Fluorescent PCR kit and method for detecting 13 subtypes of human papillomavirus

Abstract

The invention discloses a fluorescent PCR (polymerase chain reaction) kit and a method for detecting 13 subtypes of a human papillomavirus. The kit comprises a PCR liquid, a PCR mixed liquid, a positive standard substance, an internal reference substance and a negative reference substance. According to the fluorescent PCR kit disclosed by the invention, a unique quadruple fluorescent quantitative PCR technology is adopted, PCR amplification primers and detection probes corresponding to the 13 subtypes of the human papillomavirus are respectively designed, six high-risk subtypes of the human papillomavirus are quantitatively detected in two tubes by one step in a typing mode, and unusual seven high-risk subtypes in the Chinese population which are detected in the typing mode are combined.

Description

technical field [0001] The invention relates to a fluorescent PCR kit for detecting 13 subtypes of human papilloma virus and a method thereof, belonging to the field of detection of subtypes of human papilloma virus. The present invention relates to the application of multiple real-time fluorescent quantitative PCR technology in two PCR reaction tubes to rapidly stereotype and quantitatively detect six kinds of high-risk subtypes of human papillomavirus (HPV-16, 18, 31, 33, 52, 58) and Combined typing method for detection of 7 high-risk subtypes of human papillomaviruses (HPV-35, 39, 45, 51, 56, 59, 68) that are not common in Chinese. Background technique [0002] Human Papilloma Virus (HPV) [0003] It is a double-stranded DNA virus belonging to the genus Papillomavirus, and it is a virus that specifically infects the stratified epithelium of the skin or mucous membranes. At present, nearly two hundred subtypes of HPV have been discovered, most of which do not show obviou...

AI Technical Summary

Problems solved by technology

[0009] There are currently two types of HPV diagnostic kits on the market. One is to use PCR fluorescence method and a single fluorescent dye to produce HPV diagnostic kits. Since all probe molecules in the product use the same fluorescent dye, this product cannot distinguish HPV subtypes. type category, and the amount of different subtypes of HPV cannot be detected

Another type of product is produced using gene chips, which can distinguish various types of HPV subtypes, but cannot detect their amounts

In addition, the kit of the gene chip method is complicated to operate, takes a long time to test, is expensive, and is not easy to popularize.

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