The invention relates to a needle filter solid-phase extraction device for a metal organic framework filler, and belongs to the technical field of analytical chemistry sample pretreatment. According to the invention, a metal organic framework dispersion liquid is filled into a needle filter to prepare a solid phase extraction filter head, and a miniaturized solid phase extraction method taking a metal organic framework as an adsorbent and taking the needle filter as an extraction container is developed on the basis of solid phase extraction and adsorbent filling micro-extraction. According tothe method, estrogen can be rapidly enriched through pi-pi action, electrostatic adsorption, space matching and other actions, compared with traditional distributed solid-phase extraction, the extraction time is shortened, the use amount of an extraction agent is reduced, the operation steps are simplified, solid-liquid separation is facilitated, and the device has practical application value.