Wild chrysanthemum extraction process and measuring method for total flavonoids in wild chrysanthemums
An extraction process, the technology of wild chrysanthemum, which is applied in the fields of medical formula, color/spectral characteristic measurement, plant raw materials, etc., can solve the problems that there are not many research reports on the extraction and separation and purification process of total flavonoids from wild chrysanthemum, and there has not been in-depth and comprehensive research. , to achieve the effect of low raw material cost, high desorption rate and large adsorption capacity
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[0049] 1. Example of extraction
[0050] ①, ethanol extraction
[0051] Add 60% ethanol (volume percentage, the same below) to the dried chrysanthemum powder, heat to reflux, and extract 3 times at 70°C for 3 hours each time. The weight ratio is 10:1 to obtain ethanol extract;
[0052] ②, the first concentration and filtration
[0053] Concentrate the ethanol extract under reduced pressure, recover the ethanol under reduced pressure, and filter while hot;
[0054] ③, resin adsorption
[0055] The ethanol concentrate is adsorbed in the AB-8 type macroporous adsorbent resin, the weight ratio of the AB-8 type macroporous adsorbent resin and the ethanol concentrate is 7: 1, after the adsorption is completed, use 70% ethanol (volume percentage, the same below) ) elution, the weight ratio of 70% ethanol and ethanol concentrated liquid is 12:1, and then reclaims the ethanol eluent;
[0056] ④, the second concentration
[0057] Concentrating the ethanol eluent again under reduce...
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