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A method to break the dormancy of Aconitum seeds

A technology of Aconitum aconitum and seeds, applied in the fields of seed and rhizome treatment, application, agriculture, etc., can solve the problems of low germination rate, low reproduction coefficient, poor resistance, etc., and achieve simple treatment methods, broad application prospects, and high-efficiency technical measures Effect

Inactive Publication Date: 2017-04-12
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This characteristic of Aconitum seeds causes the phenomenon of low germination rate, slow germination and irregular germination of Aconitum in the process of seed propagation
Its long dormancy period and low germination rate have become the main bottlenecks in the large-scale production of Aconitum aconitum, which seriously affects the seed propagation effect of Aconitum aconitum.
[0008] At present, although the artificial planting of Aconitum can adopt asexual reproduction methods such as root tuber propagation or bud cutting propagation, there are many defects in the use of asexual reproduction, such as (1) using root tuber propagation, its reproductive coefficient is low, and a wild product can only reproduce 3 to 5 cultivars; (2) After multiple generations of propagation using tubers, the seedlings will degenerate, specifically showing poor resistance, many diseases, and low yield; (3) Propagation using tubers will also affect wild resources. inflict devastating damage

Method used

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  • A method to break the dormancy of Aconitum seeds
  • A method to break the dormancy of Aconitum seeds
  • A method to break the dormancy of Aconitum seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～2

[0042] Take the seeds of Aconitum aconitum, remove impurities, disinfect and sterilize with 1% sodium hypochlorite solution for 5-10 minutes, then rinse with distilled water for 3-5 times, soak the sterilized seeds in gibberellin solutions of different mass concentrations for 24 hours, Then select seeds with the same size and full particles and place them in a petri dish with two layers of filter paper, put 100 seeds in each petri dish, repeat each treatment 3 times, and treat the seeds of each treatment at different temperatures, and use a vernier caliper The embryo length and endosperm length of each treated seed were measured, and the embryo rate, germination rate, and seed rot rate were calculated. The embryo rate is measured at 20 days of culture under this condition; the germination rate is measured at 40 days of culture, and all rotten seeds are measured at 40 days of culture. Example settings are shown in Table 1:

[0043] Table 1 Example Settings

[0044]

Embodiment 3

[0050] The statistical data of Examples 1-2 and Comparative Examples 1-19 were analyzed by one-way analysis of variance using SAS8.0 software, and Fisher's LSD method was used to analyze the significance of differences among different treatment groups. The calculation formula is:

[0051] Embryo rate (%) = embryo length / endosperm length × 100%

[0052] Rotten seed rate (%) = number of moldy and rotten seeds / number of tested seeds × 100%

[0053] Germination rate (%) = number of germinated seeds / number of tested seeds × 100%

[0054] The statistical results are shown in Table 3:

[0055] Table 3 Results Statistics

[0056]

[0057] The results showed that the seeds of Aconitum aconitum had deep dormancy, and under normal conditions, the dormancy period was long and the germination rate was low, while the concentration of 300mg·L -1 ~400mg·L -1 After treatment with gibberellin, the growth rate of embryos was significantly higher than that of the control, and the optima...

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Abstract

The invention relates to the technical field of cultivation of Chinese medicinal materials, in particular to a method for breaking the dormancy of Aconitum aconitum seeds. The method: after the seeds of Aconitum aconitum are sterilized, they are treated with gibberellin at a concentration of 300mg / L-400mg / L; the seeds treated with gibberellin are subjected to high and low temperature alternate cultivation and then cultivated at 0°C to 5°C; high and low temperature During the alternate cultivation process, the temperature of low-temperature cultivation is 14°C-16°C, and the time is 11h-13h; the temperature of high-temperature cultivation is 19°C-21°C, and the time is 11h-13h. The invention combines gibberellin and variable temperature treatment of Aconitum seeds, can quickly break the dormancy of Aconitum seeds, shorten the dormancy period of at least 90 days to about 40 days under traditional technical conditions, and increase the germination rate to 78.65%.

Description

technical field [0001] The invention relates to the technical field of cultivation of Chinese medicinal materials, in particular to a method for breaking the dormancy of Aconitum aconitum seeds. Background technique [0002] The plants of the genus Aconitum in the family Ranunculaceae are divided into Subgenus Aconitum, Subgen Paraconitum and Subgen Gymnaconitum. Among them, the subgenus Aconitum is divided into Aconitum group and Aconitum group, and Aconitum group is divided into Xing'an Aconitum group, Short handle Aconitum group, Brown Purple Aconitum group, Baoshan Aconitum group, Junggar Aconitum group Department of Aconitum, Rock Aconitum, Round Leaf Aconitum, Xianzhu Aconitum, Ganqing Aconitum, Mann Aconitum. Plants of the genus Aconitum contain alkaloids such as aconitine, and most types of roots are highly toxic. About 36 species of Aconitum are available for medicinal use. However, different species of aconitum have different morphological characteristics, growt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00
Inventor 邵财王英平张亚玉刘继永张浩孙海关一鸣吴连举
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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