; (4) dissolving the melanin crude product in water, adding protease for hydrolysis to remove protein, and then removing grease; and adding an acid for acid precipitation, cleaning the collected precipitate, and drying to obtain the morchella esculenta melanin. The optimal extraction process of the melanin of the morchella esculenta is obtained through a single factor test, a PB test and a response surface method, and the optimal deproteinization method is screened by taking the melanin yield and the protein content as indexes.

Description

technical field [0001] The invention relates to a method for extracting melanin, in particular to a method for cooperating with cellulase and ultrasonic waves to extract melanin from Morchella spp., belonging to the technical field of edible fungus deep processing. Background technique [0002] Morchella, commonly known as goat sparrow, honeycomb mill or morel, belongs to Ascomycotina, Discomycetes, Pezizales, Morchellacea, Morchella ( Morchella). Morels are roughly divided into three branches according to the color of the fruiting bodies, namely red morels, yellow morels, and black morels. Morchella importuna belongs to the branch of black morel. M.importuna is rich in amino acids, polysaccharides, melanin, saponins, ketones, aldehydes, esters and other active substances needed by the human body. Enhance the role of human immunity, and is the main strain of artificial cultivation in my country. [0003] Melanin is a common biological pigment. It is a biological macromole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 徐丽婧孟俊龙常明昌刘文婷冯翠萍程艳芬耿雪冉王昭玉曹群珞
Owner SHANXI AGRI UNIV
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