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Thermal Contrast Assays and Readers

A reader, thermal contrast technology, used in instruments, measuring devices, scientific instruments, etc., to solve problems such as end-user use without a proven reliable, cost-effective way

Active Publication Date: 2017-08-25
RGT UNIV OF MINNESOTA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, all of these methods are still under development and have not been proven to be performed in a reliable, cost-effective manner for use by end users in medical sites

Method used

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  • Thermal Contrast Assays and Readers
  • Thermal Contrast Assays and Readers

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0082] Example 1 - Synthesis and Analysis of GNP

[0083] Gold nanoparticles (GNP) synthesis: as described in Frens G. by Perault et al. and Neha et al. (referring to FrensG. .Shah et al. Molecular Pharmaceutics 2012 9(8)2146-2155), synthesized 30nm GNP by the reduction method of chloroauric acid, and then coated polyethylene glycol (PEG) to maintain the stability in aqueous solution. Characterization of GNPs was performed by UV-vis spectrophotometry, atomic emission spectroscopy, dynamic light scattering, and TEM to ensure successful synthesis and to quantify concentration and size. Prepare titrated concentrations of GNP aqueous solutions and transfer 10 µL of each solution as a droplet to a glass slide. Then, the droplet was irradiated with a laser beam from a CW laser (532 nm, Millennia Vs, diode pumped) for 1 min, thereby inducing GNP heating. During laser irradiation, an infrared camera (FLIR ThermoVision TM A20) Remotely measure temperature changes. Determine and pl...

example 3

[0091] Example 3 - Improving Analytical Sensitivity of LFA

[0092] Based on the experimentally measured absorption cross-sectional area of ​​various gold nanoparticles (eg, rods, shells, and spheres), modeling was performed to develop further improvements in the analytical sensitivity of LFA. Gold nanoparticles including gold nanorods, nanoshells and gold nanospheres were evaluated. Such as Figure 7A As shown, typical nanorods and nanoshells generate 4.6 times and 36 times more heat than gold nanospheres, respectively, under the same laser power and nanoparticle concentration. To eliminate particle size effects, the absorption cross-sectional area was normalized by the particle volume (C abs ) for a better estimate of the heat generation capability. The dimensions of the GNPs used for this comparison are: sphere D = 30 nm; nanorod D = 12.7 nm, L = 49.5 nm; nanoshell D core = 120nm (silicon dioxide), D shell = 150 nm (gold). Normalized thermal contrast (ΔT signal ) and...

example 8

[0109] Example 8 - Using Monodisperse Particles

[0110] Nanoparticles were prepared using Frens' method as described in Example 1 and analyzed for dispersibility.

[0111] Such as Figure 12A , Figure 12B and 12C As shown, the synthetic GNPs are more uniformly dispersed than the GNPs in the dipstick.

[0112] Figure 13 is a quantification of the results seen in the TEM image of FIG. 12 . Specifically, increasing the count of GNPs of different sizes, the figure shows a wider distribution in the current dipstick compared to particles synthesized by Frens' method.

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Abstract

An assay for use with a thermal contrast reader is disclosed. In such assays, the test strip includes a material that is thermally responsive if the analyte of interest is present in the sample. The thermal contrast reader includes a housing having an opening for receiving a test strip at a test site; an energy source for the test site; and a thermal sensor for the test site. The thermal sensor is configured to sense heating of the test strip when the heat source is activated at the test site if the target analyte is present in the sample. The thermal sensor may provide a sensor output using a diagnostic circuit coupled to the sensor output and configured to provide a diagnostic output. A diagnostic output may indicate a diagnostic condition of the patient based on the sensor output. The present disclosure also includes methods of detecting target analytes and kits comprising lateral flow assays and thermal contrast readers.

Description

technical field [0001] The present invention relates to assays and readers for detecting analytes in samples. More specifically, the present invention relates to assays and readers that operate based on thermal contrast. Background technique [0002] LFA (Lateral Flow Assay or Lateral Flow Immunoassay, also known as Rapid Diagnostic Test - RDT or Bioassay) technology is used in a wide variety of applications both in and outside of the laboratory setting. In a typical assay, a fluid sample from a patient is applied to a test strip. The sample reacts with the chemicals on the test strip, causing the strip to optically change properties. One can observe visual indications, for example, using a home pregnancy test. However, an assay reader can be used to obtain a more accurate reading. For example, such readers may include optically sensitive sensors that can sense optical changes more accurately and in a more repeatable manner than human observers. One example of a typical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84G01N33/558
CPCG01N21/84G01N33/558G01N21/8483G01N2021/8488G01N25/48G01N25/4806Y02A50/30G01N33/54388
Inventor 约翰·C·比斯乔秦真鹏沃伦·钱塔涅尔·阿克
Owner RGT UNIV OF MINNESOTA