Immune potentiator based on hirsutella sinensis and preparation method thereof

Abstract

The invention relates to an immune potentiator based on hirsutella sinensis and a preparation method thereof. The potentiator is white dried powder and mainly composed of saccharides, amino acids andderivatives thereof and nucleosides compounds, and the potentiator is provided with an immune enhancing effect, can improve the immunity of the body and has a broad market. The culturing of the hirsutella sinensis is optimized by methods of adding metabolism induction materials and the like to allow the immunity active substances in a cultured substance to be increased; the optimal extracting technology of the immunity active substances in the obtained hirsutella sinensis is obtained through extracting and the optimization of a spectrum separation preparation technology. The immunity active substances are mainly obtained from a hirsutella sinensis solution, and bi-produc thirsutella sinensis filaments can replace related health protection products of wild cordyceps sinensis processing, anddual-purpose is achieve. According to the method, the immune potentiator is produced through indoor fermentation, the industrial production of the immune potentiator can be conducted all year round,the production is not influenced by severe environmental factors of plateau low temperature of the wild cordyceps and the like, and the ecological environment is not damaged.

Description

technical field

[0001] The invention belongs to the technical field of biological products and preparation, and in particular relates to an immune enhancer based on Mortierella sinensis and a preparation method. Background technique

[0002] Cordyceps sinensis is a well-known traditional Chinese herbal medicine in my country. It has been used for more than a thousand years. It is as famous as ginseng and antler. It is a complex of fungi formed by Cordyceps sinensis and parasitic insects, including the insect body part full of hyphae (sclerotia) and the fruiting body part grown from the head. Modern pharmacological research shows that Cordyceps sinensis can regulate immunity, delay aging, lower blood pressure, regulate endocrine, eliminate fatigue, prevent and treat tumors, acute or chronic hepatitis, nephritis, antibacterial and antiviral and other pharmacological activities, and has important medical and health care value . Due to the special growth environment of natural...

Examples

Embodiment 1

[0038] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:

[0039] 1) Strain selection

[0040] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );

[0041] 2) Strain culture

[0042] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:

[0043] 2.1) Preparation of primary solid bacteria

[0044]The isolated and purified slant strains were inoculated on the solid medium of a 9 cm petri dish, the medium was: 200.0 g potato juice, 20.0 g glucose, 10.0 g maltose, 10.0 g peptone, 10.0 g yeast powder, MgSO 4 1.5 g, KH2PO 4 Add 3.0 g of agar, 20.0 g of agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0 M NaOH; put it in an incubator at 17°C for 20 days to obtain a first-grade solid strain.

[0045] ) secondary liquid seed culture

[0046] Put 200ml of liquid medium in a 500mL conical flask; each 10...

Embodiment 2

[0055] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:

[0056] 1) Strain selection

[0057] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );

[0058] 2) Strain culture

[0059] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:

[0060] 2.1) Preparation of primary solid bacteria

[0061] The isolated and purified strains were inoculated on the solid medium of a 9 cm petri dish. The medium was: 200.0 g potato juice, 20.0 g glucose, 10.0 g maltose, 10.0 g peptone, 10.0 g yeast powder, MgSO 4 1.5 g, KH2PO 4 3.0 g, 20.0 g agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0 M NaOH; put it in a 20°C incubator, and cultivate it for 30 days to obtain a first-class solid strain.

[0062] ) secondary liquid shake flask seed culture

[0063] Put 200ml of liquid medium in a 500mL Erlenmeyer flas...

Embodiment 3

[0073] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:

[0074] 1) Strain selection

[0075] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );

[0076] 2) Strain culture

[0077] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:

[0078] 2.1) Preparation of primary solid bacteria

[0079] The isolated and purified strains were inoculated on the solid medium of a 9 cm petri dish (the medium was: 200.0 g of potato juice, 20.0 g of glucose, 10.0 g of maltose, 10.0 g of peptone, 10.0 g of yeast powder, MgSO 4 1.5 g, KH2PO 4 3.0 g, 20.0 g agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0 M NaOH), put them in an incubator at 18.5 ° C, and cultivate them for 25 days to obtain a first-class solid strain.

[0080] ) secondary liquid shake flask seed culture

[0081] Put 200ml of liquid mediu...

technical field

[0001] The invention belongs to the technical field of biological products and preparation, and in particular relates to an immune enhancer based on Mortierella sinensis and a preparation method. Background technique

[0002] Cordyceps sinensis is a well-known traditional Chinese herbal medicine in my country. It has been used for more than a thousand years. It is as famous as ginseng and antler. It is a complex of fungi formed by Cordyceps sinensis and parasitic insects, including the insect body part full of hyphae (sclerotia) and the fruiting body part grown from the head. Modern pharmacological research shows that Cordyceps sinensis can regulate immunity, delay aging, lower blood pressure, regulate endocrine, eliminate fatigue, prevent and treat tumors, acute or chronic hepatitis, nephritis, antibacterial and antiviral and other pharmacological activities, and has important medical and health care value . Due to the special growth environment of natural...

Examples

Embodiment 1

[0038] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:

[0039] 1) Strain selection

[0040] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );

[0041] 2) Strain culture

[0042] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:

[0043] 2.1) Preparation of primary solid bacteria

[0044]The isolated and purified slant strains were inoculated on the solid medium of a 9 cm petri dish, the medium was: 200.0 g potato juice, 20.0 g glucose, 10.0 g maltose, 10.0 g peptone, 10.0 g yeast powder, MgSO 4 1.5 g, KH2PO 4 Add 3.0 g of agar, 20.0 g of agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0 M NaOH; put it in an incubator at 17°C for 20 days to obtain a first-grade solid strain.

[0045] ) secondary liquid seed culture

[0046] Put 200ml of liquid medium in a 500mL conical flask; each 10...

Embodiment 2

[0055] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:

[0056] 1) Strain selection

[0057] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );

[0058] 2) Strain culture

[0059] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:

[0060] 2.1) Preparation of primary solid bacteria

[0061] The isolated and purified strains were inoculated on the solid medium of a 9 cm petri dish. The medium was: 200.0 g potato juice, 20.0 g glucose, 10.0 g maltose, 10.0 g peptone, 10.0 g yeast powder, MgSO 4 1.5 g, KH2PO 4 3.0 g, 20.0 g agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0 M NaOH; put it in a 20°C incubator, and cultivate it for 30 days to obtain a first-class solid strain.

[0062] ) secondary liquid shake flask seed culture

[0063] Put 200ml of liquid medium in a 500mL Erlenmeyer flas...

Embodiment 3

[0073] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:

[0074] 1) Strain selection

[0075] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );

[0076] 2) Strain culture

[0077] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:

[0078] 2.1) Preparation of primary solid bacteria

[0079] The isolated and purified strains were inoculated on the solid medium of a 9 cm petri dish (the medium was: 200.0 g of potato juice, 20.0 g of glucose, 10.0 g of maltose, 10.0 g of peptone, 10.0 g of yeast powder, MgSO 4 1.5 g, KH2PO 4 3.0 g, 20.0 g agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0 M NaOH), put them in an incubator at 18.5 ° C, and cultivate them for 25 days to obtain a first-class solid strain.

[0080] ) secondary liquid shake flask seed culture

[0081] Put 200ml of liquid mediu...