Automatic device for heterogeneous immunization rapid analysis and use method of automatic device

Abstract

The invention relates to the field of bioanalysis of analytical chemistry, and in particular relates to a device and a method for heterogeneous immunization rapid analysis. The device comprises a capillary tube, a reagent tube and a program-controlled micro pump which are sequentially connected into a channel, and the program-controlled micro pump drives all liquids in the capillary tube and the reagent tube. By using the characteristic of large specific area of the capillary tube, the capillary tube is used as a carrier of a heterogeneous immunoreactions, the incubating time of immunoassay is greatly shortened. The operation in an analysis implementation phase is greatly simplified, the time of on-the-site analysis is reduced, and the full-process automation of liquid control of the liquids in multiple steps of heterogeneous immunoassay is realized. Compared with the traditional heterogeneous immunoassay, the method is remarkably improved in time consumption and operation convenience.

Description

[0001] Technical field [0002] The invention relates to the field of biological analysis of analytical chemistry, in particular to a device and method for rapid analysis of heterogeneous immunity. [0003] Background technique [0004] Immunoassay is an analytical method that uses specific reactions between antigens and antibodies to detect target analytes, and is the most important method for protein detection. Heterogeneous immunoassays, such as Enzyme-linked immunosorbent assay (ELISA) and Fluorescence immunoassay (FIA), because the washing process is added to the analysis process, its specificity and sensitivity are greatly improved. At present, it has become the main technique for quantitative immunoassay in clinical and experimental medicine, and it is widely used in various fields such as heart disease, epidemic disease, and metabolic disease diagnosis. However, current conventional heterogeneous immunoassays based on multiwell plates take a long time for sample turnover a...

AI Technical Summary

Problems solved by technology

Although these rapid and automatic heterogeneous immunoassay methods have effectively solved the problem of long incubation time in the past, there are still major problems in chip processing, process control, and on-site introduction of samples.

In particular, samples and reagents are usually loaded into the chip at the same time, which limits the separation of the preparation process and the use process of the chip, making it difficult to use it in the field of analysis, and the above-mentioned work needs to rely on microfabrication methods, which is costly and isolated. Practical still has a lot of distance

Images