Method for extracting taurine from octopus liver

A taurine, liver technology, applied in chemical instruments and methods, pharmaceutical formulations, preparations for skin care, etc.

Inactive Publication Date: 2019-05-17
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for extracting taurine from octopus liver
  • Method for extracting taurine from octopus liver
  • Method for extracting taurine from octopus liver

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The single factor test that embodiment 1 taurine extracts

[0030] (1) The effect of solid-liquid ratio on the extraction rate of taurine from octopus liver:

[0031] Five groups of about 25.0g octopus liver homogenate were respectively subjected to autolysis time of 24h, extraction temperature of 70°C, and extraction time of 40min, and the ratio of solid to liquid was 1:3g / mL, 1:4g / mL , 1: 5g / mL, 1: 6g / mL, 1: 7g / mL on the taurine extraction yield of octopus liver.

[0032] The effect of solid-liquid ratio on the extraction yield of taurine in the single factor test is as follows: figure 1 shown. With the continuous increase of solid-liquid ratio, the extraction rate of taurine in octopus liver showed a trend of first increasing and then gradually decreasing. When the solid-liquid ratio was 1:4, the extraction rate was the highest at 3.63 mg / g, which was significant. The extraction yield of taurine is higher than other solid-liquid ratios. After that, the solid-liqu...

Embodiment 2

[0042] The orthogonal experiment that embodiment 2 taurine extracts

[0043] According to the results of the single factor test, the main factors and levels of this experiment were screened, and the L 9 (3 4 ) Orthogonal test table to determine the optimal combination of process conditions for the extraction of octopus liver taurine, the levels of orthogonal factors are shown in Table 1. The test results were collated by Office 2007, and SPSS 19.0 statistical software was used to conduct variance analysis on the data, construct F statistics, and perform F test to judge the significance of the factors.

[0044] Table 1 Orthogonal test factor level table

[0045]

[0046] According to the single factor test results, four influencing factors and their levels were selected, including solid-liquid ratio, extraction time, extraction temperature, and autolysis time, and the extraction rate of taurine from octopus liver was used as an index, and the L 9 (3 4 ) Orthogonal test t...

Embodiment 3

[0053] Example 3 Extraction and detection of octopus liver taurine

[0054] 1) Experimental materials and reagents

[0055] Octopus liver: Tangshan Fengrui Aquatic Food Co., Ltd.;

[0056] Taurine standard sample (99.9%): Shanghai Luyuan Technology Co., Ltd.;

[0057] 732Na + Type cation exchange resin: Beijing Solaibao Technology Co., Ltd.;

[0058] Activated carbon: Tianjin Tianli Co., Ltd.;

[0059] Other reagents are commonly used reagents in the laboratory.

[0060] 2) Extraction step

[0061] (1) Sample pretreatment: After washing the fresh octopus, take out about 25.0 g of the liver, and put it in a tissue masher to homogenize the tissue at a ratio of 1:4 between the liver and distilled water (g / mL) until it becomes minced;

[0062] (2) Autolysis: put the homogenate in a 50°C water bath, plug the mouth of the bottle with cotton, and autolyze for 22 hours;

[0063] (3) Extraction: Adjust the pH of the solution to 4.5, place it in a water bath at 80°C for 50 minute...

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Abstract

The invention discloses a method for extracting taurine from an octopus liver. The method for extracting the taurine from the octopus liver comprises the steps that methods of pretreatment, self-digestion, extraction, deacidification, de-basic protein, decoloration concentration, ion exchange adsorption, eluant collection and ethyl alcohol and recrystallization addition are carried out on the octopus liver to obtain a taurine pure product. According to the method for extracting the taurine from the octopus liver, the taurine extraction rate is used as an index, the taurine is extracted from the octopus liver by adopting a water-boiling method, the influence of four factors of feed liquid ratio, extraction temperature, extraction time and self-digestion time on the taurine extraction rate is studied, and an optimum extraction process is optimized through orthogonal experiment. The preparation process has no pollution, the process is simple and easy to operate, the cost is lower, resin is reproducible, the obtained product purity is high, and good practical significance and application prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of utilization of octopus processing by-products, and in particular relates to a method for extracting taurine from octopus liver. Background technique [0002] Taurine is a sulfur-containing non-protein amino acid that exists in a free state in the human body and has a variety of physiological functions. Taurine can promote the metabolism of proteins, lipids, amino acids and sugars, and can promote Cu, Absorption of Fe, Zn, Mn and other mineral elements and indirectly regulates lipid metabolism. Taurine has the function of protecting lymphocytes, and is also closely related to the total number of white blood cells in the blood. The lack of taurine may even lead to abnormal cell morphology and function. The study also found that taurine improved the immune regulation and antioxidant capacity of immunosuppressed mice of different ages, and had a significant effect on the reproductive performance, immune syst...

Claims

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Application Information

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IPC IPC(8): C07C309/14C07C303/44A23L2/52A23L33/175A61K8/46A61Q19/00
Inventor 桑亚新王向红于文龙刘卫华孙记录王雪静
Owner HEBEI AGRICULTURAL UNIV.
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