A kind of method for extracting dietary fiber of Auricularia japonica by alkali method
A technology of dietary fiber and alkaline extraction, applied in food science and other directions, can solve the problems of high cost and low extraction efficiency
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specific Embodiment 1
[0027] Alkaline Extraction of Jade Fungus Dietary Fiber
[0028] Single factor method:
[0029] 1 Experimental materials
[0030] Jade fungus from Jilin Agricultural University, dehydrated, desugared and dried, crushed and sieved for use; 95% ethanol, absolute ethanol, deionized water and other reagents and solvents were of analytical grade. Acetic acid, 0.5%, 1.0%, 1.5%, 2.0%, 2.5% NaOH solution.
[0031] Experimental equipment: HH-4 electric heating constant temperature water bath; SHB-Ⅲ circulating water multi-purpose vacuum pump, 101-1 electric blast drying oven; electronic analytical balance; cooking and sterilizing pot.
[0032] 2 Fungus sample preparation
[0033] 2.1 Dehydration
[0034] Take 10 g of pulverized jade fungus, place it in a vacuum oven at 70°C ± 1°C, and dry it to constant weight. Transfer the dried sample to a desiccator, and weigh (mD) after the temperature of the sample drops to room temperature. According to the mass of the sample before and aft...
specific Embodiment 2
[0071] 1. Response surface results
[0072] 1. Central composite experimental design factors
[0073]
[0074] 2. Design results of central composite experiment
[0075]
[0076] 3. Analysis of variance of water-soluble dietary fiber
[0077]
[0078] According to Table 3, adopt the response analysis method to analyze the test results, obtain the regression equation with the water-soluble dietary fiber extraction amount as the response value:
[0079] Water-soluble dietary fiber SDF extraction amount=0.36+0.005833A+0.0025B-0.0008333C-0.004167D-0.0025AB+0.005AC-0.02AD+0.0025BC+0.012BD+0CD-0.037A 2 -0.044B 2 -0.0065C 2 -0.011D 2
[0080] 4. Analysis of variance of insoluble dietary fiber
[0081]
[0082] According to Table 4, adopt the response analysis method to analyze the test results, obtain the regression equation with the insoluble dietary fiber extraction amount as the response value:
[0083] Insoluble dietary fiber IDF =0.57-0.0025A+0.003333B-0.0066...
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